Experiment: Carbon source D,L-Lactate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1412 and Pf6N2E2_1413 are separated by 53 nucleotides Pf6N2E2_1413 and Pf6N2E2_1414 are separated by 158 nucleotides Pf6N2E2_1414 and Pf6N2E2_1415 overlap by 18 nucleotides Pf6N2E2_1415 and Pf6N2E2_1416 are separated by 300 nucleotides Pf6N2E2_1416 and Pf6N2E2_1417 are separated by 90 nucleotides Pf6N2E2_1417 and Pf6N2E2_1418 are separated by 174 nucleotides
Pf6N2E2_1412 - hypothetical protein, at 1,657,728 to 1,658,063
_1412
Pf6N2E2_1413 - putative membrane protein, at 1,658,117 to 1,658,560
_1413
Pf6N2E2_1414 - hypothetical protein, at 1,658,719 to 1,658,901
_1414
Pf6N2E2_1415 - mannuronan C-5-epimerase, putative, at 1,658,884 to 1,664,496
_1415
Pf6N2E2_1416 - Type I secretion outer membrane protein, TolC precursor, at 1,664,797 to 1,665,003
_1416
Pf6N2E2_1417 - Type I secretion outer membrane protein, TolC precursor, at 1,665,094 to 1,665,249
_1417
Pf6N2E2_1418 - Type I secretion outer membrane protein, TolC precursor, at 1,665,424 to 1,665,579
_1418
Position (kb)
1658
1659
1660
1661
1662
1663
1664
1665 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1657.935 kb on - strand, within Pf6N2E2_1412 at 1657.935 kb on - strand, within Pf6N2E2_1412 at 1657.935 kb on - strand, within Pf6N2E2_1412 at 1657.935 kb on - strand, within Pf6N2E2_1412 at 1657.941 kb on - strand, within Pf6N2E2_1412 at 1658.130 kb on + strand at 1658.130 kb on + strand at 1658.130 kb on + strand at 1658.131 kb on - strand at 1658.131 kb on - strand at 1658.131 kb on - strand at 1658.131 kb on - strand at 1658.683 kb on - strand at 1658.817 kb on + strand, within Pf6N2E2_1414 at 1658.995 kb on + strand at 1658.995 kb on + strand at 1658.995 kb on + strand at 1658.995 kb on + strand at 1658.995 kb on + strand at 1658.995 kb on + strand at 1658.995 kb on + strand at 1658.996 kb on - strand at 1658.996 kb on - strand at 1658.996 kb on - strand at 1658.996 kb on - strand at 1658.996 kb on - strand at 1659.014 kb on - strand at 1659.014 kb on - strand at 1659.073 kb on + strand at 1659.073 kb on + strand at 1659.161 kb on - strand at 1659.616 kb on + strand, within Pf6N2E2_1415 at 1659.616 kb on + strand, within Pf6N2E2_1415 at 1659.617 kb on - strand, within Pf6N2E2_1415 at 1659.833 kb on - strand, within Pf6N2E2_1415 at 1659.833 kb on - strand, within Pf6N2E2_1415 at 1659.833 kb on - strand, within Pf6N2E2_1415 at 1659.833 kb on - strand, within Pf6N2E2_1415 at 1659.833 kb on - strand, within Pf6N2E2_1415 at 1659.895 kb on + strand, within Pf6N2E2_1415 at 1659.895 kb on + strand, within Pf6N2E2_1415 at 1659.896 kb on - strand, within Pf6N2E2_1415 at 1659.988 kb on + strand, within Pf6N2E2_1415 at 1659.988 kb on + strand, within Pf6N2E2_1415 at 1659.989 kb on - strand at 1660.003 kb on + strand, within Pf6N2E2_1415 at 1660.003 kb on + strand, within Pf6N2E2_1415 at 1660.003 kb on + strand, within Pf6N2E2_1415 at 1660.004 kb on - strand, within Pf6N2E2_1415 at 1660.004 kb on - strand, within Pf6N2E2_1415 at 1660.004 kb on - strand, within Pf6N2E2_1415 at 1660.110 kb on + strand, within Pf6N2E2_1415 at 1660.375 kb on + strand, within Pf6N2E2_1415 at 1660.375 kb on + strand, within Pf6N2E2_1415 at 1660.375 kb on + strand, within Pf6N2E2_1415 at 1660.376 kb on - strand, within Pf6N2E2_1415 at 1660.376 kb on - strand, within Pf6N2E2_1415 at 1660.376 kb on - strand, within Pf6N2E2_1415 at 1660.388 kb on - strand, within Pf6N2E2_1415 at 1660.681 kb on + strand, within Pf6N2E2_1415 at 1660.682 kb on - strand, within Pf6N2E2_1415 at 1660.801 kb on + strand, within Pf6N2E2_1415 at 1660.802 kb on - strand, within Pf6N2E2_1415 at 1660.802 kb on - strand, within Pf6N2E2_1415 at 1660.834 kb on + strand, within Pf6N2E2_1415 at 1660.834 kb on + strand, within Pf6N2E2_1415 at 1660.835 kb on - strand, within Pf6N2E2_1415 at 1660.835 kb on - strand, within Pf6N2E2_1415 at 1660.835 kb on - strand, within Pf6N2E2_1415 at 1660.835 kb on - strand, within Pf6N2E2_1415 at 1660.835 kb on - strand, within Pf6N2E2_1415 at 1660.835 kb on - strand, within Pf6N2E2_1415 at 1660.897 kb on + strand, within Pf6N2E2_1415 at 1660.898 kb on - strand, within Pf6N2E2_1415 at 1660.898 kb on - strand, within Pf6N2E2_1415 at 1660.898 kb on - strand, within Pf6N2E2_1415 at 1661.173 kb on + strand, within Pf6N2E2_1415 at 1661.174 kb on - strand, within Pf6N2E2_1415 at 1661.174 kb on - strand, within Pf6N2E2_1415 at 1661.174 kb on - strand, within Pf6N2E2_1415 at 1661.229 kb on + strand, within Pf6N2E2_1415 at 1661.230 kb on - strand, within Pf6N2E2_1415 at 1661.435 kb on - strand, within Pf6N2E2_1415 at 1661.539 kb on + strand, within Pf6N2E2_1415 at 1661.540 kb on - strand, within Pf6N2E2_1415 at 1661.540 kb on - strand, within Pf6N2E2_1415 at 1661.540 kb on - strand, within Pf6N2E2_1415 at 1661.542 kb on - strand, within Pf6N2E2_1415 at 1661.584 kb on + strand, within Pf6N2E2_1415 at 1661.584 kb on + strand, within Pf6N2E2_1415 at 1661.584 kb on + strand, within Pf6N2E2_1415 at 1661.585 kb on - strand, within Pf6N2E2_1415 at 1661.608 kb on + strand, within Pf6N2E2_1415 at 1661.609 kb on - strand, within Pf6N2E2_1415 at 1661.845 kb on + strand, within Pf6N2E2_1415 at 1661.846 kb on - strand, within Pf6N2E2_1415 at 1661.846 kb on - strand, within Pf6N2E2_1415 at 1661.951 kb on - strand, within Pf6N2E2_1415 at 1661.951 kb on - strand, within Pf6N2E2_1415 at 1661.951 kb on - strand, within Pf6N2E2_1415 at 1661.957 kb on + strand, within Pf6N2E2_1415 at 1661.957 kb on + strand, within Pf6N2E2_1415 at 1661.957 kb on + strand, within Pf6N2E2_1415 at 1661.958 kb on - strand, within Pf6N2E2_1415 at 1662.117 kb on + strand, within Pf6N2E2_1415 at 1662.118 kb on - strand, within Pf6N2E2_1415 at 1662.239 kb on - strand, within Pf6N2E2_1415 at 1662.358 kb on + strand, within Pf6N2E2_1415 at 1662.358 kb on + strand, within Pf6N2E2_1415 at 1662.358 kb on + strand, within Pf6N2E2_1415 at 1662.358 kb on + strand, within Pf6N2E2_1415 at 1662.359 kb on - strand, within Pf6N2E2_1415 at 1662.359 kb on - strand, within Pf6N2E2_1415 at 1662.359 kb on - strand, within Pf6N2E2_1415 at 1662.359 kb on - strand, within Pf6N2E2_1415 at 1662.359 kb on - strand, within Pf6N2E2_1415 at 1662.359 kb on - strand, within Pf6N2E2_1415 at 1662.359 kb on - strand, within Pf6N2E2_1415 at 1662.359 kb on - strand, within Pf6N2E2_1415 at 1662.392 kb on - strand, within Pf6N2E2_1415 at 1662.392 kb on - strand, within Pf6N2E2_1415 at 1662.392 kb on - strand, within Pf6N2E2_1415 at 1662.616 kb on + strand, within Pf6N2E2_1415 at 1662.617 kb on - strand, within Pf6N2E2_1415 at 1662.625 kb on + strand, within Pf6N2E2_1415 at 1662.625 kb on + strand, within Pf6N2E2_1415 at 1662.924 kb on + strand, within Pf6N2E2_1415 at 1662.952 kb on + strand, within Pf6N2E2_1415 at 1662.952 kb on + strand, within Pf6N2E2_1415 at 1663.141 kb on + strand, within Pf6N2E2_1415 at 1663.142 kb on - strand, within Pf6N2E2_1415 at 1663.249 kb on + strand, within Pf6N2E2_1415 at 1663.250 kb on - strand, within Pf6N2E2_1415 at 1663.392 kb on + strand, within Pf6N2E2_1415 at 1663.392 kb on + strand, within Pf6N2E2_1415 at 1663.450 kb on + strand, within Pf6N2E2_1415 at 1663.450 kb on + strand, within Pf6N2E2_1415 at 1663.450 kb on + strand, within Pf6N2E2_1415 at 1663.451 kb on - strand, within Pf6N2E2_1415 at 1663.451 kb on - strand, within Pf6N2E2_1415 at 1663.451 kb on - strand, within Pf6N2E2_1415 at 1663.451 kb on - strand, within Pf6N2E2_1415 at 1663.451 kb on - strand, within Pf6N2E2_1415 at 1663.699 kb on + strand, within Pf6N2E2_1415 at 1663.699 kb on + strand, within Pf6N2E2_1415 at 1663.699 kb on + strand, within Pf6N2E2_1415 at 1663.699 kb on + strand, within Pf6N2E2_1415 at 1663.699 kb on + strand, within Pf6N2E2_1415 at 1663.699 kb on + strand, within Pf6N2E2_1415 at 1663.699 kb on + strand, within Pf6N2E2_1415 at 1663.699 kb on + strand, within Pf6N2E2_1415 at 1663.700 kb on - strand, within Pf6N2E2_1415 at 1663.700 kb on - strand, within Pf6N2E2_1415 at 1663.700 kb on - strand, within Pf6N2E2_1415 at 1663.700 kb on - strand, within Pf6N2E2_1415 at 1663.792 kb on + strand, within Pf6N2E2_1415 at 1663.792 kb on + strand, within Pf6N2E2_1415 at 1663.792 kb on + strand, within Pf6N2E2_1415 at 1663.792 kb on + strand, within Pf6N2E2_1415 at 1663.794 kb on + strand, within Pf6N2E2_1415 at 1663.794 kb on + strand, within Pf6N2E2_1415 at 1663.795 kb on - strand, within Pf6N2E2_1415 at 1663.795 kb on - strand, within Pf6N2E2_1415 at 1663.795 kb on - strand, within Pf6N2E2_1415 at 1663.822 kb on + strand, within Pf6N2E2_1415 at 1663.823 kb on - strand, within Pf6N2E2_1415 at 1663.872 kb on + strand, within Pf6N2E2_1415 at 1663.873 kb on - strand, within Pf6N2E2_1415 at 1663.873 kb on - strand, within Pf6N2E2_1415 at 1664.197 kb on + strand at 1664.197 kb on + strand at 1664.197 kb on + strand at 1664.198 kb on - strand at 1664.471 kb on + strand at 1664.471 kb on + strand at 1664.472 kb on - strand at 1664.523 kb on + strand at 1664.523 kb on + strand at 1664.524 kb on - strand at 1664.527 kb on + strand at 1664.528 kb on - strand at 1664.999 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source D,L-Lactate 10 mM remove 1,657,935 - Pf6N2E2_1412 0.62 -0.4 1,657,935 - Pf6N2E2_1412 0.62 -0.2 1,657,935 - Pf6N2E2_1412 0.62 +0.5 1,657,935 - Pf6N2E2_1412 0.62 +0.1 1,657,941 - Pf6N2E2_1412 0.63 -2.2 1,658,130 + +1.0 1,658,130 + +0.5 1,658,130 + -0.1 1,658,131 - -0.5 1,658,131 - -0.4 1,658,131 - -0.4 1,658,131 - +0.6 1,658,683 - -0.2 1,658,817 + Pf6N2E2_1414 0.54 +0.6 1,658,995 + +1.2 1,658,995 + -0.2 1,658,995 + +1.6 1,658,995 + +0.6 1,658,995 + -0.1 1,658,995 + -0.0 1,658,995 + -1.0 1,658,996 - +0.2 1,658,996 - +0.2 1,658,996 - -1.4 1,658,996 - -1.7 1,658,996 - -1.0 1,659,014 - +0.6 1,659,014 - -0.4 1,659,073 + +0.6 1,659,073 + -2.4 1,659,161 - +0.6 1,659,616 + Pf6N2E2_1415 0.13 +0.0 1,659,616 + Pf6N2E2_1415 0.13 +1.2 1,659,617 - Pf6N2E2_1415 0.13 -1.0 1,659,833 - Pf6N2E2_1415 0.17 +0.2 1,659,833 - Pf6N2E2_1415 0.17 -0.9 1,659,833 - Pf6N2E2_1415 0.17 -1.4 1,659,833 - Pf6N2E2_1415 0.17 -0.1 1,659,833 - Pf6N2E2_1415 0.17 -0.0 1,659,895 + Pf6N2E2_1415 0.18 -0.2 1,659,895 + Pf6N2E2_1415 0.18 +0.9 1,659,896 - Pf6N2E2_1415 0.18 -0.0 1,659,988 + Pf6N2E2_1415 0.20 -1.4 1,659,988 + Pf6N2E2_1415 0.20 +0.6 1,659,989 - +0.6 1,660,003 + Pf6N2E2_1415 0.20 -0.9 1,660,003 + Pf6N2E2_1415 0.20 -1.0 1,660,003 + Pf6N2E2_1415 0.20 -1.4 1,660,004 - Pf6N2E2_1415 0.20 +0.4 1,660,004 - Pf6N2E2_1415 0.20 -0.3 1,660,004 - Pf6N2E2_1415 0.20 -1.5 1,660,110 + Pf6N2E2_1415 0.22 +0.6 1,660,375 + Pf6N2E2_1415 0.27 -1.4 1,660,375 + Pf6N2E2_1415 0.27 +0.6 1,660,375 + Pf6N2E2_1415 0.27 +2.0 1,660,376 - Pf6N2E2_1415 0.27 +0.6 1,660,376 - Pf6N2E2_1415 0.27 +1.4 1,660,376 - Pf6N2E2_1415 0.27 -1.2 1,660,388 - Pf6N2E2_1415 0.27 +0.0 1,660,681 + Pf6N2E2_1415 0.32 -0.4 1,660,682 - Pf6N2E2_1415 0.32 +0.3 1,660,801 + Pf6N2E2_1415 0.34 +0.6 1,660,802 - Pf6N2E2_1415 0.34 +0.2 1,660,802 - Pf6N2E2_1415 0.34 -1.4 1,660,834 + Pf6N2E2_1415 0.35 +0.6 1,660,834 + Pf6N2E2_1415 0.35 -0.4 1,660,835 - Pf6N2E2_1415 0.35 +0.1 1,660,835 - Pf6N2E2_1415 0.35 +0.6 1,660,835 - Pf6N2E2_1415 0.35 -0.6 1,660,835 - Pf6N2E2_1415 0.35 +0.6 1,660,835 - Pf6N2E2_1415 0.35 -0.5 1,660,835 - Pf6N2E2_1415 0.35 -0.4 1,660,897 + Pf6N2E2_1415 0.36 +1.4 1,660,898 - Pf6N2E2_1415 0.36 +0.4 1,660,898 - Pf6N2E2_1415 0.36 -0.4 1,660,898 - Pf6N2E2_1415 0.36 -0.5 1,661,173 + Pf6N2E2_1415 0.41 -0.8 1,661,174 - Pf6N2E2_1415 0.41 -0.2 1,661,174 - Pf6N2E2_1415 0.41 -1.2 1,661,174 - Pf6N2E2_1415 0.41 +0.8 1,661,229 + Pf6N2E2_1415 0.42 -1.7 1,661,230 - Pf6N2E2_1415 0.42 +0.0 1,661,435 - Pf6N2E2_1415 0.45 +0.6 1,661,539 + Pf6N2E2_1415 0.47 +0.6 1,661,540 - Pf6N2E2_1415 0.47 -0.1 1,661,540 - Pf6N2E2_1415 0.47 +0.2 1,661,540 - Pf6N2E2_1415 0.47 -1.0 1,661,542 - Pf6N2E2_1415 0.47 -1.7 1,661,584 + Pf6N2E2_1415 0.48 +0.3 1,661,584 + Pf6N2E2_1415 0.48 +0.6 1,661,584 + Pf6N2E2_1415 0.48 +0.6 1,661,585 - Pf6N2E2_1415 0.48 -0.4 1,661,608 + Pf6N2E2_1415 0.49 -0.4 1,661,609 - Pf6N2E2_1415 0.49 +1.0 1,661,845 + Pf6N2E2_1415 0.53 +0.6 1,661,846 - Pf6N2E2_1415 0.53 +0.0 1,661,846 - Pf6N2E2_1415 0.53 -0.0 1,661,951 - Pf6N2E2_1415 0.55 +1.4 1,661,951 - Pf6N2E2_1415 0.55 +0.3 1,661,951 - Pf6N2E2_1415 0.55 +0.4 1,661,957 + Pf6N2E2_1415 0.55 -0.4 1,661,957 + Pf6N2E2_1415 0.55 -0.6 1,661,957 + Pf6N2E2_1415 0.55 -0.4 1,661,958 - Pf6N2E2_1415 0.55 +0.2 1,662,117 + Pf6N2E2_1415 0.58 -0.8 1,662,118 - Pf6N2E2_1415 0.58 +0.1 1,662,239 - Pf6N2E2_1415 0.60 +0.4 1,662,358 + Pf6N2E2_1415 0.62 +0.3 1,662,358 + Pf6N2E2_1415 0.62 +0.6 1,662,358 + Pf6N2E2_1415 0.62 +0.8 1,662,358 + Pf6N2E2_1415 0.62 +0.4 1,662,359 - Pf6N2E2_1415 0.62 +1.4 1,662,359 - Pf6N2E2_1415 0.62 +0.3 1,662,359 - Pf6N2E2_1415 0.62 +1.1 1,662,359 - Pf6N2E2_1415 0.62 -0.4 1,662,359 - Pf6N2E2_1415 0.62 -0.5 1,662,359 - Pf6N2E2_1415 0.62 +0.6 1,662,359 - Pf6N2E2_1415 0.62 -1.4 1,662,359 - Pf6N2E2_1415 0.62 -1.0 1,662,392 - Pf6N2E2_1415 0.62 +0.6 1,662,392 - Pf6N2E2_1415 0.62 +0.0 1,662,392 - Pf6N2E2_1415 0.62 +0.1 1,662,616 + Pf6N2E2_1415 0.66 +0.6 1,662,617 - Pf6N2E2_1415 0.67 +0.4 1,662,625 + Pf6N2E2_1415 0.67 +0.3 1,662,625 + Pf6N2E2_1415 0.67 -0.3 1,662,924 + Pf6N2E2_1415 0.72 +0.3 1,662,952 + Pf6N2E2_1415 0.72 +0.4 1,662,952 + Pf6N2E2_1415 0.72 +1.4 1,663,141 + Pf6N2E2_1415 0.76 -1.0 1,663,142 - Pf6N2E2_1415 0.76 -1.0 1,663,249 + Pf6N2E2_1415 0.78 +0.6 1,663,250 - Pf6N2E2_1415 0.78 +1.0 1,663,392 + Pf6N2E2_1415 0.80 +1.4 1,663,392 + Pf6N2E2_1415 0.80 -1.0 1,663,450 + Pf6N2E2_1415 0.81 +1.1 1,663,450 + Pf6N2E2_1415 0.81 +0.6 1,663,450 + Pf6N2E2_1415 0.81 -0.4 1,663,451 - Pf6N2E2_1415 0.81 -1.2 1,663,451 - Pf6N2E2_1415 0.81 +0.0 1,663,451 - Pf6N2E2_1415 0.81 -2.2 1,663,451 - Pf6N2E2_1415 0.81 -1.7 1,663,451 - Pf6N2E2_1415 0.81 +0.7 1,663,699 + Pf6N2E2_1415 0.86 -1.0 1,663,699 + Pf6N2E2_1415 0.86 -0.0 1,663,699 + Pf6N2E2_1415 0.86 -1.0 1,663,699 + Pf6N2E2_1415 0.86 +0.6 1,663,699 + Pf6N2E2_1415 0.86 +0.1 1,663,699 + Pf6N2E2_1415 0.86 +1.6 1,663,699 + Pf6N2E2_1415 0.86 +0.3 1,663,699 + Pf6N2E2_1415 0.86 +1.2 1,663,700 - Pf6N2E2_1415 0.86 -1.4 1,663,700 - Pf6N2E2_1415 0.86 -0.4 1,663,700 - Pf6N2E2_1415 0.86 +1.6 1,663,700 - Pf6N2E2_1415 0.86 -1.0 1,663,792 + Pf6N2E2_1415 0.87 +0.6 1,663,792 + Pf6N2E2_1415 0.87 +0.6 1,663,792 + Pf6N2E2_1415 0.87 +0.6 1,663,792 + Pf6N2E2_1415 0.87 +0.6 1,663,794 + Pf6N2E2_1415 0.87 -0.4 1,663,794 + Pf6N2E2_1415 0.87 -1.0 1,663,795 - Pf6N2E2_1415 0.87 +0.6 1,663,795 - Pf6N2E2_1415 0.87 -1.4 1,663,795 - Pf6N2E2_1415 0.87 +0.3 1,663,822 + Pf6N2E2_1415 0.88 -0.4 1,663,823 - Pf6N2E2_1415 0.88 +0.8 1,663,872 + Pf6N2E2_1415 0.89 +0.6 1,663,873 - Pf6N2E2_1415 0.89 -0.1 1,663,873 - Pf6N2E2_1415 0.89 +0.8 1,664,197 + +0.7 1,664,197 + +0.6 1,664,197 + +0.6 1,664,198 - -0.7 1,664,471 + +0.1 1,664,471 + -0.9 1,664,472 - -0.9 1,664,523 + +0.7 1,664,523 + +0.6 1,664,524 - +0.6 1,664,527 + +0.6 1,664,528 - -0.1 1,664,999 - -0.3
Or see this region's nucleotide sequence