Experiment: Carbon source Sucrose 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1073 and Pf6N2E2_1074 are separated by 229 nucleotides Pf6N2E2_1074 and Pf6N2E2_1075 are separated by 20 nucleotides Pf6N2E2_1075 and Pf6N2E2_1076 overlap by 4 nucleotides
Pf6N2E2_1073 - Glycogen debranching enzyme (EC 3.2.1.-), at 1,251,877 to 1,254,036
_1073
Pf6N2E2_1074 - FIG00953356: hypothetical protein, at 1,254,266 to 1,254,619
_1074
Pf6N2E2_1075 - Malto-oligosyltrehalose synthase (EC 5.4.99.15), at 1,254,640 to 1,257,426
_1075
Pf6N2E2_1076 - 4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25), at 1,257,423 to 1,259,501
_1076
Position (kb)
1254
1255
1256
1257
1258 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1253.765 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.781 kb on - strand, within Pf6N2E2_1073 at 1253.781 kb on - strand, within Pf6N2E2_1073 at 1253.781 kb on - strand, within Pf6N2E2_1073 at 1253.819 kb on + strand, within Pf6N2E2_1073 at 1253.834 kb on + strand at 1253.834 kb on + strand at 1254.036 kb on + strand at 1254.036 kb on + strand at 1254.036 kb on + strand at 1254.036 kb on + strand at 1254.036 kb on + strand at 1254.037 kb on - strand at 1254.037 kb on - strand at 1254.132 kb on + strand at 1254.133 kb on - strand at 1254.133 kb on - strand at 1254.560 kb on - strand, within Pf6N2E2_1074 at 1254.583 kb on - strand, within Pf6N2E2_1074 at 1254.850 kb on + strand at 1254.850 kb on + strand at 1254.851 kb on - strand at 1254.851 kb on - strand at 1254.851 kb on - strand at 1254.955 kb on + strand, within Pf6N2E2_1075 at 1254.955 kb on + strand, within Pf6N2E2_1075 at 1254.956 kb on - strand, within Pf6N2E2_1075 at 1255.149 kb on - strand, within Pf6N2E2_1075 at 1255.149 kb on - strand, within Pf6N2E2_1075 at 1255.316 kb on + strand, within Pf6N2E2_1075 at 1255.556 kb on + strand, within Pf6N2E2_1075 at 1255.557 kb on - strand, within Pf6N2E2_1075 at 1255.721 kb on + strand, within Pf6N2E2_1075 at 1256.006 kb on + strand, within Pf6N2E2_1075 at 1256.006 kb on + strand, within Pf6N2E2_1075 at 1256.006 kb on + strand, within Pf6N2E2_1075 at 1256.006 kb on + strand, within Pf6N2E2_1075 at 1256.006 kb on + strand, within Pf6N2E2_1075 at 1256.007 kb on - strand, within Pf6N2E2_1075 at 1256.016 kb on - strand, within Pf6N2E2_1075 at 1256.522 kb on - strand, within Pf6N2E2_1075 at 1256.681 kb on + strand, within Pf6N2E2_1075 at 1256.787 kb on - strand, within Pf6N2E2_1075 at 1256.921 kb on + strand, within Pf6N2E2_1075 at 1256.922 kb on - strand, within Pf6N2E2_1075 at 1257.068 kb on + strand, within Pf6N2E2_1075 at 1257.068 kb on + strand, within Pf6N2E2_1075 at 1257.169 kb on - strand at 1257.302 kb on + strand at 1257.302 kb on + strand at 1257.302 kb on + strand at 1257.302 kb on + strand at 1257.303 kb on - strand at 1257.465 kb on + strand at 1258.072 kb on + strand, within Pf6N2E2_1076 at 1258.072 kb on + strand, within Pf6N2E2_1076 at 1258.072 kb on + strand, within Pf6N2E2_1076 at 1258.073 kb on - strand, within Pf6N2E2_1076
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 2.5 mM remove 1,253,765 + Pf6N2E2_1073 0.87 +0.5 1,253,780 + Pf6N2E2_1073 0.88 -0.8 1,253,780 + Pf6N2E2_1073 0.88 +0.7 1,253,780 + Pf6N2E2_1073 0.88 -0.3 1,253,780 + Pf6N2E2_1073 0.88 +0.7 1,253,780 + Pf6N2E2_1073 0.88 -0.5 1,253,780 + Pf6N2E2_1073 0.88 +0.3 1,253,780 + Pf6N2E2_1073 0.88 -0.3 1,253,780 + Pf6N2E2_1073 0.88 +0.2 1,253,780 + Pf6N2E2_1073 0.88 -1.9 1,253,781 - Pf6N2E2_1073 0.88 -0.6 1,253,781 - Pf6N2E2_1073 0.88 +0.7 1,253,781 - Pf6N2E2_1073 0.88 +0.5 1,253,819 + Pf6N2E2_1073 0.90 +0.7 1,253,834 + +0.2 1,253,834 + -1.9 1,254,036 + -0.1 1,254,036 + +0.1 1,254,036 + +0.4 1,254,036 + +1.1 1,254,036 + -0.5 1,254,037 - -0.3 1,254,037 - +2.4 1,254,132 + -0.2 1,254,133 - +0.7 1,254,133 - +0.7 1,254,560 - Pf6N2E2_1074 0.83 +0.1 1,254,583 - Pf6N2E2_1074 0.90 -3.3 1,254,850 + -0.9 1,254,850 + +2.8 1,254,851 - -1.3 1,254,851 - +0.7 1,254,851 - +0.7 1,254,955 + Pf6N2E2_1075 0.11 +0.7 1,254,955 + Pf6N2E2_1075 0.11 +0.5 1,254,956 - Pf6N2E2_1075 0.11 -0.6 1,255,149 - Pf6N2E2_1075 0.18 -0.3 1,255,149 - Pf6N2E2_1075 0.18 -0.0 1,255,316 + Pf6N2E2_1075 0.24 +0.7 1,255,556 + Pf6N2E2_1075 0.33 +1.7 1,255,557 - Pf6N2E2_1075 0.33 +1.7 1,255,721 + Pf6N2E2_1075 0.39 -0.3 1,256,006 + Pf6N2E2_1075 0.49 +0.7 1,256,006 + Pf6N2E2_1075 0.49 +0.9 1,256,006 + Pf6N2E2_1075 0.49 +0.2 1,256,006 + Pf6N2E2_1075 0.49 +0.1 1,256,006 + Pf6N2E2_1075 0.49 -0.1 1,256,007 - Pf6N2E2_1075 0.49 -0.1 1,256,016 - Pf6N2E2_1075 0.49 +0.0 1,256,522 - Pf6N2E2_1075 0.68 -0.2 1,256,681 + Pf6N2E2_1075 0.73 -0.5 1,256,787 - Pf6N2E2_1075 0.77 -1.3 1,256,921 + Pf6N2E2_1075 0.82 +0.7 1,256,922 - Pf6N2E2_1075 0.82 +0.7 1,257,068 + Pf6N2E2_1075 0.87 +1.2 1,257,068 + Pf6N2E2_1075 0.87 +1.0 1,257,169 - +0.2 1,257,302 + -0.9 1,257,302 + -0.1 1,257,302 + -0.4 1,257,302 + +0.4 1,257,303 - +2.2 1,257,465 + -0.8 1,258,072 + Pf6N2E2_1076 0.31 -0.6 1,258,072 + Pf6N2E2_1076 0.31 +0.4 1,258,072 + Pf6N2E2_1076 0.31 -0.9 1,258,073 - Pf6N2E2_1076 0.31 +0.9
Or see this region's nucleotide sequence