Experiment: Carbon source Sucrose 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5574 and Pf6N2E2_5575 are separated by 93 nucleotides Pf6N2E2_5575 and Pf6N2E2_5576 are separated by 323 nucleotides Pf6N2E2_5576 and Pf6N2E2_5577 are separated by 175 nucleotides
Pf6N2E2_5574 - Glycerol kinase (EC 2.7.1.30), at 6,392,556 to 6,394,061
_5574
Pf6N2E2_5575 - Glycerol uptake facilitator protein, at 6,394,155 to 6,395,006
_5575
Pf6N2E2_5576 - Cys-tRNA(Pro) deacylase YbaK, at 6,395,330 to 6,395,800
_5576
Pf6N2E2_5577 - Uncharacterized isomerase yddE, PhzC-PhzF family, at 6,395,976 to 6,396,758
_5577
Position (kb)
6394
6395
6396 Strain fitness (log2 ratio)
-2
-1
0
1 at 6393.274 kb on + strand, within Pf6N2E2_5574 at 6393.275 kb on - strand, within Pf6N2E2_5574 at 6393.379 kb on + strand, within Pf6N2E2_5574 at 6393.379 kb on + strand, within Pf6N2E2_5574 at 6393.379 kb on + strand, within Pf6N2E2_5574 at 6393.379 kb on + strand, within Pf6N2E2_5574 at 6393.379 kb on + strand, within Pf6N2E2_5574 at 6393.380 kb on - strand, within Pf6N2E2_5574 at 6393.502 kb on + strand, within Pf6N2E2_5574 at 6393.502 kb on + strand, within Pf6N2E2_5574 at 6393.503 kb on - strand, within Pf6N2E2_5574 at 6393.647 kb on - strand, within Pf6N2E2_5574 at 6393.647 kb on - strand, within Pf6N2E2_5574 at 6393.682 kb on + strand, within Pf6N2E2_5574 at 6393.682 kb on + strand, within Pf6N2E2_5574 at 6393.682 kb on + strand, within Pf6N2E2_5574 at 6393.682 kb on + strand, within Pf6N2E2_5574 at 6393.682 kb on + strand, within Pf6N2E2_5574 at 6393.682 kb on + strand, within Pf6N2E2_5574 at 6393.683 kb on - strand, within Pf6N2E2_5574 at 6393.683 kb on - strand, within Pf6N2E2_5574 at 6393.683 kb on - strand, within Pf6N2E2_5574 at 6393.683 kb on - strand, within Pf6N2E2_5574 at 6393.725 kb on + strand, within Pf6N2E2_5574 at 6393.758 kb on - strand, within Pf6N2E2_5574 at 6393.758 kb on - strand, within Pf6N2E2_5574 at 6394.036 kb on + strand at 6394.036 kb on + strand at 6394.036 kb on + strand at 6394.036 kb on + strand at 6394.036 kb on + strand at 6394.036 kb on + strand at 6394.036 kb on + strand at 6394.036 kb on + strand at 6394.036 kb on + strand at 6394.037 kb on - strand at 6394.037 kb on - strand at 6394.037 kb on - strand at 6394.037 kb on - strand at 6394.037 kb on - strand at 6394.037 kb on - strand at 6394.037 kb on - strand at 6394.050 kb on + strand at 6394.050 kb on + strand at 6394.050 kb on + strand at 6394.050 kb on + strand at 6394.050 kb on + strand at 6394.051 kb on - strand at 6394.051 kb on - strand at 6394.051 kb on - strand at 6394.051 kb on - strand at 6394.052 kb on + strand at 6394.083 kb on + strand at 6394.084 kb on - strand at 6394.084 kb on - strand at 6394.085 kb on + strand at 6394.085 kb on + strand at 6394.097 kb on + strand at 6394.097 kb on + strand at 6394.097 kb on + strand at 6394.097 kb on + strand at 6394.097 kb on + strand at 6394.097 kb on + strand at 6394.097 kb on + strand at 6394.097 kb on + strand at 6394.102 kb on - strand at 6394.102 kb on - strand at 6394.102 kb on - strand at 6394.102 kb on - strand at 6394.203 kb on - strand at 6394.209 kb on + strand at 6394.210 kb on - strand at 6394.210 kb on - strand at 6394.304 kb on - strand, within Pf6N2E2_5575 at 6394.665 kb on + strand, within Pf6N2E2_5575 at 6394.665 kb on + strand, within Pf6N2E2_5575 at 6394.666 kb on - strand, within Pf6N2E2_5575 at 6394.666 kb on - strand, within Pf6N2E2_5575 at 6394.666 kb on - strand, within Pf6N2E2_5575 at 6394.675 kb on + strand, within Pf6N2E2_5575 at 6394.676 kb on - strand, within Pf6N2E2_5575 at 6394.684 kb on + strand, within Pf6N2E2_5575 at 6394.684 kb on + strand, within Pf6N2E2_5575 at 6394.685 kb on - strand, within Pf6N2E2_5575 at 6394.730 kb on + strand, within Pf6N2E2_5575 at 6394.730 kb on + strand, within Pf6N2E2_5575 at 6394.731 kb on - strand, within Pf6N2E2_5575 at 6394.732 kb on + strand, within Pf6N2E2_5575 at 6394.732 kb on + strand, within Pf6N2E2_5575 at 6394.732 kb on + strand, within Pf6N2E2_5575 at 6394.732 kb on + strand, within Pf6N2E2_5575 at 6394.732 kb on + strand, within Pf6N2E2_5575 at 6394.733 kb on - strand, within Pf6N2E2_5575 at 6394.733 kb on - strand, within Pf6N2E2_5575 at 6394.733 kb on - strand, within Pf6N2E2_5575 at 6394.733 kb on - strand, within Pf6N2E2_5575 at 6394.733 kb on - strand, within Pf6N2E2_5575 at 6394.733 kb on - strand, within Pf6N2E2_5575 at 6394.733 kb on - strand, within Pf6N2E2_5575 at 6394.733 kb on - strand, within Pf6N2E2_5575 at 6394.829 kb on - strand, within Pf6N2E2_5575 at 6394.938 kb on + strand at 6394.992 kb on + strand at 6394.992 kb on + strand at 6395.045 kb on + strand at 6395.142 kb on + strand at 6395.145 kb on - strand at 6395.200 kb on + strand at 6395.200 kb on + strand at 6395.201 kb on - strand at 6395.201 kb on - strand at 6395.201 kb on - strand at 6395.285 kb on + strand at 6395.286 kb on - strand at 6395.353 kb on + strand at 6395.353 kb on + strand at 6395.353 kb on + strand at 6395.388 kb on - strand, within Pf6N2E2_5576 at 6395.414 kb on + strand, within Pf6N2E2_5576 at 6395.415 kb on - strand, within Pf6N2E2_5576 at 6395.619 kb on + strand, within Pf6N2E2_5576 at 6395.620 kb on - strand, within Pf6N2E2_5576 at 6395.620 kb on - strand, within Pf6N2E2_5576 at 6395.751 kb on + strand, within Pf6N2E2_5576 at 6395.858 kb on + strand at 6395.891 kb on + strand at 6395.891 kb on + strand at 6395.892 kb on - strand at 6395.906 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 5 mM remove 6,393,274 + Pf6N2E2_5574 0.48 +0.4 6,393,275 - Pf6N2E2_5574 0.48 +0.4 6,393,379 + Pf6N2E2_5574 0.55 -0.2 6,393,379 + Pf6N2E2_5574 0.55 -0.9 6,393,379 + Pf6N2E2_5574 0.55 +1.4 6,393,379 + Pf6N2E2_5574 0.55 +0.4 6,393,379 + Pf6N2E2_5574 0.55 -0.2 6,393,380 - Pf6N2E2_5574 0.55 +0.4 6,393,502 + Pf6N2E2_5574 0.63 +0.4 6,393,502 + Pf6N2E2_5574 0.63 +1.4 6,393,503 - Pf6N2E2_5574 0.63 -0.2 6,393,647 - Pf6N2E2_5574 0.72 +1.4 6,393,647 - Pf6N2E2_5574 0.72 -0.6 6,393,682 + Pf6N2E2_5574 0.75 +1.4 6,393,682 + Pf6N2E2_5574 0.75 +1.4 6,393,682 + Pf6N2E2_5574 0.75 -1.1 6,393,682 + Pf6N2E2_5574 0.75 -0.9 6,393,682 + Pf6N2E2_5574 0.75 +0.4 6,393,682 + Pf6N2E2_5574 0.75 +1.4 6,393,683 - Pf6N2E2_5574 0.75 +0.4 6,393,683 - Pf6N2E2_5574 0.75 -0.2 6,393,683 - Pf6N2E2_5574 0.75 +1.4 6,393,683 - Pf6N2E2_5574 0.75 +0.4 6,393,725 + Pf6N2E2_5574 0.78 +1.4 6,393,758 - Pf6N2E2_5574 0.80 +0.4 6,393,758 - Pf6N2E2_5574 0.80 -1.2 6,394,036 + +1.4 6,394,036 + +0.4 6,394,036 + -0.6 6,394,036 + +1.4 6,394,036 + -0.6 6,394,036 + -0.6 6,394,036 + +1.4 6,394,036 + -0.9 6,394,036 + -0.2 6,394,037 - +0.4 6,394,037 - -0.2 6,394,037 - +0.1 6,394,037 - -1.4 6,394,037 - -0.1 6,394,037 - +0.4 6,394,037 - -0.6 6,394,050 + -0.2 6,394,050 + +0.4 6,394,050 + -0.2 6,394,050 + -0.2 6,394,050 + +1.4 6,394,051 - +1.2 6,394,051 - +1.4 6,394,051 - -0.9 6,394,051 - +1.4 6,394,052 + -0.6 6,394,083 + +0.4 6,394,084 - +1.4 6,394,084 - -0.6 6,394,085 + -0.9 6,394,085 + +0.4 6,394,097 + +0.4 6,394,097 + -0.2 6,394,097 + +1.4 6,394,097 + -0.2 6,394,097 + +0.4 6,394,097 + -0.5 6,394,097 + +0.4 6,394,097 + +1.4 6,394,102 - +0.4 6,394,102 - +1.4 6,394,102 - +0.8 6,394,102 - +0.5 6,394,203 - +1.4 6,394,209 + +0.4 6,394,210 - +0.4 6,394,210 - -1.8 6,394,304 - Pf6N2E2_5575 0.17 +0.4 6,394,665 + Pf6N2E2_5575 0.60 +0.4 6,394,665 + Pf6N2E2_5575 0.60 +1.4 6,394,666 - Pf6N2E2_5575 0.60 +0.4 6,394,666 - Pf6N2E2_5575 0.60 -0.6 6,394,666 - Pf6N2E2_5575 0.60 -0.9 6,394,675 + Pf6N2E2_5575 0.61 -1.1 6,394,676 - Pf6N2E2_5575 0.61 +0.4 6,394,684 + Pf6N2E2_5575 0.62 +0.4 6,394,684 + Pf6N2E2_5575 0.62 +0.4 6,394,685 - Pf6N2E2_5575 0.62 +1.4 6,394,730 + Pf6N2E2_5575 0.67 -0.2 6,394,730 + Pf6N2E2_5575 0.67 +0.4 6,394,731 - Pf6N2E2_5575 0.68 -0.6 6,394,732 + Pf6N2E2_5575 0.68 +1.4 6,394,732 + Pf6N2E2_5575 0.68 +0.4 6,394,732 + Pf6N2E2_5575 0.68 +1.4 6,394,732 + Pf6N2E2_5575 0.68 -0.2 6,394,732 + Pf6N2E2_5575 0.68 +1.4 6,394,733 - Pf6N2E2_5575 0.68 +1.4 6,394,733 - Pf6N2E2_5575 0.68 -0.6 6,394,733 - Pf6N2E2_5575 0.68 +1.4 6,394,733 - Pf6N2E2_5575 0.68 +1.4 6,394,733 - Pf6N2E2_5575 0.68 +1.4 6,394,733 - Pf6N2E2_5575 0.68 +1.4 6,394,733 - Pf6N2E2_5575 0.68 -1.2 6,394,733 - Pf6N2E2_5575 0.68 +1.4 6,394,829 - Pf6N2E2_5575 0.79 +1.4 6,394,938 + +1.4 6,394,992 + +1.4 6,394,992 + +1.4 6,395,045 + +1.4 6,395,142 + +0.4 6,395,145 - +0.6 6,395,200 + +1.4 6,395,200 + +1.4 6,395,201 - +0.5 6,395,201 - +0.4 6,395,201 - -1.6 6,395,285 + +0.3 6,395,286 - +1.4 6,395,353 + -0.2 6,395,353 + +0.4 6,395,353 + -0.2 6,395,388 - Pf6N2E2_5576 0.12 -0.8 6,395,414 + Pf6N2E2_5576 0.18 +0.4 6,395,415 - Pf6N2E2_5576 0.18 +0.4 6,395,619 + Pf6N2E2_5576 0.61 +1.5 6,395,620 - Pf6N2E2_5576 0.62 +0.7 6,395,620 - Pf6N2E2_5576 0.62 +0.0 6,395,751 + Pf6N2E2_5576 0.89 +0.5 6,395,858 + -0.2 6,395,891 + -0.5 6,395,891 + -0.6 6,395,892 - +0.2 6,395,906 - +0.4
Or see this region's nucleotide sequence