Experiment: DAC22_cps8_0.1MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1077 and BT1078 are separated by 150 nucleotides BT1078 and BT1079 overlap by 4 nucleotides BT1079 and BT1080 are separated by 6 nucleotides BT1080 and BT1081 are separated by 36 nucleotides
BT1077: BT1077 - putative aspartate aminotransferase (NCBI ptt file), at 1,363,962 to 1,365,278
BT1077
BT1078: BT1078 - putative transcriptional regulator (NCBI ptt file), at 1,365,429 to 1,366,019
BT1078
BT1079: BT1079 - acetyltransferase (NCBI ptt file), at 1,366,016 to 1,366,555
BT1079
BT1080: BT1080 - hypothetical protein (NCBI ptt file), at 1,366,562 to 1,367,011
BT1080
BT1081: BT1081 - recombination protein recR (NCBI ptt file), at 1,367,048 to 1,367,665
BT1081
Position (kb)
1366
1367 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1365.034 kb on + strand, within BT1077 at 1365.132 kb on + strand, within BT1077 at 1365.133 kb on - strand, within BT1077 at 1365.198 kb on - strand at 1365.206 kb on + strand at 1365.287 kb on + strand at 1365.400 kb on - strand at 1365.400 kb on - strand at 1365.467 kb on + strand at 1365.623 kb on + strand, within BT1078 at 1365.639 kb on + strand, within BT1078 at 1365.640 kb on - strand, within BT1078 at 1365.640 kb on - strand, within BT1078 at 1365.661 kb on - strand, within BT1078 at 1365.686 kb on + strand, within BT1078 at 1365.686 kb on + strand, within BT1078 at 1365.704 kb on + strand, within BT1078 at 1365.704 kb on + strand, within BT1078 at 1365.704 kb on + strand, within BT1078 at 1365.705 kb on - strand, within BT1078 at 1365.787 kb on + strand, within BT1078 at 1365.788 kb on - strand, within BT1078 at 1365.938 kb on + strand, within BT1078 at 1365.964 kb on + strand at 1365.965 kb on - strand at 1365.975 kb on + strand at 1365.975 kb on + strand at 1365.976 kb on - strand at 1366.017 kb on + strand at 1366.200 kb on + strand, within BT1079 at 1366.571 kb on - strand at 1366.667 kb on - strand, within BT1080 at 1366.966 kb on - strand, within BT1080 at 1367.015 kb on - strand at 1367.151 kb on - strand, within BT1081 at 1367.188 kb on - strand, within BT1081 at 1367.286 kb on - strand, within BT1081 at 1367.474 kb on - strand, within BT1081 at 1367.496 kb on - strand, within BT1081
Per-strain Table
Position Strand Gene LocusTag Fraction DAC22_cps8_0.1MOI remove 1,365,034 + BT1077 0.81 +1.3 1,365,132 + BT1077 0.89 -1.0 1,365,133 - BT1077 0.89 +0.2 1,365,198 - +0.9 1,365,206 + -0.1 1,365,287 + -1.7 1,365,400 - -1.7 1,365,400 - -2.3 1,365,467 + -3.0 1,365,623 + BT1078 0.33 -0.4 1,365,639 + BT1078 0.36 +0.9 1,365,640 - BT1078 0.36 -1.9 1,365,640 - BT1078 0.36 -0.7 1,365,661 - BT1078 0.39 -0.1 1,365,686 + BT1078 0.43 -2.1 1,365,686 + BT1078 0.43 -0.7 1,365,704 + BT1078 0.47 +2.2 1,365,704 + BT1078 0.47 -1.1 1,365,704 + BT1078 0.47 -2.0 1,365,705 - BT1078 0.47 -1.5 1,365,787 + BT1078 0.61 +0.9 1,365,788 - BT1078 0.61 -0.8 1,365,938 + BT1078 0.86 -2.5 1,365,964 + +0.3 1,365,965 - -0.5 1,365,975 + -0.1 1,365,975 + +0.3 1,365,976 - +0.4 1,366,017 + -0.1 1,366,200 + BT1079 0.34 -0.1 1,366,571 - +0.9 1,366,667 - BT1080 0.23 -3.0 1,366,966 - BT1080 0.90 -2.0 1,367,015 - -0.7 1,367,151 - BT1081 0.17 -0.7 1,367,188 - BT1081 0.23 -0.5 1,367,286 - BT1081 0.39 +2.9 1,367,474 - BT1081 0.69 -1.1 1,367,496 - BT1081 0.72 +0.9
Or see this region's nucleotide sequence