Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS28070

Experiment: L-Isoleucine (C) 10 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	L-Isoleucine (C) 10 mM
6,250,661	+		PFLU_RS28060	0.71	+1.4
6,250,661	+		PFLU_RS28060	0.71	+0.5
6,250,661	+		PFLU_RS28060	0.71	+0.4
6,250,661	+		PFLU_RS28060	0.71	-1.2
6,250,661	+		PFLU_RS28060	0.71	-0.2
6,250,661	+		PFLU_RS28060	0.71	-0.4
6,250,662	-		PFLU_RS28060	0.71	-1.0
6,250,662	-		PFLU_RS28060	0.71	+0.3
6,250,662	-		PFLU_RS28060	0.71	+0.3
6,250,662	-		PFLU_RS28060	0.71	+0.1
6,250,662	-		PFLU_RS28060	0.71	-0.6
6,250,662	-		PFLU_RS28060	0.71	-0.5
6,250,662	-		PFLU_RS28060	0.71	+2.6
6,250,662	-		PFLU_RS28060	0.71	+3.0
6,250,678	+		PFLU_RS28060	0.73	-0.4
6,250,678	+		PFLU_RS28060	0.73	-1.0
6,250,679	-		PFLU_RS28060	0.73	-0.6
6,250,679	-		PFLU_RS28060	0.73	+0.5
6,250,700	+		PFLU_RS28060	0.77	+0.8
6,250,701	-		PFLU_RS28060	0.77	-0.8
6,250,701	-		PFLU_RS28060	0.77	-0.0
6,250,701	-		PFLU_RS28060	0.77	-1.4
6,250,760	+		PFLU_RS28060	0.86	-0.3
6,250,760	+		PFLU_RS28060	0.86	-0.2
6,250,760	+		PFLU_RS28060	0.86	-0.4
6,250,760	+		PFLU_RS28060	0.86	-0.5
6,250,761	-		PFLU_RS28060	0.86	-0.1
6,250,761	-		PFLU_RS28060	0.86	+0.1
6,250,761	-		PFLU_RS28060	0.86	+0.4
6,250,761	-		PFLU_RS28060	0.86	-1.3
6,250,778	+		PFLU_RS28060	0.88	-1.1
6,250,778	+		PFLU_RS28060	0.88	-0.4
6,250,778	+		PFLU_RS28060	0.88	+0.7
6,250,778	+		PFLU_RS28060	0.88	+0.0
6,250,779	-		PFLU_RS28060	0.88	+0.6
6,250,779	-		PFLU_RS28060	0.88	+0.2
6,250,779	-		PFLU_RS28060	0.88	+0.8
6,250,779	-		PFLU_RS28060	0.88	-1.3
6,250,779	-		PFLU_RS28060	0.88	+0.2
6,250,779	-		PFLU_RS28060	0.88	+0.1
6,250,779	-		PFLU_RS28060	0.88	-1.5
6,250,779	-		PFLU_RS28060	0.88	+1.7
6,250,779	-		PFLU_RS28060	0.88	+0.7
6,250,842	+				-0.4
6,250,842	+				-1.2
6,250,842	+				+0.4
6,250,842	+				-1.2
6,250,842	+				-0.4
6,250,842	+				-0.1
6,250,842	+				-1.4
6,250,842	+				-0.7
6,250,842	+				-0.2
6,250,842	+				-1.3
6,250,842	+				+0.4
6,250,842	+				-2.1
6,250,842	+				-0.4
6,250,842	+				+0.8
6,250,843	-				+0.3
6,250,843	-				-0.2
6,250,843	-				-1.3
6,250,843	-				-0.1
6,250,843	-				-0.0
6,250,843	-				-1.7
6,250,843	-				+0.2
6,250,843	-				+1.4
6,250,843	-				-0.8
6,250,869	-				-0.4
6,250,907	+				+0.2
6,250,907	+				+0.7
6,250,907	+				+0.2
6,250,907	+				+0.2
6,250,907	+				-0.6
6,250,907	+				+0.6
6,250,908	-				+0.1
6,250,908	-				-0.3
6,250,908	-				+0.9
6,250,908	-				-0.6
6,250,908	-				+0.0
6,250,991	+		PFLU_RS28065	0.19	-1.0
6,250,997	-		PFLU_RS28065	0.20	+0.4
6,251,005	+		PFLU_RS28065	0.22	-0.6
6,251,083	+		PFLU_RS28065	0.42	+0.5
6,251,084	-		PFLU_RS28065	0.42	-0.0
6,251,133	+		PFLU_RS28065	0.54	-1.0
6,251,134	-		PFLU_RS28065	0.54	-0.3
6,251,134	-		PFLU_RS28065	0.54	-0.0
6,251,134	-		PFLU_RS28065	0.54	+0.0
6,251,134	-		PFLU_RS28065	0.54	+0.9
6,251,134	-		PFLU_RS28065	0.54	-0.0
6,251,145	+		PFLU_RS28065	0.57	+1.4
6,251,145	+		PFLU_RS28065	0.57	-0.1
6,251,146	-		PFLU_RS28065	0.57	+0.6
6,251,185	+		PFLU_RS28065	0.67	-0.0
6,251,185	+		PFLU_RS28065	0.67	-1.0
6,251,185	+		PFLU_RS28065	0.67	-0.2
6,251,186	-		PFLU_RS28065	0.67	-0.2
6,251,221	+		PFLU_RS28065	0.76	+0.1
6,251,221	+		PFLU_RS28065	0.76	-1.8
6,251,221	+		PFLU_RS28065	0.76	+1.2
6,251,222	-		PFLU_RS28065	0.76	+0.4
6,251,222	-		PFLU_RS28065	0.76	+1.2
6,251,222	-		PFLU_RS28065	0.76	+0.8
6,251,230	+		PFLU_RS28065	0.78	+3.0
6,251,231	-		PFLU_RS28065	0.78	+0.1
6,251,234	+		PFLU_RS28065	0.79	-0.4
6,251,234	+		PFLU_RS28065	0.79	+0.0
6,251,234	+		PFLU_RS28065	0.79	-0.7
6,251,235	-		PFLU_RS28065	0.79	+0.1
6,251,235	-		PFLU_RS28065	0.79	-0.2
6,251,236	+		PFLU_RS28065	0.80	-0.9
6,251,236	+		PFLU_RS28065	0.80	+0.1
6,251,236	+		PFLU_RS28065	0.80	+0.1
6,251,237	-		PFLU_RS28065	0.80	-0.8
6,251,237	-		PFLU_RS28065	0.80	-0.5
6,251,237	-		PFLU_RS28065	0.80	-0.2
6,251,276	+		PFLU_RS28065	0.90	+0.0
6,251,276	+		PFLU_RS28065	0.90	-0.2
6,251,276	+		PFLU_RS28065	0.90	-0.0
6,251,277	-				+1.0
6,251,277	-				+2.7
6,251,308	+				+0.2
6,251,338	-				-1.6
6,251,368	+				+0.1
6,251,368	+				+0.0
6,251,368	+				-0.0
6,251,377	+				-0.6
6,251,377	+				-0.4
6,251,377	+				-2.2
6,251,377	+				+0.8
6,251,378	-				+0.1
6,251,378	-				-0.4
6,251,502	+				+2.4
6,251,503	-				+0.4
6,251,504	+				+1.1
6,251,525	+				-1.3
6,251,531	+				-1.0
6,251,531	+				-1.6
6,251,531	+				-1.2
6,251,531	+				+0.5
6,251,531	+				+3.0
6,251,532	-				-0.1
6,251,532	-				-0.8
6,251,532	-				-0.4
6,251,532	-				+2.0
6,251,602	+				+0.4
6,251,633	+				-2.3
6,251,642	+				-1.1
6,251,643	-				+2.9
6,251,643	-				+0.7
6,251,643	-				-1.0
6,251,643	-				+2.5
6,252,773	-				+0.5
6,252,773	-				-0.2
6,252,782	-				+0.7
6,252,782	-				+0.0
6,252,782	-				+0.4
6,252,782	-				+0.3
6,252,782	-				+0.3
6,252,971	-				-0.5
6,252,971	-				-0.3
6,252,971	-				+2.0
6,252,993	+				-0.4
6,253,003	+				-0.3
6,253,003	+				+0.0
6,253,003	+				-0.4
6,253,003	+				-0.2
6,253,003	+				-1.2
6,253,003	+				+0.7
6,253,041	+		PFLU_RS28075	0.15	+1.7
6,253,041	+		PFLU_RS28075	0.15	-1.2
6,253,092	+		PFLU_RS28075	0.30	+0.1
6,253,092	+		PFLU_RS28075	0.30	+0.3
6,253,092	+		PFLU_RS28075	0.30	+0.7
6,253,092	+		PFLU_RS28075	0.30	+0.7
6,253,093	-		PFLU_RS28075	0.30	+0.8
6,253,093	-		PFLU_RS28075	0.30	+0.4
6,253,093	-		PFLU_RS28075	0.30	+0.1
6,253,093	-		PFLU_RS28075	0.30	-2.7
6,253,093	-		PFLU_RS28075	0.30	-1.3
6,253,093	-		PFLU_RS28075	0.30	+0.5
6,253,131	+		PFLU_RS28075	0.42	+1.4
6,253,157	+		PFLU_RS28075	0.50	+0.2
6,253,157	+		PFLU_RS28075	0.50	+0.9
6,253,157	+		PFLU_RS28075	0.50	-0.6
6,253,157	+		PFLU_RS28075	0.50	-1.0
6,253,157	+		PFLU_RS28075	0.50	+0.2
6,253,157	+		PFLU_RS28075	0.50	-0.1
6,253,157	+		PFLU_RS28075	0.50	-0.4
6,253,157	+		PFLU_RS28075	0.50	-1.5
6,253,158	-		PFLU_RS28075	0.50	+0.5
6,253,158	-		PFLU_RS28075	0.50	+0.6
6,253,158	-		PFLU_RS28075	0.50	+0.4
6,253,158	-		PFLU_RS28075	0.50	-0.3
6,253,158	-		PFLU_RS28075	0.50	+3.0
6,253,158	-		PFLU_RS28075	0.50	-1.4
6,253,158	-		PFLU_RS28075	0.50	-0.2
6,253,158	-		PFLU_RS28075	0.50	-0.3
6,253,171	+		PFLU_RS28075	0.54	-0.8
6,253,237	+		PFLU_RS28075	0.74	-3.5
6,253,238	-		PFLU_RS28075	0.74	+0.2
6,253,370	+				-0.3
6,253,370	+				+0.0
6,253,371	-				+0.5
6,253,371	-				-0.9
6,253,426	+		PFLU_RS28080	0.18	+0.5
6,253,426	+		PFLU_RS28080	0.18	-0.8
6,253,426	+		PFLU_RS28080	0.18	-0.9
6,253,426	+		PFLU_RS28080	0.18	+0.1
6,253,426	+		PFLU_RS28080	0.18	-1.3
6,253,427	-		PFLU_RS28080	0.18	+1.4
6,253,427	-		PFLU_RS28080	0.18	+0.3
6,253,427	-		PFLU_RS28080	0.18	-0.2
6,253,499	-		PFLU_RS28080	0.50	+1.4
6,253,619	+				+0.1
6,253,619	+				-0.1
6,253,620	-				-1.4
6,253,620	-				+0.0
6,253,620	-				+0.4
6,253,620	-				-0.4