Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5131

Experiment: Ying_sugar7 rep B; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5129 and Pf6N2E2_6146 are separated by 415 nucleotidesPf6N2E2_6146 and Pf6N2E2_6147 are separated by 10 nucleotidesPf6N2E2_6147 and Pf6N2E2_6148 are separated by 8 nucleotidesPf6N2E2_6148 and Pf6N2E2_6149 are separated by 96 nucleotidesPf6N2E2_6149 and Pf6N2E2_5130 overlap by 9 nucleotidesPf6N2E2_5130 and Pf6N2E2_5131 are separated by 30 nucleotidesPf6N2E2_5131 and Pf6N2E2_5132 are separated by 139 nucleotides Pf6N2E2_5129 - ClpB protein, at 5,913,135 to 5,915,699 _5129 Pf6N2E2_6146 - tRNA-Asn-GTT, at 5,916,115 to 5,916,187 _6146 Pf6N2E2_6147 - tRNA-Pro-TGG, at 5,916,198 to 5,916,271 _6147 Pf6N2E2_6148 - tRNA-Lys-TTT, at 5,916,280 to 5,916,352 _6148 Pf6N2E2_6149 - tRNA-Pro-TGG, at 5,916,449 to 5,916,522 _6149 Pf6N2E2_5130 - hypothetical protein, at 5,916,514 to 5,916,642 _5130 Pf6N2E2_5131 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 5,916,673 to 5,918,298 _5131 Pf6N2E2_5132 - NADH dehydrogenase (EC 1.6.99.3), at 5,918,438 to 5,919,736 _5132 Position (kb) 5916 5917 5918 5919Strain fitness (log2 ratio) -1 0 1at 5915.936 kb on + strandat 5915.936 kb on + strandat 5916.387 kb on + strandat 5916.526 kb on + strandat 5916.526 kb on + strandat 5916.526 kb on + strandat 5916.541 kb on + strand, within Pf6N2E2_5130at 5916.541 kb on + strand, within Pf6N2E2_5130at 5916.551 kb on + strand, within Pf6N2E2_5130at 5916.657 kb on - strandat 5916.672 kb on + strandat 5916.913 kb on - strand, within Pf6N2E2_5131at 5916.913 kb on - strand, within Pf6N2E2_5131at 5916.922 kb on + strand, within Pf6N2E2_5131at 5917.015 kb on + strand, within Pf6N2E2_5131at 5917.015 kb on + strand, within Pf6N2E2_5131at 5917.046 kb on - strand, within Pf6N2E2_5131at 5917.225 kb on + strand, within Pf6N2E2_5131at 5917.226 kb on - strand, within Pf6N2E2_5131at 5917.311 kb on + strand, within Pf6N2E2_5131at 5917.338 kb on + strand, within Pf6N2E2_5131at 5917.339 kb on - strand, within Pf6N2E2_5131at 5917.339 kb on - strand, within Pf6N2E2_5131at 5918.034 kb on + strand, within Pf6N2E2_5131at 5918.300 kb on + strandat 5918.300 kb on + strandat 5918.432 kb on - strandat 5918.432 kb on - strandat 5918.518 kb on - strandat 5918.518 kb on - strandat 5918.518 kb on - strandat 5918.542 kb on - strandat 5918.542 kb on - strandat 5918.553 kb on + strandat 5918.553 kb on + strandat 5918.553 kb on + strandat 5918.553 kb on + strandat 5918.554 kb on - strandat 5918.554 kb on - strandat 5918.655 kb on + strand, within Pf6N2E2_5132at 5918.656 kb on - strand, within Pf6N2E2_5132at 5918.664 kb on + strand, within Pf6N2E2_5132at 5918.664 kb on + strand, within Pf6N2E2_5132at 5918.664 kb on + strand, within Pf6N2E2_5132at 5918.664 kb on + strand, within Pf6N2E2_5132at 5918.665 kb on - strand, within Pf6N2E2_5132at 5918.665 kb on - strand, within Pf6N2E2_5132at 5918.665 kb on - strand, within Pf6N2E2_5132at 5918.665 kb on - strand, within Pf6N2E2_5132at 5918.665 kb on - strand, within Pf6N2E2_5132at 5918.665 kb on - strand, within Pf6N2E2_5132at 5918.842 kb on + strand, within Pf6N2E2_5132at 5918.843 kb on - strand, within Pf6N2E2_5132at 5919.062 kb on - strand, within Pf6N2E2_5132at 5919.062 kb on - strand, within Pf6N2E2_5132at 5919.173 kb on - strand, within Pf6N2E2_5132at 5919.173 kb on - strand, within Pf6N2E2_5132at 5919.173 kb on - strand, within Pf6N2E2_5132at 5919.273 kb on + strand, within Pf6N2E2_5132

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 1
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5,915,936 + +0.0
5,915,936 + +1.0
5,916,387 + -1.0
5,916,526 + +1.0
5,916,526 + +1.0
5,916,526 + -1.2
5,916,541 + Pf6N2E2_5130 0.21 -0.9
5,916,541 + Pf6N2E2_5130 0.21 -0.4
5,916,551 + Pf6N2E2_5130 0.29 -1.1
5,916,657 - +0.3
5,916,672 + +1.0
5,916,913 - Pf6N2E2_5131 0.15 +1.0
5,916,913 - Pf6N2E2_5131 0.15 -0.6
5,916,922 + Pf6N2E2_5131 0.15 -0.8
5,917,015 + Pf6N2E2_5131 0.21 -1.0
5,917,015 + Pf6N2E2_5131 0.21 -1.3
5,917,046 - Pf6N2E2_5131 0.23 -1.5
5,917,225 + Pf6N2E2_5131 0.34 -1.4
5,917,226 - Pf6N2E2_5131 0.34 -0.6
5,917,311 + Pf6N2E2_5131 0.39 +0.0
5,917,338 + Pf6N2E2_5131 0.41 +1.0
5,917,339 - Pf6N2E2_5131 0.41 -1.3
5,917,339 - Pf6N2E2_5131 0.41 -1.5
5,918,034 + Pf6N2E2_5131 0.84 +1.0
5,918,300 + +1.0
5,918,300 + +1.0
5,918,432 - +0.0
5,918,432 - +0.2
5,918,518 - +0.0
5,918,518 - -1.2
5,918,518 - -1.3
5,918,542 - -0.6
5,918,542 - -0.8
5,918,553 + +1.0
5,918,553 + +0.0
5,918,553 + -1.1
5,918,553 + -0.7
5,918,554 - -0.6
5,918,554 - -0.6
5,918,655 + Pf6N2E2_5132 0.17 -0.7
5,918,656 - Pf6N2E2_5132 0.17 +0.0
5,918,664 + Pf6N2E2_5132 0.17 +1.0
5,918,664 + Pf6N2E2_5132 0.17 -0.7
5,918,664 + Pf6N2E2_5132 0.17 +1.0
5,918,664 + Pf6N2E2_5132 0.17 -0.9
5,918,665 - Pf6N2E2_5132 0.17 +1.0
5,918,665 - Pf6N2E2_5132 0.17 +0.0
5,918,665 - Pf6N2E2_5132 0.17 +1.0
5,918,665 - Pf6N2E2_5132 0.17 +1.0
5,918,665 - Pf6N2E2_5132 0.17 +0.0
5,918,665 - Pf6N2E2_5132 0.17 +1.0
5,918,842 + Pf6N2E2_5132 0.31 +1.4
5,918,843 - Pf6N2E2_5132 0.31 +0.0
5,919,062 - Pf6N2E2_5132 0.48 -0.1
5,919,062 - Pf6N2E2_5132 0.48 +0.0
5,919,173 - Pf6N2E2_5132 0.57 +1.0
5,919,173 - Pf6N2E2_5132 0.57 -0.3
5,919,173 - Pf6N2E2_5132 0.57 -0.3
5,919,273 + Pf6N2E2_5132 0.64 +0.4

Or see this region's nucleotide sequence