Experiment: PHYTO3 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28495 and BPHYT_RS28500 are separated by 225 nucleotides BPHYT_RS28500 and BPHYT_RS28505 are separated by 16 nucleotides BPHYT_RS28505 and BPHYT_RS28510 are separated by 69 nucleotides
BPHYT_RS28495: BPHYT_RS28495 - peptidase M24, at 1,947,555 to 1,948,721
_RS28495
BPHYT_RS28500: BPHYT_RS28500 - LysR family transcriptional regulator, at 1,948,947 to 1,949,903
_RS28500
BPHYT_RS28505: BPHYT_RS28505 - 5-deoxyglucuronate isomerase, at 1,949,920 to 1,950,750
_RS28505
BPHYT_RS28510: BPHYT_RS28510 - dioxygenase, at 1,950,820 to 1,952,298
_RS28510
Position (kb) 1948
1949
1950 Strain fitness (log2 ratio) -2
-1
0
1 at 1947.971 kb on - strand, within BPHYT_RS28495 at 1947.971 kb on - strand, within BPHYT_RS28495 at 1948.050 kb on - strand, within BPHYT_RS28495 at 1948.343 kb on + strand, within BPHYT_RS28495 at 1948.343 kb on + strand, within BPHYT_RS28495 at 1948.346 kb on - strand, within BPHYT_RS28495 at 1948.428 kb on - strand, within BPHYT_RS28495 at 1948.570 kb on + strand, within BPHYT_RS28495 at 1948.680 kb on - strand at 1948.828 kb on + strand at 1948.829 kb on - strand at 1948.829 kb on - strand at 1948.829 kb on - strand at 1948.831 kb on + strand at 1948.831 kb on + strand at 1948.831 kb on + strand at 1948.832 kb on - strand at 1949.451 kb on + strand, within BPHYT_RS28500 at 1949.452 kb on - strand, within BPHYT_RS28500 at 1950.033 kb on - strand, within BPHYT_RS28505 at 1950.041 kb on + strand, within BPHYT_RS28505 at 1950.186 kb on - strand, within BPHYT_RS28505
Per-strain Table
Position Strand Gene LocusTag Fraction PHYTO3 MOI 0.1 remove 1,947,971 - BPHYT_RS28495 0.36 -0.8 1,947,971 - BPHYT_RS28495 0.36 -0.8 1,948,050 - BPHYT_RS28495 0.42 -0.9 1,948,343 + BPHYT_RS28495 0.68 +0.3 1,948,343 + BPHYT_RS28495 0.68 +1.3 1,948,346 - BPHYT_RS28495 0.68 +0.9 1,948,428 - BPHYT_RS28495 0.75 +0.3 1,948,570 + BPHYT_RS28495 0.87 +0.3 1,948,680 - -0.1 1,948,828 + +0.1 1,948,829 - +0.8 1,948,829 - -0.7 1,948,829 - -0.2 1,948,831 + -1.1 1,948,831 + +0.5 1,948,831 + -0.5 1,948,832 - +0.5 1,949,451 + BPHYT_RS28500 0.53 -1.1 1,949,452 - BPHYT_RS28500 0.53 +0.9 1,950,033 - BPHYT_RS28505 0.14 -0.4 1,950,041 + BPHYT_RS28505 0.15 -0.5 1,950,186 - BPHYT_RS28505 0.32 -1.8
Or see this region's nucleotide sequence