Experiment: Carbon source Sucrose 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_351 and Pf6N2E2_352 are separated by 203 nucleotides Pf6N2E2_352 and Pf6N2E2_353 are separated by 166 nucleotides Pf6N2E2_353 and Pf6N2E2_354 overlap by 11 nucleotides
Pf6N2E2_351 - Aldehyde dehydrogenase (EC 1.2.1.3), at 416,238 to 417,686
_351
Pf6N2E2_352 - Opine oxidase subunit B, at 417,890 to 419,071
_352
Pf6N2E2_353 - Bona fide RidA/YjgF/TdcF/RutC subgroup, at 419,238 to 419,642
_353
Pf6N2E2_354 - Type III PLP / low-specificity D-threonine aldolase, at 419,632 to 420,711
_354
Position (kb) 417
418
419
420 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 417.212 kb on - strand, within Pf6N2E2_351 at 417.301 kb on + strand, within Pf6N2E2_351 at 417.301 kb on + strand, within Pf6N2E2_351 at 417.301 kb on + strand, within Pf6N2E2_351 at 417.302 kb on - strand, within Pf6N2E2_351 at 417.302 kb on - strand, within Pf6N2E2_351 at 417.330 kb on + strand, within Pf6N2E2_351 at 417.531 kb on + strand, within Pf6N2E2_351 at 417.640 kb on + strand at 417.640 kb on + strand at 417.640 kb on + strand at 417.640 kb on + strand at 417.641 kb on - strand at 417.641 kb on - strand at 417.641 kb on - strand at 417.641 kb on - strand at 417.641 kb on - strand at 417.701 kb on + strand at 417.701 kb on + strand at 417.712 kb on + strand at 417.712 kb on + strand at 417.713 kb on - strand at 417.713 kb on - strand at 417.759 kb on + strand at 417.760 kb on - strand at 417.760 kb on - strand at 417.781 kb on + strand at 417.818 kb on + strand at 417.819 kb on - strand at 417.819 kb on - strand at 418.075 kb on - strand, within Pf6N2E2_352 at 418.075 kb on - strand, within Pf6N2E2_352 at 418.131 kb on + strand, within Pf6N2E2_352 at 418.131 kb on + strand, within Pf6N2E2_352 at 418.132 kb on - strand, within Pf6N2E2_352 at 418.132 kb on - strand, within Pf6N2E2_352 at 418.132 kb on - strand, within Pf6N2E2_352 at 418.317 kb on + strand, within Pf6N2E2_352 at 418.318 kb on - strand, within Pf6N2E2_352 at 418.318 kb on - strand, within Pf6N2E2_352 at 418.335 kb on - strand, within Pf6N2E2_352 at 418.496 kb on + strand, within Pf6N2E2_352 at 418.497 kb on - strand, within Pf6N2E2_352 at 418.653 kb on + strand, within Pf6N2E2_352 at 418.654 kb on - strand, within Pf6N2E2_352 at 418.870 kb on - strand, within Pf6N2E2_352 at 418.870 kb on - strand, within Pf6N2E2_352 at 418.948 kb on - strand, within Pf6N2E2_352 at 419.048 kb on + strand at 419.048 kb on + strand at 419.048 kb on + strand at 419.048 kb on + strand at 419.049 kb on - strand at 419.049 kb on - strand at 419.049 kb on - strand at 419.075 kb on - strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.112 kb on + strand at 419.113 kb on - strand at 419.113 kb on - strand at 419.113 kb on - strand at 419.113 kb on - strand at 419.113 kb on - strand at 419.113 kb on - strand at 419.239 kb on + strand at 419.239 kb on + strand at 419.240 kb on - strand at 419.291 kb on - strand, within Pf6N2E2_353 at 419.291 kb on - strand, within Pf6N2E2_353 at 419.351 kb on - strand, within Pf6N2E2_353 at 419.359 kb on + strand, within Pf6N2E2_353 at 419.401 kb on + strand, within Pf6N2E2_353 at 419.401 kb on + strand, within Pf6N2E2_353 at 419.401 kb on + strand, within Pf6N2E2_353 at 419.401 kb on + strand, within Pf6N2E2_353 at 419.401 kb on + strand, within Pf6N2E2_353 at 419.402 kb on - strand, within Pf6N2E2_353 at 419.402 kb on - strand, within Pf6N2E2_353 at 419.402 kb on - strand, within Pf6N2E2_353 at 419.402 kb on - strand, within Pf6N2E2_353 at 419.551 kb on + strand, within Pf6N2E2_353 at 419.551 kb on + strand, within Pf6N2E2_353 at 419.552 kb on - strand, within Pf6N2E2_353 at 419.552 kb on - strand, within Pf6N2E2_353 at 419.552 kb on - strand, within Pf6N2E2_353 at 419.552 kb on - strand, within Pf6N2E2_353 at 419.584 kb on + strand, within Pf6N2E2_353 at 419.585 kb on - strand, within Pf6N2E2_353 at 419.691 kb on - strand at 419.691 kb on - strand at 419.691 kb on - strand at 420.059 kb on + strand, within Pf6N2E2_354 at 420.059 kb on + strand, within Pf6N2E2_354 at 420.059 kb on + strand, within Pf6N2E2_354 at 420.059 kb on + strand, within Pf6N2E2_354 at 420.059 kb on + strand, within Pf6N2E2_354 at 420.060 kb on - strand, within Pf6N2E2_354 at 420.060 kb on - strand, within Pf6N2E2_354 at 420.060 kb on - strand, within Pf6N2E2_354
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 5 mM remove 417,212 - Pf6N2E2_351 0.67 -1.0 417,301 + Pf6N2E2_351 0.73 -0.1 417,301 + Pf6N2E2_351 0.73 -0.0 417,301 + Pf6N2E2_351 0.73 +2.6 417,302 - Pf6N2E2_351 0.73 -0.8 417,302 - Pf6N2E2_351 0.73 -0.6 417,330 + Pf6N2E2_351 0.75 +0.7 417,531 + Pf6N2E2_351 0.89 +0.5 417,640 + -2.2 417,640 + -0.6 417,640 + +0.3 417,640 + +0.3 417,641 - +1.0 417,641 - -1.6 417,641 - +0.7 417,641 - +0.2 417,641 - +0.2 417,701 + +0.3 417,701 + +0.2 417,712 + +0.4 417,712 + -0.5 417,713 - +0.7 417,713 - -0.0 417,759 + +0.6 417,760 - +1.0 417,760 - +2.3 417,781 + +1.0 417,818 + -1.0 417,819 - +0.3 417,819 - +0.2 418,075 - Pf6N2E2_352 0.16 +1.0 418,075 - Pf6N2E2_352 0.16 -0.5 418,131 + Pf6N2E2_352 0.20 -0.0 418,131 + Pf6N2E2_352 0.20 +0.3 418,132 - Pf6N2E2_352 0.20 +0.6 418,132 - Pf6N2E2_352 0.20 +1.0 418,132 - Pf6N2E2_352 0.20 +0.4 418,317 + Pf6N2E2_352 0.36 -1.0 418,318 - Pf6N2E2_352 0.36 +3.0 418,318 - Pf6N2E2_352 0.36 -0.0 418,335 - Pf6N2E2_352 0.38 -0.0 418,496 + Pf6N2E2_352 0.51 +1.7 418,497 - Pf6N2E2_352 0.51 +1.0 418,653 + Pf6N2E2_352 0.65 +1.3 418,654 - Pf6N2E2_352 0.65 +0.8 418,870 - Pf6N2E2_352 0.83 +1.1 418,870 - Pf6N2E2_352 0.83 +1.2 418,948 - Pf6N2E2_352 0.90 -0.6 419,048 + -0.6 419,048 + -2.7 419,048 + -0.2 419,048 + +0.8 419,049 - -0.0 419,049 - -2.5 419,049 - -0.6 419,075 - -0.4 419,112 + -0.4 419,112 + +0.5 419,112 + +0.3 419,112 + +0.3 419,112 + +0.1 419,112 + -2.3 419,112 + +0.7 419,112 + +0.2 419,112 + -1.1 419,113 - -1.3 419,113 - +0.4 419,113 - +1.0 419,113 - +0.7 419,113 - -0.6 419,113 - +1.3 419,239 + +0.5 419,239 + -0.2 419,240 - +1.0 419,291 - Pf6N2E2_353 0.13 -1.1 419,291 - Pf6N2E2_353 0.13 +0.5 419,351 - Pf6N2E2_353 0.28 +0.7 419,359 + Pf6N2E2_353 0.30 -0.2 419,401 + Pf6N2E2_353 0.40 -0.8 419,401 + Pf6N2E2_353 0.40 -1.2 419,401 + Pf6N2E2_353 0.40 +1.0 419,401 + Pf6N2E2_353 0.40 +1.7 419,401 + Pf6N2E2_353 0.40 -0.2 419,402 - Pf6N2E2_353 0.40 -0.6 419,402 - Pf6N2E2_353 0.40 +0.3 419,402 - Pf6N2E2_353 0.40 -1.3 419,402 - Pf6N2E2_353 0.40 -0.1 419,551 + Pf6N2E2_353 0.77 +0.4 419,551 + Pf6N2E2_353 0.77 -1.4 419,552 - Pf6N2E2_353 0.78 +0.3 419,552 - Pf6N2E2_353 0.78 -0.5 419,552 - Pf6N2E2_353 0.78 -0.0 419,552 - Pf6N2E2_353 0.78 +0.1 419,584 + Pf6N2E2_353 0.85 +1.0 419,585 - Pf6N2E2_353 0.86 +0.6 419,691 - -0.0 419,691 - -0.0 419,691 - +1.4 420,059 + Pf6N2E2_354 0.40 -0.2 420,059 + Pf6N2E2_354 0.40 +0.1 420,059 + Pf6N2E2_354 0.40 -0.9 420,059 + Pf6N2E2_354 0.40 -0.0 420,059 + Pf6N2E2_354 0.40 -0.0 420,060 - Pf6N2E2_354 0.40 -0.3 420,060 - Pf6N2E2_354 0.40 -0.1 420,060 - Pf6N2E2_354 0.40 +0.6
Or see this region's nucleotide sequence