Experiment: Carbon source D,L-Lactate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3244 and Pf6N2E2_3245 are separated by 324 nucleotides Pf6N2E2_3245 and Pf6N2E2_3246 are separated by 299 nucleotides
Pf6N2E2_3244 - Medium-chain-fatty-acid--CoA ligase (EC 6.2.1.-), at 3,752,127 to 3,753,809
_3244
Pf6N2E2_3245 - L-lysine permease, at 3,754,134 to 3,754,763
_3245
Pf6N2E2_3246 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 3,755,063 to 3,756,028
_3246
Position (kb)
3754
3755 Strain fitness (log2 ratio)
-2
-1
0
1 at 3753.212 kb on - strand, within Pf6N2E2_3244 at 3753.247 kb on + strand, within Pf6N2E2_3244 at 3753.247 kb on + strand, within Pf6N2E2_3244 at 3753.247 kb on + strand, within Pf6N2E2_3244 at 3753.247 kb on + strand, within Pf6N2E2_3244 at 3753.248 kb on - strand, within Pf6N2E2_3244 at 3753.248 kb on - strand, within Pf6N2E2_3244 at 3753.248 kb on - strand, within Pf6N2E2_3244 at 3753.248 kb on - strand, within Pf6N2E2_3244 at 3753.292 kb on + strand, within Pf6N2E2_3244 at 3753.293 kb on - strand, within Pf6N2E2_3244 at 3753.293 kb on - strand, within Pf6N2E2_3244 at 3753.293 kb on - strand, within Pf6N2E2_3244 at 3753.325 kb on + strand, within Pf6N2E2_3244 at 3753.325 kb on + strand, within Pf6N2E2_3244 at 3753.325 kb on + strand, within Pf6N2E2_3244 at 3753.325 kb on + strand, within Pf6N2E2_3244 at 3753.325 kb on + strand, within Pf6N2E2_3244 at 3753.325 kb on + strand, within Pf6N2E2_3244 at 3753.326 kb on - strand, within Pf6N2E2_3244 at 3753.424 kb on + strand, within Pf6N2E2_3244 at 3753.425 kb on - strand, within Pf6N2E2_3244 at 3753.425 kb on - strand, within Pf6N2E2_3244 at 3753.425 kb on - strand, within Pf6N2E2_3244 at 3753.425 kb on - strand, within Pf6N2E2_3244 at 3753.633 kb on + strand, within Pf6N2E2_3244 at 3753.633 kb on + strand, within Pf6N2E2_3244 at 3753.633 kb on + strand, within Pf6N2E2_3244 at 3753.633 kb on + strand, within Pf6N2E2_3244 at 3753.634 kb on - strand, within Pf6N2E2_3244 at 3753.634 kb on - strand, within Pf6N2E2_3244 at 3753.634 kb on - strand, within Pf6N2E2_3244 at 3753.634 kb on - strand, within Pf6N2E2_3244 at 3753.698 kb on - strand at 3753.764 kb on - strand at 3753.791 kb on + strand at 3753.809 kb on + strand at 3753.809 kb on + strand at 3753.809 kb on + strand at 3753.809 kb on + strand at 3753.809 kb on + strand at 3753.809 kb on + strand at 3753.810 kb on - strand at 3753.810 kb on - strand at 3753.810 kb on - strand at 3753.810 kb on - strand at 3753.810 kb on - strand at 3753.810 kb on - strand at 3753.810 kb on - strand at 3753.810 kb on - strand at 3753.857 kb on + strand at 3753.858 kb on - strand at 3753.858 kb on - strand at 3754.001 kb on + strand at 3754.002 kb on - strand at 3754.002 kb on - strand at 3754.002 kb on - strand at 3754.013 kb on + strand at 3754.297 kb on + strand, within Pf6N2E2_3245 at 3754.297 kb on + strand, within Pf6N2E2_3245 at 3754.298 kb on - strand, within Pf6N2E2_3245 at 3754.298 kb on - strand, within Pf6N2E2_3245 at 3754.434 kb on - strand, within Pf6N2E2_3245 at 3754.434 kb on - strand, within Pf6N2E2_3245 at 3754.510 kb on + strand, within Pf6N2E2_3245 at 3754.510 kb on + strand, within Pf6N2E2_3245 at 3754.510 kb on + strand, within Pf6N2E2_3245 at 3754.510 kb on + strand, within Pf6N2E2_3245 at 3754.510 kb on + strand, within Pf6N2E2_3245 at 3754.510 kb on + strand, within Pf6N2E2_3245 at 3754.511 kb on - strand, within Pf6N2E2_3245 at 3754.511 kb on - strand, within Pf6N2E2_3245 at 3754.511 kb on - strand, within Pf6N2E2_3245 at 3754.511 kb on - strand, within Pf6N2E2_3245 at 3754.511 kb on - strand, within Pf6N2E2_3245 at 3754.511 kb on - strand, within Pf6N2E2_3245 at 3754.519 kb on + strand, within Pf6N2E2_3245 at 3754.520 kb on - strand, within Pf6N2E2_3245 at 3754.759 kb on + strand at 3754.759 kb on + strand at 3754.760 kb on - strand at 3754.760 kb on - strand at 3754.823 kb on - strand at 3754.823 kb on - strand at 3754.869 kb on + strand at 3754.869 kb on + strand at 3754.870 kb on - strand at 3754.870 kb on - strand at 3754.870 kb on - strand at 3754.870 kb on - strand at 3754.870 kb on - strand at 3754.870 kb on - strand at 3754.877 kb on + strand at 3754.878 kb on - strand at 3754.942 kb on + strand at 3755.000 kb on + strand at 3755.000 kb on + strand at 3755.000 kb on + strand at 3755.000 kb on + strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.002 kb on + strand at 3755.028 kb on + strand at 3755.028 kb on + strand at 3755.028 kb on + strand at 3755.029 kb on - strand at 3755.055 kb on + strand at 3755.056 kb on - strand at 3755.056 kb on - strand at 3755.056 kb on - strand at 3755.056 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source D,L-Lactate 10 mM remove 3,753,212 - Pf6N2E2_3244 0.64 +1.4 3,753,247 + Pf6N2E2_3244 0.67 -1.8 3,753,247 + Pf6N2E2_3244 0.67 +0.7 3,753,247 + Pf6N2E2_3244 0.67 -0.4 3,753,247 + Pf6N2E2_3244 0.67 +0.4 3,753,248 - Pf6N2E2_3244 0.67 +0.7 3,753,248 - Pf6N2E2_3244 0.67 +1.6 3,753,248 - Pf6N2E2_3244 0.67 -0.6 3,753,248 - Pf6N2E2_3244 0.67 -0.2 3,753,292 + Pf6N2E2_3244 0.69 +0.1 3,753,293 - Pf6N2E2_3244 0.69 +0.4 3,753,293 - Pf6N2E2_3244 0.69 -0.2 3,753,293 - Pf6N2E2_3244 0.69 -0.4 3,753,325 + Pf6N2E2_3244 0.71 +1.4 3,753,325 + Pf6N2E2_3244 0.71 +1.4 3,753,325 + Pf6N2E2_3244 0.71 +0.9 3,753,325 + Pf6N2E2_3244 0.71 -0.6 3,753,325 + Pf6N2E2_3244 0.71 -0.6 3,753,325 + Pf6N2E2_3244 0.71 -0.4 3,753,326 - Pf6N2E2_3244 0.71 +0.6 3,753,424 + Pf6N2E2_3244 0.77 +0.0 3,753,425 - Pf6N2E2_3244 0.77 -0.2 3,753,425 - Pf6N2E2_3244 0.77 -0.6 3,753,425 - Pf6N2E2_3244 0.77 +0.0 3,753,425 - Pf6N2E2_3244 0.77 +0.1 3,753,633 + Pf6N2E2_3244 0.89 +0.4 3,753,633 + Pf6N2E2_3244 0.89 +0.2 3,753,633 + Pf6N2E2_3244 0.89 -1.0 3,753,633 + Pf6N2E2_3244 0.89 -0.6 3,753,634 - Pf6N2E2_3244 0.90 -1.2 3,753,634 - Pf6N2E2_3244 0.90 -0.2 3,753,634 - Pf6N2E2_3244 0.90 +1.1 3,753,634 - Pf6N2E2_3244 0.90 +0.3 3,753,698 - -0.8 3,753,764 - +1.4 3,753,791 + -0.2 3,753,809 + +0.6 3,753,809 + +1.4 3,753,809 + +0.4 3,753,809 + +0.5 3,753,809 + +0.4 3,753,809 + +0.4 3,753,810 - +0.4 3,753,810 - +1.4 3,753,810 - +0.1 3,753,810 - -0.6 3,753,810 - +1.0 3,753,810 - -0.6 3,753,810 - -0.5 3,753,810 - -0.0 3,753,857 + +0.4 3,753,858 - +0.4 3,753,858 - +0.0 3,754,001 + -0.2 3,754,002 - +1.4 3,754,002 - -1.8 3,754,002 - +0.4 3,754,013 + +1.4 3,754,297 + Pf6N2E2_3245 0.26 +0.9 3,754,297 + Pf6N2E2_3245 0.26 -0.2 3,754,298 - Pf6N2E2_3245 0.26 -0.6 3,754,298 - Pf6N2E2_3245 0.26 -2.2 3,754,434 - Pf6N2E2_3245 0.48 -0.9 3,754,434 - Pf6N2E2_3245 0.48 -0.9 3,754,510 + Pf6N2E2_3245 0.60 -0.3 3,754,510 + Pf6N2E2_3245 0.60 +0.4 3,754,510 + Pf6N2E2_3245 0.60 -0.8 3,754,510 + Pf6N2E2_3245 0.60 -0.1 3,754,510 + Pf6N2E2_3245 0.60 -0.9 3,754,510 + Pf6N2E2_3245 0.60 -0.4 3,754,511 - Pf6N2E2_3245 0.60 -1.2 3,754,511 - Pf6N2E2_3245 0.60 -0.7 3,754,511 - Pf6N2E2_3245 0.60 +0.2 3,754,511 - Pf6N2E2_3245 0.60 -0.1 3,754,511 - Pf6N2E2_3245 0.60 +1.0 3,754,511 - Pf6N2E2_3245 0.60 -1.6 3,754,519 + Pf6N2E2_3245 0.61 +0.7 3,754,520 - Pf6N2E2_3245 0.61 +1.4 3,754,759 + -2.4 3,754,759 + -0.9 3,754,760 - -0.1 3,754,760 - -0.1 3,754,823 - -0.2 3,754,823 - +0.4 3,754,869 + -0.8 3,754,869 + -0.3 3,754,870 - -0.7 3,754,870 - +0.4 3,754,870 - +0.0 3,754,870 - +1.1 3,754,870 - -0.5 3,754,870 - +0.5 3,754,877 + +0.4 3,754,878 - +1.0 3,754,942 + -0.6 3,755,000 + +0.2 3,755,000 + -2.0 3,755,000 + -0.3 3,755,000 + -0.6 3,755,001 - -0.3 3,755,001 - +0.4 3,755,001 - +1.4 3,755,001 - +0.2 3,755,001 - +0.4 3,755,001 - +0.7 3,755,001 - +0.6 3,755,001 - +0.4 3,755,001 - +0.3 3,755,001 - -0.9 3,755,001 - -1.0 3,755,002 + +0.8 3,755,028 + -0.4 3,755,028 + +1.4 3,755,028 + -0.1 3,755,029 - +0.4 3,755,055 + -0.1 3,755,056 - +0.6 3,755,056 - -1.4 3,755,056 - +0.3 3,755,056 - +0.5
Or see this region's nucleotide sequence