Experiment: L-tyrosine (C) 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS02270 and PFLU_RS02275 are separated by 11 nucleotides PFLU_RS02275 and PFLU_RS02280 are separated by 383 nucleotides
PFLU_RS02270: PFLU_RS02270 - dihydrolipoyllysine-residue acetyltransferase, at 515,559 to 517,208
_RS02270
PFLU_RS02275: PFLU_RS02275 - pyruvate dehydrogenase (acetyl-transferring), homodimeric type, at 517,220 to 519,865
_RS02275
PFLU_RS02280: PFLU_RS02280 - bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase, at 520,249 to 523,188
_RS02280
Position (kb) 517
518
519
520 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 516.359 kb on - strand, within PFLU_RS02270 at 516.624 kb on + strand, within PFLU_RS02270 at 517.159 kb on - strand at 517.288 kb on + strand at 517.289 kb on - strand at 517.289 kb on - strand at 517.749 kb on + strand, within PFLU_RS02275 at 517.750 kb on - strand, within PFLU_RS02275 at 517.827 kb on + strand, within PFLU_RS02275 at 518.220 kb on + strand, within PFLU_RS02275 at 518.220 kb on + strand, within PFLU_RS02275 at 518.221 kb on - strand, within PFLU_RS02275 at 518.221 kb on - strand, within PFLU_RS02275 at 518.275 kb on - strand, within PFLU_RS02275 at 518.467 kb on + strand, within PFLU_RS02275 at 518.468 kb on - strand, within PFLU_RS02275 at 518.784 kb on + strand, within PFLU_RS02275 at 518.785 kb on - strand, within PFLU_RS02275 at 518.907 kb on + strand, within PFLU_RS02275 at 519.273 kb on + strand, within PFLU_RS02275 at 519.469 kb on - strand, within PFLU_RS02275 at 519.514 kb on - strand, within PFLU_RS02275 at 519.531 kb on + strand, within PFLU_RS02275 at 519.532 kb on - strand, within PFLU_RS02275 at 519.627 kb on - strand at 519.778 kb on - strand at 519.940 kb on + strand at 519.941 kb on - strand at 519.941 kb on - strand at 519.941 kb on - strand at 519.979 kb on - strand at 519.984 kb on + strand at 520.020 kb on + strand at 520.020 kb on + strand at 520.020 kb on + strand at 520.021 kb on - strand at 520.021 kb on - strand at 520.021 kb on - strand at 520.021 kb on - strand at 520.021 kb on - strand at 520.021 kb on - strand at 520.021 kb on - strand at 520.021 kb on - strand at 520.021 kb on - strand at 520.021 kb on - strand at 520.021 kb on - strand at 520.022 kb on + strand at 520.022 kb on + strand at 520.022 kb on + strand at 520.022 kb on + strand at 520.022 kb on + strand at 520.022 kb on + strand at 520.022 kb on + strand at 520.023 kb on - strand at 520.023 kb on - strand at 520.023 kb on - strand at 520.023 kb on - strand at 520.023 kb on - strand at 520.024 kb on + strand at 520.024 kb on + strand at 520.024 kb on + strand at 520.024 kb on + strand at 520.024 kb on + strand at 520.024 kb on + strand at 520.024 kb on + strand at 520.024 kb on + strand at 520.024 kb on + strand at 520.024 kb on + strand at 520.024 kb on + strand at 520.024 kb on + strand at 520.024 kb on + strand at 520.024 kb on + strand at 520.024 kb on + strand at 520.025 kb on - strand at 520.025 kb on - strand at 520.025 kb on - strand at 520.025 kb on - strand at 520.025 kb on - strand at 520.025 kb on - strand at 520.025 kb on - strand at 520.025 kb on - strand at 520.025 kb on - strand at 520.025 kb on - strand at 520.025 kb on - strand at 520.025 kb on - strand at 520.025 kb on - strand at 520.025 kb on - strand at 520.025 kb on - strand at 520.025 kb on - strand at 520.030 kb on + strand at 520.040 kb on - strand at 520.040 kb on - strand at 520.055 kb on + strand at 520.081 kb on - strand at 520.081 kb on - strand at 520.081 kb on - strand at 520.164 kb on - strand at 520.191 kb on + strand at 520.191 kb on + strand at 520.191 kb on + strand at 520.192 kb on - strand at 520.226 kb on + strand at 520.226 kb on + strand at 520.226 kb on + strand at 520.226 kb on + strand at 520.226 kb on + strand at 520.227 kb on - strand at 520.227 kb on - strand at 520.243 kb on + strand at 520.243 kb on + strand at 520.244 kb on - strand at 520.244 kb on - strand at 520.244 kb on - strand at 520.244 kb on - strand at 520.244 kb on - strand at 520.693 kb on + strand, within PFLU_RS02280 at 520.694 kb on - strand, within PFLU_RS02280 at 520.694 kb on - strand, within PFLU_RS02280 at 520.705 kb on + strand, within PFLU_RS02280 at 520.705 kb on + strand, within PFLU_RS02280 at 520.705 kb on + strand, within PFLU_RS02280 at 520.705 kb on + strand, within PFLU_RS02280 at 520.706 kb on - strand, within PFLU_RS02280 at 520.706 kb on - strand, within PFLU_RS02280 at 520.706 kb on - strand, within PFLU_RS02280 at 520.706 kb on - strand, within PFLU_RS02280 at 520.706 kb on - strand, within PFLU_RS02280 at 520.721 kb on + strand, within PFLU_RS02280 at 520.721 kb on + strand, within PFLU_RS02280 at 520.721 kb on + strand, within PFLU_RS02280 at 520.722 kb on - strand, within PFLU_RS02280 at 520.722 kb on - strand, within PFLU_RS02280 at 520.722 kb on - strand, within PFLU_RS02280 at 520.722 kb on - strand, within PFLU_RS02280 at 520.722 kb on - strand, within PFLU_RS02280 at 520.722 kb on - strand, within PFLU_RS02280 at 520.737 kb on + strand, within PFLU_RS02280 at 520.843 kb on + strand, within PFLU_RS02280
Per-strain Table
Position Strand Gene LocusTag Fraction L-tyrosine (C) 10 mM remove 516,359 - PFLU_RS02270 0.48 -0.8 516,624 + PFLU_RS02270 0.65 -2.1 517,159 - -0.8 517,288 + -1.3 517,289 - +1.5 517,289 - +0.5 517,749 + PFLU_RS02275 0.20 +0.5 517,750 - PFLU_RS02275 0.20 +0.2 517,827 + PFLU_RS02275 0.23 -0.1 518,220 + PFLU_RS02275 0.38 -0.1 518,220 + PFLU_RS02275 0.38 +0.5 518,221 - PFLU_RS02275 0.38 +1.5 518,221 - PFLU_RS02275 0.38 -1.1 518,275 - PFLU_RS02275 0.40 +1.5 518,467 + PFLU_RS02275 0.47 +0.5 518,468 - PFLU_RS02275 0.47 -1.1 518,784 + PFLU_RS02275 0.59 -0.8 518,785 - PFLU_RS02275 0.59 -0.5 518,907 + PFLU_RS02275 0.64 -0.1 519,273 + PFLU_RS02275 0.78 +3.1 519,469 - PFLU_RS02275 0.85 -0.1 519,514 - PFLU_RS02275 0.87 -0.1 519,531 + PFLU_RS02275 0.87 +2.5 519,532 - PFLU_RS02275 0.87 +2.5 519,627 - +1.5 519,778 - +0.5 519,940 + -0.5 519,941 - +0.9 519,941 - -0.7 519,941 - -1.2 519,979 - +1.0 519,984 + +1.5 520,020 + +0.6 520,020 + +0.6 520,020 + +0.3 520,021 - -0.3 520,021 - -0.6 520,021 - -0.4 520,021 - +0.8 520,021 - -0.5 520,021 - -0.6 520,021 - +1.5 520,021 - -1.1 520,021 - +0.3 520,021 - -0.8 520,021 - -0.9 520,022 + -0.2 520,022 + +0.9 520,022 + +0.5 520,022 + +1.5 520,022 + -0.4 520,022 + -1.7 520,022 + -0.1 520,023 - -0.4 520,023 - -1.9 520,023 - -1.1 520,023 - +0.0 520,023 - +1.2 520,024 + -1.5 520,024 + -2.2 520,024 + -1.2 520,024 + -0.5 520,024 + -0.5 520,024 + +0.7 520,024 + -0.1 520,024 + -0.1 520,024 + +0.2 520,024 + -0.8 520,024 + -1.8 520,024 + -0.4 520,024 + -1.7 520,024 + -1.1 520,024 + -0.6 520,025 - -0.2 520,025 - +0.1 520,025 - +0.7 520,025 - -0.9 520,025 - -0.9 520,025 - -0.0 520,025 - -0.9 520,025 - +1.0 520,025 - -0.4 520,025 - -1.3 520,025 - -0.3 520,025 - +0.4 520,025 - -0.7 520,025 - -2.4 520,025 - +0.9 520,025 - -0.4 520,030 + +0.9 520,040 - -0.6 520,040 - +0.5 520,055 + +0.8 520,081 - -0.8 520,081 - +0.5 520,081 - -0.5 520,164 - +0.2 520,191 + +0.8 520,191 + -0.2 520,191 + +1.8 520,192 - -0.6 520,226 + -3.0 520,226 + -0.1 520,226 + -1.0 520,226 + -1.8 520,226 + +0.0 520,227 - -1.1 520,227 - -0.1 520,243 + +0.1 520,243 + -1.5 520,244 - -1.1 520,244 - +0.3 520,244 - +0.4 520,244 - +0.8 520,244 - -0.1 520,693 + PFLU_RS02280 0.15 -0.1 520,694 - PFLU_RS02280 0.15 -0.5 520,694 - PFLU_RS02280 0.15 -2.3 520,705 + PFLU_RS02280 0.16 +0.1 520,705 + PFLU_RS02280 0.16 -0.6 520,705 + PFLU_RS02280 0.16 -2.2 520,705 + PFLU_RS02280 0.16 -1.7 520,706 - PFLU_RS02280 0.16 -1.7 520,706 - PFLU_RS02280 0.16 -3.2 520,706 - PFLU_RS02280 0.16 -1.9 520,706 - PFLU_RS02280 0.16 -1.6 520,706 - PFLU_RS02280 0.16 -0.5 520,721 + PFLU_RS02280 0.16 +1.2 520,721 + PFLU_RS02280 0.16 -1.3 520,721 + PFLU_RS02280 0.16 +0.6 520,722 - PFLU_RS02280 0.16 +0.5 520,722 - PFLU_RS02280 0.16 -0.6 520,722 - PFLU_RS02280 0.16 -1.9 520,722 - PFLU_RS02280 0.16 +0.1 520,722 - PFLU_RS02280 0.16 +0.9 520,722 - PFLU_RS02280 0.16 +2.1 520,737 + PFLU_RS02280 0.17 -1.6 520,843 + PFLU_RS02280 0.20 -1.7
Or see this region's nucleotide sequence