Experiment: Carbon source Sucrose 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_874 and Pf6N2E2_875 are separated by 60 nucleotides Pf6N2E2_875 and Pf6N2E2_876 overlap by 4 nucleotides Pf6N2E2_876 and Pf6N2E2_877 are separated by 19 nucleotides Pf6N2E2_877 and Pf6N2E2_878 are separated by 76 nucleotides
Pf6N2E2_874 - Predicted cobalt transporter CbtC, at 1,024,468 to 1,025,145
_874
Pf6N2E2_875 - putative metal chaperone, involved in Fe-nitrile hydratase activation, GTPase of COG0523 family, at 1,025,206 to 1,025,646
_875
Pf6N2E2_876 - Cobalt-containing nitrile hydratase subunit beta (EC 4.2.1.84), at 1,025,643 to 1,026,296
_876
Pf6N2E2_877 - Cobalt-containing nitrile hydratase subunit alpha (EC 4.2.1.84), at 1,026,316 to 1,026,948
_877
Pf6N2E2_878 - Amidase clustered with urea ABC transporter and nitrile hydratase functions, at 1,027,025 to 1,028,539
_878
Position (kb)
1025
1026
1027 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1024.643 kb on + strand, within Pf6N2E2_874 at 1024.644 kb on - strand, within Pf6N2E2_874 at 1024.713 kb on + strand, within Pf6N2E2_874 at 1024.714 kb on - strand, within Pf6N2E2_874 at 1024.714 kb on - strand, within Pf6N2E2_874 at 1024.715 kb on + strand, within Pf6N2E2_874 at 1024.715 kb on + strand, within Pf6N2E2_874 at 1024.715 kb on + strand, within Pf6N2E2_874 at 1024.716 kb on - strand, within Pf6N2E2_874 at 1024.716 kb on - strand, within Pf6N2E2_874 at 1024.716 kb on - strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.731 kb on - strand, within Pf6N2E2_874 at 1024.731 kb on - strand, within Pf6N2E2_874 at 1024.814 kb on + strand, within Pf6N2E2_874 at 1024.815 kb on - strand, within Pf6N2E2_874 at 1024.963 kb on + strand, within Pf6N2E2_874 at 1024.984 kb on + strand, within Pf6N2E2_874 at 1024.984 kb on + strand, within Pf6N2E2_874 at 1024.985 kb on - strand, within Pf6N2E2_874 at 1024.985 kb on - strand, within Pf6N2E2_874 at 1025.069 kb on + strand, within Pf6N2E2_874 at 1025.096 kb on + strand at 1025.096 kb on + strand at 1025.096 kb on + strand at 1025.096 kb on + strand at 1025.096 kb on + strand at 1025.096 kb on + strand at 1025.097 kb on - strand at 1025.097 kb on - strand at 1025.130 kb on + strand at 1025.322 kb on + strand, within Pf6N2E2_875 at 1025.330 kb on + strand, within Pf6N2E2_875 at 1025.330 kb on + strand, within Pf6N2E2_875 at 1025.331 kb on - strand, within Pf6N2E2_875 at 1025.331 kb on - strand, within Pf6N2E2_875 at 1025.331 kb on - strand, within Pf6N2E2_875 at 1025.331 kb on - strand, within Pf6N2E2_875 at 1025.365 kb on - strand, within Pf6N2E2_875 at 1025.365 kb on - strand, within Pf6N2E2_875 at 1025.432 kb on + strand, within Pf6N2E2_875 at 1025.432 kb on + strand, within Pf6N2E2_875 at 1025.432 kb on + strand, within Pf6N2E2_875 at 1025.432 kb on + strand, within Pf6N2E2_875 at 1025.432 kb on + strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.433 kb on - strand, within Pf6N2E2_875 at 1025.491 kb on + strand, within Pf6N2E2_875 at 1025.492 kb on - strand, within Pf6N2E2_875 at 1025.621 kb on + strand at 1025.622 kb on - strand at 1025.622 kb on - strand at 1025.622 kb on - strand at 1025.622 kb on - strand at 1025.647 kb on - strand at 1025.660 kb on - strand at 1025.660 kb on - strand at 1025.660 kb on - strand at 1025.670 kb on + strand at 1025.670 kb on + strand at 1025.737 kb on + strand, within Pf6N2E2_876 at 1025.761 kb on - strand, within Pf6N2E2_876 at 1025.976 kb on + strand, within Pf6N2E2_876 at 1026.092 kb on - strand, within Pf6N2E2_876 at 1026.095 kb on - strand, within Pf6N2E2_876 at 1026.095 kb on - strand, within Pf6N2E2_876 at 1026.109 kb on + strand, within Pf6N2E2_876 at 1026.110 kb on - strand, within Pf6N2E2_876 at 1026.110 kb on - strand, within Pf6N2E2_876 at 1026.110 kb on - strand, within Pf6N2E2_876 at 1026.269 kb on + strand at 1026.269 kb on + strand at 1026.270 kb on - strand at 1026.277 kb on - strand at 1026.286 kb on - strand at 1026.434 kb on - strand, within Pf6N2E2_877 at 1026.472 kb on + strand, within Pf6N2E2_877 at 1026.472 kb on + strand, within Pf6N2E2_877 at 1026.472 kb on + strand, within Pf6N2E2_877 at 1026.472 kb on + strand, within Pf6N2E2_877 at 1026.473 kb on - strand, within Pf6N2E2_877 at 1026.493 kb on - strand, within Pf6N2E2_877 at 1026.493 kb on - strand, within Pf6N2E2_877 at 1026.563 kb on + strand, within Pf6N2E2_877 at 1026.563 kb on + strand, within Pf6N2E2_877 at 1026.737 kb on + strand, within Pf6N2E2_877 at 1026.738 kb on - strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.803 kb on + strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.804 kb on - strand, within Pf6N2E2_877 at 1026.940 kb on - strand at 1026.953 kb on + strand at 1026.953 kb on + strand at 1026.954 kb on - strand at 1026.971 kb on + strand at 1026.987 kb on + strand at 1026.987 kb on + strand at 1026.988 kb on - strand at 1026.988 kb on - strand at 1026.988 kb on - strand at 1027.026 kb on + strand at 1027.027 kb on - strand at 1027.092 kb on + strand at 1027.171 kb on + strand at 1027.296 kb on + strand, within Pf6N2E2_878 at 1027.296 kb on + strand, within Pf6N2E2_878 at 1027.296 kb on + strand, within Pf6N2E2_878
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 5 mM remove 1,024,643 + Pf6N2E2_874 0.26 +0.8 1,024,644 - Pf6N2E2_874 0.26 -0.2 1,024,713 + Pf6N2E2_874 0.36 +0.2 1,024,714 - Pf6N2E2_874 0.36 +0.1 1,024,714 - Pf6N2E2_874 0.36 +2.5 1,024,715 + Pf6N2E2_874 0.36 -1.3 1,024,715 + Pf6N2E2_874 0.36 +0.8 1,024,715 + Pf6N2E2_874 0.36 +0.8 1,024,716 - Pf6N2E2_874 0.37 -0.8 1,024,716 - Pf6N2E2_874 0.37 +0.4 1,024,716 - Pf6N2E2_874 0.37 +0.8 1,024,730 + Pf6N2E2_874 0.39 -0.3 1,024,730 + Pf6N2E2_874 0.39 -0.8 1,024,730 + Pf6N2E2_874 0.39 +0.8 1,024,730 + Pf6N2E2_874 0.39 -0.4 1,024,730 + Pf6N2E2_874 0.39 -0.8 1,024,730 + Pf6N2E2_874 0.39 -0.9 1,024,731 - Pf6N2E2_874 0.39 -0.4 1,024,731 - Pf6N2E2_874 0.39 +0.8 1,024,814 + Pf6N2E2_874 0.51 -0.8 1,024,815 - Pf6N2E2_874 0.51 -0.4 1,024,963 + Pf6N2E2_874 0.73 +0.8 1,024,984 + Pf6N2E2_874 0.76 -0.8 1,024,984 + Pf6N2E2_874 0.76 -0.6 1,024,985 - Pf6N2E2_874 0.76 -0.2 1,024,985 - Pf6N2E2_874 0.76 +0.3 1,025,069 + Pf6N2E2_874 0.89 -0.5 1,025,096 + -0.2 1,025,096 + -1.2 1,025,096 + -0.2 1,025,096 + -1.7 1,025,096 + +1.7 1,025,096 + -0.7 1,025,097 - +0.2 1,025,097 - +1.4 1,025,130 + +0.3 1,025,322 + Pf6N2E2_875 0.26 +0.8 1,025,330 + Pf6N2E2_875 0.28 +0.8 1,025,330 + Pf6N2E2_875 0.28 -0.5 1,025,331 - Pf6N2E2_875 0.28 -0.1 1,025,331 - Pf6N2E2_875 0.28 -0.2 1,025,331 - Pf6N2E2_875 0.28 -0.6 1,025,331 - Pf6N2E2_875 0.28 +0.1 1,025,365 - Pf6N2E2_875 0.36 +0.8 1,025,365 - Pf6N2E2_875 0.36 -0.1 1,025,432 + Pf6N2E2_875 0.51 +0.4 1,025,432 + Pf6N2E2_875 0.51 +0.4 1,025,432 + Pf6N2E2_875 0.51 +0.1 1,025,432 + Pf6N2E2_875 0.51 +0.1 1,025,432 + Pf6N2E2_875 0.51 +0.8 1,025,433 - Pf6N2E2_875 0.51 -1.2 1,025,433 - Pf6N2E2_875 0.51 -0.2 1,025,433 - Pf6N2E2_875 0.51 -1.0 1,025,433 - Pf6N2E2_875 0.51 -0.5 1,025,433 - Pf6N2E2_875 0.51 +0.8 1,025,433 - Pf6N2E2_875 0.51 +0.0 1,025,433 - Pf6N2E2_875 0.51 +0.2 1,025,433 - Pf6N2E2_875 0.51 +0.3 1,025,491 + Pf6N2E2_875 0.65 +0.9 1,025,492 - Pf6N2E2_875 0.65 -0.4 1,025,621 + -0.5 1,025,622 - -0.5 1,025,622 - -0.2 1,025,622 - +1.4 1,025,622 - -0.4 1,025,647 - +1.3 1,025,660 - +0.4 1,025,660 - +1.0 1,025,660 - -0.2 1,025,670 + +0.8 1,025,670 + -1.2 1,025,737 + Pf6N2E2_876 0.14 +0.5 1,025,761 - Pf6N2E2_876 0.18 +1.1 1,025,976 + Pf6N2E2_876 0.51 -2.4 1,026,092 - Pf6N2E2_876 0.69 +0.6 1,026,095 - Pf6N2E2_876 0.69 +1.4 1,026,095 - Pf6N2E2_876 0.69 +1.1 1,026,109 + Pf6N2E2_876 0.71 +0.8 1,026,110 - Pf6N2E2_876 0.71 -0.1 1,026,110 - Pf6N2E2_876 0.71 +0.6 1,026,110 - Pf6N2E2_876 0.71 -1.8 1,026,269 + -0.5 1,026,269 + +2.4 1,026,270 - +0.8 1,026,277 - +0.1 1,026,286 - +0.8 1,026,434 - Pf6N2E2_877 0.19 +0.0 1,026,472 + Pf6N2E2_877 0.25 +1.0 1,026,472 + Pf6N2E2_877 0.25 -1.4 1,026,472 + Pf6N2E2_877 0.25 +0.2 1,026,472 + Pf6N2E2_877 0.25 +0.8 1,026,473 - Pf6N2E2_877 0.25 +1.0 1,026,493 - Pf6N2E2_877 0.28 -0.8 1,026,493 - Pf6N2E2_877 0.28 -0.0 1,026,563 + Pf6N2E2_877 0.39 -0.3 1,026,563 + Pf6N2E2_877 0.39 +0.8 1,026,737 + Pf6N2E2_877 0.67 -0.9 1,026,738 - Pf6N2E2_877 0.67 +1.4 1,026,803 + Pf6N2E2_877 0.77 +0.0 1,026,803 + Pf6N2E2_877 0.77 -1.8 1,026,803 + Pf6N2E2_877 0.77 -0.2 1,026,803 + Pf6N2E2_877 0.77 -1.8 1,026,803 + Pf6N2E2_877 0.77 -0.5 1,026,803 + Pf6N2E2_877 0.77 -1.2 1,026,804 - Pf6N2E2_877 0.77 -0.2 1,026,804 - Pf6N2E2_877 0.77 +0.8 1,026,804 - Pf6N2E2_877 0.77 -2.5 1,026,804 - Pf6N2E2_877 0.77 -0.0 1,026,804 - Pf6N2E2_877 0.77 +0.2 1,026,804 - Pf6N2E2_877 0.77 -1.8 1,026,940 - +0.8 1,026,953 + -0.8 1,026,953 + +1.1 1,026,954 - -0.2 1,026,971 + +0.5 1,026,987 + -0.5 1,026,987 + +0.4 1,026,988 - -0.2 1,026,988 - +0.3 1,026,988 - +0.8 1,027,026 + +0.3 1,027,027 - -0.2 1,027,092 + +0.0 1,027,171 + +0.8 1,027,296 + Pf6N2E2_878 0.18 +0.8 1,027,296 + Pf6N2E2_878 0.18 +1.5 1,027,296 + Pf6N2E2_878 0.18 +0.8
Or see this region's nucleotide sequence