Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1220

Experiment: Ying_Nucleobase14 rep B; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1219 and Pf6N2E2_1220 are separated by 63 nucleotidesPf6N2E2_1220 and Pf6N2E2_1221 are separated by 319 nucleotides Pf6N2E2_1219 - FIG00955186: hypothetical protein, at 1,425,925 to 1,427,115 _1219 Pf6N2E2_1220 - hypothetical protein, at 1,427,179 to 1,427,340 _1220 Pf6N2E2_1221 - sodium/hydrogen exchanger family protein, at 1,427,660 to 1,428,997 _1221 Position (kb) 1427 1428Strain fitness (log2 ratio) -2 -1 0 1 2at 1426.377 kb on - strand, within Pf6N2E2_1219at 1426.568 kb on - strand, within Pf6N2E2_1219at 1426.568 kb on - strand, within Pf6N2E2_1219at 1426.575 kb on + strand, within Pf6N2E2_1219at 1426.625 kb on + strand, within Pf6N2E2_1219at 1426.626 kb on - strand, within Pf6N2E2_1219at 1426.727 kb on + strand, within Pf6N2E2_1219at 1426.727 kb on + strand, within Pf6N2E2_1219at 1426.775 kb on + strand, within Pf6N2E2_1219at 1426.775 kb on + strand, within Pf6N2E2_1219at 1426.886 kb on + strand, within Pf6N2E2_1219at 1426.886 kb on + strand, within Pf6N2E2_1219at 1426.887 kb on - strand, within Pf6N2E2_1219at 1426.887 kb on - strand, within Pf6N2E2_1219at 1426.940 kb on + strand, within Pf6N2E2_1219at 1426.940 kb on + strand, within Pf6N2E2_1219at 1426.940 kb on + strand, within Pf6N2E2_1219at 1426.941 kb on - strand, within Pf6N2E2_1219at 1426.991 kb on + strand, within Pf6N2E2_1219at 1426.991 kb on + strand, within Pf6N2E2_1219at 1426.991 kb on + strand, within Pf6N2E2_1219at 1426.992 kb on - strand, within Pf6N2E2_1219at 1426.992 kb on - strand, within Pf6N2E2_1219at 1427.063 kb on + strandat 1427.064 kb on - strandat 1427.064 kb on - strandat 1427.064 kb on - strandat 1427.231 kb on + strand, within Pf6N2E2_1220at 1427.231 kb on + strand, within Pf6N2E2_1220at 1427.232 kb on - strand, within Pf6N2E2_1220at 1427.232 kb on - strand, within Pf6N2E2_1220at 1427.260 kb on - strand, within Pf6N2E2_1220at 1427.310 kb on + strand, within Pf6N2E2_1220at 1427.727 kb on - strandat 1427.727 kb on - strandat 1427.727 kb on - strandat 1427.936 kb on + strand, within Pf6N2E2_1221at 1427.996 kb on + strand, within Pf6N2E2_1221at 1427.996 kb on + strand, within Pf6N2E2_1221at 1428.052 kb on + strand, within Pf6N2E2_1221at 1428.053 kb on - strand, within Pf6N2E2_1221

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 1
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1,426,377 - Pf6N2E2_1219 0.38 +0.5
1,426,568 - Pf6N2E2_1219 0.54 +0.8
1,426,568 - Pf6N2E2_1219 0.54 +0.8
1,426,575 + Pf6N2E2_1219 0.55 -1.6
1,426,625 + Pf6N2E2_1219 0.59 +0.8
1,426,626 - Pf6N2E2_1219 0.59 +0.8
1,426,727 + Pf6N2E2_1219 0.67 +0.8
1,426,727 + Pf6N2E2_1219 0.67 +0.8
1,426,775 + Pf6N2E2_1219 0.71 +0.8
1,426,775 + Pf6N2E2_1219 0.71 -0.2
1,426,886 + Pf6N2E2_1219 0.81 +0.8
1,426,886 + Pf6N2E2_1219 0.81 -2.0
1,426,887 - Pf6N2E2_1219 0.81 +0.8
1,426,887 - Pf6N2E2_1219 0.81 +0.8
1,426,940 + Pf6N2E2_1219 0.85 -0.6
1,426,940 + Pf6N2E2_1219 0.85 +1.8
1,426,940 + Pf6N2E2_1219 0.85 -0.4
1,426,941 - Pf6N2E2_1219 0.85 -1.2
1,426,991 + Pf6N2E2_1219 0.90 +0.8
1,426,991 + Pf6N2E2_1219 0.90 +0.8
1,426,991 + Pf6N2E2_1219 0.90 +0.8
1,426,992 - Pf6N2E2_1219 0.90 -0.2
1,426,992 - Pf6N2E2_1219 0.90 -0.2
1,427,063 + +0.8
1,427,064 - +0.8
1,427,064 - -0.2
1,427,064 - +0.2
1,427,231 + Pf6N2E2_1220 0.32 +0.8
1,427,231 + Pf6N2E2_1220 0.32 +0.8
1,427,232 - Pf6N2E2_1220 0.33 -0.2
1,427,232 - Pf6N2E2_1220 0.33 -2.5
1,427,260 - Pf6N2E2_1220 0.50 -0.2
1,427,310 + Pf6N2E2_1220 0.81 -1.6
1,427,727 - +0.8
1,427,727 - -0.2
1,427,727 - +0.8
1,427,936 + Pf6N2E2_1221 0.21 +0.8
1,427,996 + Pf6N2E2_1221 0.25 +0.8
1,427,996 + Pf6N2E2_1221 0.25 +0.5
1,428,052 + Pf6N2E2_1221 0.29 -0.2
1,428,053 - Pf6N2E2_1221 0.29 -0.2

Or see this region's nucleotide sequence