Experiment: DAC16_cps7_0.1MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2644 and BT2645 are separated by 745 nucleotides BT2645 and BT2646 are separated by 21 nucleotides BT2646 and BT2647 are separated by 44 nucleotides
BT2644: BT2644 - DNA topoisomerase I (NCBI ptt file), at 3,296,059 to 3,297,819
BT2644
BT2645: BT2645 - hypothetical protein (NCBI ptt file), at 3,298,565 to 3,299,647
BT2645
BT2646: BT2646 - conserved hypothetical protein (NCBI ptt file), at 3,299,669 to 3,299,890
BT2646
BT2647: BT2647 - hypothetical protein (NCBI ptt file), at 3,299,935 to 3,301,041
BT2647
Position (kb)
3298
3299
3300 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3297.595 kb on + strand, within BT2644 at 3297.595 kb on + strand, within BT2644 at 3297.595 kb on + strand, within BT2644 at 3297.596 kb on - strand, within BT2644 at 3297.599 kb on + strand, within BT2644 at 3297.600 kb on - strand, within BT2644 at 3297.600 kb on - strand, within BT2644 at 3297.604 kb on + strand, within BT2644 at 3297.616 kb on + strand, within BT2644 at 3297.616 kb on + strand, within BT2644 at 3297.616 kb on + strand, within BT2644 at 3297.643 kb on + strand, within BT2644 at 3297.644 kb on - strand at 3297.647 kb on + strand at 3297.647 kb on + strand at 3297.647 kb on + strand at 3297.647 kb on + strand at 3297.648 kb on - strand at 3297.649 kb on + strand at 3297.649 kb on + strand at 3297.649 kb on + strand at 3297.649 kb on + strand at 3297.650 kb on - strand at 3297.650 kb on - strand at 3297.650 kb on - strand at 3297.786 kb on - strand at 3297.825 kb on - strand at 3297.825 kb on - strand at 3297.827 kb on - strand at 3297.827 kb on - strand at 3297.829 kb on - strand at 3297.829 kb on - strand at 3297.831 kb on - strand at 3297.831 kb on - strand at 3297.831 kb on - strand at 3297.831 kb on - strand at 3297.831 kb on - strand at 3297.873 kb on - strand at 3298.081 kb on - strand at 3298.081 kb on - strand at 3298.081 kb on - strand at 3298.081 kb on - strand at 3298.169 kb on - strand at 3298.169 kb on - strand at 3298.169 kb on - strand at 3298.206 kb on - strand at 3298.206 kb on - strand at 3298.226 kb on - strand at 3298.238 kb on - strand at 3298.313 kb on - strand at 3298.433 kb on - strand at 3298.464 kb on - strand at 3298.524 kb on - strand at 3298.543 kb on - strand at 3298.545 kb on - strand at 3298.660 kb on - strand at 3298.670 kb on - strand at 3298.670 kb on - strand at 3299.241 kb on + strand, within BT2645 at 3299.245 kb on - strand, within BT2645 at 3299.245 kb on - strand, within BT2645 at 3299.267 kb on + strand, within BT2645 at 3299.268 kb on - strand, within BT2645 at 3299.301 kb on - strand, within BT2645 at 3299.635 kb on + strand at 3299.636 kb on - strand at 3299.638 kb on - strand at 3299.638 kb on - strand at 3299.701 kb on - strand, within BT2646 at 3299.853 kb on + strand, within BT2646 at 3299.895 kb on - strand at 3299.956 kb on - strand at 3299.981 kb on - strand at 3300.096 kb on + strand, within BT2647 at 3300.190 kb on + strand, within BT2647 at 3300.284 kb on + strand, within BT2647 at 3300.328 kb on + strand, within BT2647 at 3300.329 kb on - strand, within BT2647 at 3300.367 kb on - strand, within BT2647 at 3300.426 kb on - strand, within BT2647 at 3300.511 kb on + strand, within BT2647 at 3300.533 kb on - strand, within BT2647
Per-strain Table
Position Strand Gene LocusTag Fraction DAC16_cps7_0.1MOI remove 3,297,595 + BT2644 0.87 -2.4 3,297,595 + BT2644 0.87 -0.8 3,297,595 + BT2644 0.87 +0.5 3,297,596 - BT2644 0.87 +1.0 3,297,599 + BT2644 0.87 -0.9 3,297,600 - BT2644 0.88 -1.1 3,297,600 - BT2644 0.88 -0.8 3,297,604 + BT2644 0.88 -0.5 3,297,616 + BT2644 0.88 -1.0 3,297,616 + BT2644 0.88 +0.5 3,297,616 + BT2644 0.88 -1.6 3,297,643 + BT2644 0.90 +1.0 3,297,644 - -0.2 3,297,647 + -1.7 3,297,647 + -0.3 3,297,647 + -0.3 3,297,647 + +0.3 3,297,648 - -1.8 3,297,649 + -1.4 3,297,649 + +0.2 3,297,649 + -0.7 3,297,649 + +1.9 3,297,650 - -0.4 3,297,650 - -1.5 3,297,650 - -0.6 3,297,786 - -0.6 3,297,825 - +0.0 3,297,825 - +0.4 3,297,827 - -2.6 3,297,827 - -0.6 3,297,829 - +0.8 3,297,829 - -0.7 3,297,831 - -0.6 3,297,831 - -1.3 3,297,831 - -2.5 3,297,831 - -2.9 3,297,831 - +0.2 3,297,873 - +0.6 3,298,081 - -1.3 3,298,081 - -0.6 3,298,081 - -2.2 3,298,081 - -1.2 3,298,169 - -0.5 3,298,169 - -2.0 3,298,169 - +1.5 3,298,206 - +1.0 3,298,206 - -2.8 3,298,226 - +0.3 3,298,238 - -1.4 3,298,313 - +0.2 3,298,433 - -0.9 3,298,464 - +0.0 3,298,524 - -2.3 3,298,543 - -0.9 3,298,545 - -2.4 3,298,660 - +1.6 3,298,670 - -1.1 3,298,670 - -0.6 3,299,241 + BT2645 0.62 -2.3 3,299,245 - BT2645 0.63 -0.1 3,299,245 - BT2645 0.63 -1.9 3,299,267 + BT2645 0.65 -0.8 3,299,268 - BT2645 0.65 +1.6 3,299,301 - BT2645 0.68 -2.6 3,299,635 + -0.6 3,299,636 - -0.8 3,299,638 - -0.7 3,299,638 - -0.6 3,299,701 - BT2646 0.14 +0.0 3,299,853 + BT2646 0.83 -0.6 3,299,895 - -0.3 3,299,956 - +0.0 3,299,981 - -1.1 3,300,096 + BT2647 0.15 -0.3 3,300,190 + BT2647 0.23 -1.6 3,300,284 + BT2647 0.32 -1.8 3,300,328 + BT2647 0.36 -1.3 3,300,329 - BT2647 0.36 -0.8 3,300,367 - BT2647 0.39 +0.0 3,300,426 - BT2647 0.44 +2.6 3,300,511 + BT2647 0.52 -0.4 3,300,533 - BT2647 0.54 -1.8
Or see this region's nucleotide sequence