Experiment: SJC12_cps8_0.1MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1726 and BT1727 are separated by 568 nucleotides BT1727 and BT1728 are separated by 9 nucleotides BT1728 and BT1729 are separated by 76 nucleotides
BT1726: BT1726 - integrase (NCBI ptt file), at 2,124,568 to 2,125,518
BT1726
BT1727: BT1727 - putative transmembrane sensor (NCBI ptt file), at 2,126,087 to 2,126,938
BT1727
BT1728: BT1728 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 2,126,948 to 2,127,538
BT1728
BT1729: BT1729 - peptide chain release factor 3 (RF-3) (NCBI ptt file), at 2,127,615 to 2,129,213
BT1729
Position (kb)
2126
2127 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2125.272 kb on - strand, within BT1726 at 2125.276 kb on - strand, within BT1726 at 2125.288 kb on - strand, within BT1726 at 2125.294 kb on - strand, within BT1726 at 2125.294 kb on - strand, within BT1726 at 2125.296 kb on - strand, within BT1726 at 2125.390 kb on - strand, within BT1726 at 2125.512 kb on + strand at 2125.523 kb on + strand at 2125.523 kb on + strand at 2125.531 kb on + strand at 2125.554 kb on + strand at 2125.572 kb on + strand at 2125.597 kb on - strand at 2125.602 kb on - strand at 2125.701 kb on - strand at 2125.701 kb on - strand at 2125.711 kb on - strand at 2125.713 kb on + strand at 2125.732 kb on + strand at 2125.797 kb on - strand at 2125.806 kb on - strand at 2125.818 kb on - strand at 2125.876 kb on + strand at 2125.880 kb on + strand at 2125.881 kb on - strand at 2125.906 kb on - strand at 2126.039 kb on - strand at 2126.040 kb on + strand at 2126.050 kb on - strand at 2126.093 kb on + strand at 2126.197 kb on - strand, within BT1727 at 2126.197 kb on - strand, within BT1727 at 2126.223 kb on - strand, within BT1727 at 2126.223 kb on - strand, within BT1727 at 2126.223 kb on - strand, within BT1727 at 2126.243 kb on - strand, within BT1727 at 2126.262 kb on - strand, within BT1727 at 2126.295 kb on - strand, within BT1727 at 2126.336 kb on - strand, within BT1727 at 2126.397 kb on - strand, within BT1727 at 2126.499 kb on - strand, within BT1727 at 2126.587 kb on - strand, within BT1727 at 2126.651 kb on + strand, within BT1727 at 2126.652 kb on - strand, within BT1727 at 2126.665 kb on + strand, within BT1727 at 2126.665 kb on + strand, within BT1727 at 2126.685 kb on + strand, within BT1727 at 2126.688 kb on + strand, within BT1727 at 2126.688 kb on + strand, within BT1727 at 2126.837 kb on + strand, within BT1727 at 2126.905 kb on + strand at 2126.908 kb on - strand at 2126.908 kb on - strand at 2126.908 kb on - strand at 2127.034 kb on + strand, within BT1728 at 2127.034 kb on + strand, within BT1728 at 2127.038 kb on - strand, within BT1728 at 2127.041 kb on - strand, within BT1728 at 2127.101 kb on - strand, within BT1728 at 2127.115 kb on - strand, within BT1728 at 2127.120 kb on - strand at 2127.170 kb on + strand, within BT1728 at 2127.212 kb on + strand, within BT1728 at 2127.212 kb on + strand, within BT1728 at 2127.213 kb on - strand, within BT1728 at 2127.213 kb on - strand, within BT1728 at 2127.213 kb on - strand, within BT1728 at 2127.214 kb on - strand, within BT1728 at 2127.219 kb on - strand, within BT1728 at 2127.235 kb on + strand, within BT1728 at 2127.280 kb on + strand, within BT1728 at 2127.379 kb on - strand, within BT1728 at 2127.385 kb on - strand, within BT1728 at 2127.421 kb on + strand, within BT1728 at 2127.422 kb on - strand, within BT1728 at 2127.532 kb on + strand at 2127.533 kb on - strand at 2127.617 kb on - strand at 2127.642 kb on - strand at 2127.680 kb on - strand at 2127.728 kb on + strand at 2127.736 kb on + strand at 2127.740 kb on + strand at 2127.799 kb on + strand, within BT1729 at 2127.836 kb on - strand, within BT1729 at 2127.836 kb on - strand, within BT1729 at 2127.900 kb on - strand, within BT1729
Per-strain Table
Position Strand Gene LocusTag Fraction SJC12_cps8_0.1MOI remove 2,125,272 - BT1726 0.74 -0.5 2,125,276 - BT1726 0.74 -2.5 2,125,288 - BT1726 0.76 -1.7 2,125,294 - BT1726 0.76 -2.3 2,125,294 - BT1726 0.76 +0.6 2,125,296 - BT1726 0.77 -2.9 2,125,390 - BT1726 0.86 -2.6 2,125,512 + -1.6 2,125,523 + -1.5 2,125,523 + -2.0 2,125,531 + -0.1 2,125,554 + -2.1 2,125,572 + +1.2 2,125,597 - +0.1 2,125,602 - -1.8 2,125,701 - -2.1 2,125,701 - +1.3 2,125,711 - -1.0 2,125,713 + -0.9 2,125,732 + -1.9 2,125,797 - -2.0 2,125,806 - -0.7 2,125,818 - -0.2 2,125,876 + +0.5 2,125,880 + +1.1 2,125,881 - -1.5 2,125,906 - -1.5 2,126,039 - -0.2 2,126,040 + -1.3 2,126,050 - -0.4 2,126,093 + -0.9 2,126,197 - BT1727 0.13 +1.8 2,126,197 - BT1727 0.13 -0.9 2,126,223 - BT1727 0.16 -0.3 2,126,223 - BT1727 0.16 -3.3 2,126,223 - BT1727 0.16 -0.4 2,126,243 - BT1727 0.18 +0.4 2,126,262 - BT1727 0.21 -1.9 2,126,295 - BT1727 0.24 +0.1 2,126,336 - BT1727 0.29 -2.3 2,126,397 - BT1727 0.36 -1.9 2,126,499 - BT1727 0.48 +1.1 2,126,587 - BT1727 0.59 -0.5 2,126,651 + BT1727 0.66 +1.1 2,126,652 - BT1727 0.66 -2.1 2,126,665 + BT1727 0.68 -0.1 2,126,665 + BT1727 0.68 -2.5 2,126,685 + BT1727 0.70 +1.1 2,126,688 + BT1727 0.71 -0.5 2,126,688 + BT1727 0.71 -3.9 2,126,837 + BT1727 0.88 +1.6 2,126,905 + -3.7 2,126,908 - -2.1 2,126,908 - -3.0 2,126,908 - +0.1 2,127,034 + BT1728 0.15 -0.7 2,127,034 + BT1728 0.15 +2.4 2,127,038 - BT1728 0.15 +0.2 2,127,041 - BT1728 0.16 -1.9 2,127,101 - BT1728 0.26 -1.9 2,127,115 - BT1728 0.28 +2.0 2,127,120 - +1.1 2,127,170 + BT1728 0.38 +0.2 2,127,212 + BT1728 0.45 -1.3 2,127,212 + BT1728 0.45 +0.5 2,127,213 - BT1728 0.45 +1.1 2,127,213 - BT1728 0.45 -3.6 2,127,213 - BT1728 0.45 -2.2 2,127,214 - BT1728 0.45 -0.5 2,127,219 - BT1728 0.46 +0.5 2,127,235 + BT1728 0.49 +0.3 2,127,280 + BT1728 0.56 -1.7 2,127,379 - BT1728 0.73 -0.5 2,127,385 - BT1728 0.74 -1.3 2,127,421 + BT1728 0.80 -2.1 2,127,422 - BT1728 0.80 -0.2 2,127,532 + -2.9 2,127,533 - -1.3 2,127,617 - -1.7 2,127,642 - +1.1 2,127,680 - -1.7 2,127,728 + -1.6 2,127,736 + -2.4 2,127,740 + -2.0 2,127,799 + BT1729 0.12 +0.1 2,127,836 - BT1729 0.14 -0.1 2,127,836 - BT1729 0.14 -2.0 2,127,900 - BT1729 0.18 -1.3
Or see this region's nucleotide sequence