Experiment: SJC12_cps8_0.1MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2647 and BT2648 are separated by 12 nucleotides BT2648 and BT2649 overlap by 4 nucleotides BT2649 and BT2650 are separated by 97 nucleotides BT2650 and BT2651 are separated by 57 nucleotides
BT2647: BT2647 - hypothetical protein (NCBI ptt file), at 3,299,935 to 3,301,041
BT2647
BT2648: BT2648 - hypothetical protein (NCBI ptt file), at 3,301,054 to 3,301,755
BT2648
BT2649: BT2649 - ThiF family protein, ubiquitin-activating enzyme (NCBI ptt file), at 3,301,752 to 3,302,549
BT2649
BT2650: BT2650 - conserved hypothetical protein (NCBI ptt file), at 3,302,647 to 3,303,348
BT2650
BT2651: BT2651 - conserved hypothetical protein (NCBI ptt file), at 3,303,406 to 3,303,699
BT2651
Position (kb)
3301
3302
3303 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3300.769 kb on + strand, within BT2647 at 3300.770 kb on - strand, within BT2647 at 3300.770 kb on - strand, within BT2647 at 3300.787 kb on + strand, within BT2647 at 3300.788 kb on - strand, within BT2647 at 3300.910 kb on + strand, within BT2647 at 3301.039 kb on + strand at 3301.103 kb on + strand at 3301.213 kb on + strand, within BT2648 at 3301.213 kb on + strand, within BT2648 at 3301.327 kb on + strand, within BT2648 at 3301.331 kb on + strand, within BT2648 at 3301.331 kb on + strand, within BT2648 at 3301.332 kb on - strand, within BT2648 at 3301.332 kb on - strand, within BT2648 at 3301.332 kb on - strand, within BT2648 at 3301.332 kb on - strand at 3301.332 kb on - strand, within BT2648 at 3301.332 kb on - strand, within BT2648 at 3301.337 kb on - strand, within BT2648 at 3301.420 kb on + strand, within BT2648 at 3301.420 kb on + strand, within BT2648 at 3301.448 kb on + strand, within BT2648 at 3301.448 kb on + strand, within BT2648 at 3301.449 kb on - strand, within BT2648 at 3301.449 kb on - strand, within BT2648 at 3301.492 kb on + strand, within BT2648 at 3301.493 kb on - strand, within BT2648 at 3301.715 kb on + strand at 3301.745 kb on + strand at 3301.767 kb on + strand at 3301.780 kb on - strand at 3301.795 kb on - strand at 3301.903 kb on + strand, within BT2649 at 3301.904 kb on - strand, within BT2649 at 3301.904 kb on - strand, within BT2649 at 3301.948 kb on + strand, within BT2649 at 3301.948 kb on + strand, within BT2649 at 3301.949 kb on - strand, within BT2649 at 3302.066 kb on - strand, within BT2649 at 3302.260 kb on + strand, within BT2649 at 3302.260 kb on + strand, within BT2649 at 3302.261 kb on - strand, within BT2649 at 3302.261 kb on - strand, within BT2649 at 3302.282 kb on - strand, within BT2649 at 3302.350 kb on - strand, within BT2649 at 3302.422 kb on - strand, within BT2649 at 3302.434 kb on + strand, within BT2649 at 3302.461 kb on + strand, within BT2649 at 3302.462 kb on - strand, within BT2649 at 3302.505 kb on + strand at 3302.505 kb on + strand at 3302.505 kb on + strand at 3302.505 kb on + strand at 3302.505 kb on + strand at 3302.506 kb on - strand at 3302.506 kb on - strand at 3302.507 kb on + strand at 3302.508 kb on - strand at 3302.545 kb on + strand at 3302.548 kb on - strand at 3302.628 kb on + strand at 3302.629 kb on - strand at 3302.721 kb on - strand, within BT2650 at 3302.748 kb on - strand, within BT2650 at 3302.889 kb on + strand, within BT2650 at 3302.890 kb on - strand, within BT2650 at 3302.940 kb on + strand, within BT2650 at 3302.941 kb on - strand, within BT2650 at 3302.950 kb on + strand, within BT2650 at 3302.950 kb on + strand, within BT2650 at 3302.951 kb on - strand, within BT2650 at 3303.012 kb on + strand, within BT2650 at 3303.012 kb on + strand, within BT2650 at 3303.012 kb on + strand, within BT2650 at 3303.030 kb on + strand, within BT2650 at 3303.033 kb on - strand, within BT2650 at 3303.123 kb on + strand, within BT2650 at 3303.224 kb on + strand, within BT2650 at 3303.225 kb on - strand, within BT2650 at 3303.226 kb on + strand, within BT2650 at 3303.227 kb on - strand, within BT2650 at 3303.227 kb on - strand, within BT2650 at 3303.229 kb on - strand, within BT2650 at 3303.229 kb on - strand, within BT2650 at 3303.229 kb on - strand, within BT2650 at 3303.391 kb on + strand at 3303.394 kb on - strand at 3303.488 kb on + strand, within BT2651
Per-strain Table
Position Strand Gene LocusTag Fraction SJC12_cps8_0.1MOI remove 3,300,769 + BT2647 0.75 -0.6 3,300,770 - BT2647 0.75 -0.9 3,300,770 - BT2647 0.75 +0.3 3,300,787 + BT2647 0.77 -2.5 3,300,788 - BT2647 0.77 -2.4 3,300,910 + BT2647 0.88 +1.7 3,301,039 + +1.8 3,301,103 + -2.5 3,301,213 + BT2648 0.23 -1.2 3,301,213 + BT2648 0.23 -2.8 3,301,327 + BT2648 0.39 -2.2 3,301,331 + BT2648 0.39 -2.2 3,301,331 + BT2648 0.39 -0.0 3,301,332 - BT2648 0.40 -3.1 3,301,332 - BT2648 0.40 -2.4 3,301,332 - BT2648 0.40 -1.9 3,301,332 - -0.4 3,301,332 - BT2648 0.40 -2.4 3,301,332 - BT2648 0.40 +1.2 3,301,337 - BT2648 0.40 -3.6 3,301,420 + BT2648 0.52 -1.6 3,301,420 + BT2648 0.52 +0.2 3,301,448 + BT2648 0.56 -1.6 3,301,448 + BT2648 0.56 -1.6 3,301,449 - BT2648 0.56 -2.4 3,301,449 - BT2648 0.56 -0.6 3,301,492 + BT2648 0.62 -2.9 3,301,493 - BT2648 0.63 -2.4 3,301,715 + +0.3 3,301,745 + -1.0 3,301,767 + -2.8 3,301,780 - -2.5 3,301,795 - -0.6 3,301,903 + BT2649 0.19 -2.1 3,301,904 - BT2649 0.19 -0.0 3,301,904 - BT2649 0.19 +1.0 3,301,948 + BT2649 0.25 -1.4 3,301,948 + BT2649 0.25 -1.9 3,301,949 - BT2649 0.25 +1.5 3,302,066 - BT2649 0.39 -0.8 3,302,260 + BT2649 0.64 -2.5 3,302,260 + BT2649 0.64 -0.9 3,302,261 - BT2649 0.64 -0.6 3,302,261 - BT2649 0.64 -0.5 3,302,282 - BT2649 0.66 -2.6 3,302,350 - BT2649 0.75 -1.8 3,302,422 - BT2649 0.84 +0.6 3,302,434 + BT2649 0.85 +0.2 3,302,461 + BT2649 0.89 -0.2 3,302,462 - BT2649 0.89 -1.9 3,302,505 + -0.7 3,302,505 + +1.0 3,302,505 + -1.0 3,302,505 + -0.0 3,302,505 + -2.1 3,302,506 - +2.2 3,302,506 - +0.5 3,302,507 + -0.6 3,302,508 - +0.4 3,302,545 + -2.1 3,302,548 - -2.5 3,302,628 + -1.4 3,302,629 - +0.8 3,302,721 - BT2650 0.11 -0.0 3,302,748 - BT2650 0.14 -1.4 3,302,889 + BT2650 0.34 -1.0 3,302,890 - BT2650 0.35 -0.1 3,302,940 + BT2650 0.42 -2.8 3,302,941 - BT2650 0.42 -0.3 3,302,950 + BT2650 0.43 -1.8 3,302,950 + BT2650 0.43 -0.9 3,302,951 - BT2650 0.43 +2.3 3,303,012 + BT2650 0.52 -2.0 3,303,012 + BT2650 0.52 +1.8 3,303,012 + BT2650 0.52 +2.3 3,303,030 + BT2650 0.55 -2.2 3,303,033 - BT2650 0.55 +0.4 3,303,123 + BT2650 0.68 -2.2 3,303,224 + BT2650 0.82 -2.4 3,303,225 - BT2650 0.82 -1.5 3,303,226 + BT2650 0.82 -0.5 3,303,227 - BT2650 0.83 -2.4 3,303,227 - BT2650 0.83 -0.6 3,303,229 - BT2650 0.83 -0.8 3,303,229 - BT2650 0.83 +1.0 3,303,229 - BT2650 0.83 -0.0 3,303,391 + -3.3 3,303,394 - +0.5 3,303,488 + BT2651 0.28 -1.1
Or see this region's nucleotide sequence