Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_322

Experiment: Carbon source Sucrose 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_321 and Pf6N2E2_322 are separated by 234 nucleotidesPf6N2E2_322 and Pf6N2E2_323 are separated by 166 nucleotides Pf6N2E2_321 - TRAP transporter solute receptor, unknown substrate 1, at 389,237 to 390,187 _321 Pf6N2E2_322 - Transcriptional regulator, LacI family, at 390,422 to 391,420 _322 Pf6N2E2_323 - Nucleoside-diphosphate-sugar epimerases, at 391,587 to 392,762 _323 Position (kb) 390 391 392Strain fitness (log2 ratio) -2 -1 0 1 2at 389.475 kb on + strand, within Pf6N2E2_321at 389.475 kb on + strand, within Pf6N2E2_321at 389.475 kb on + strand, within Pf6N2E2_321at 389.475 kb on + strand, within Pf6N2E2_321at 389.476 kb on - strand, within Pf6N2E2_321at 389.476 kb on - strand, within Pf6N2E2_321at 389.476 kb on - strand, within Pf6N2E2_321at 389.479 kb on + strand, within Pf6N2E2_321at 389.480 kb on - strand, within Pf6N2E2_321at 389.549 kb on + strand, within Pf6N2E2_321at 389.549 kb on + strand, within Pf6N2E2_321at 389.550 kb on - strand, within Pf6N2E2_321at 389.550 kb on - strand, within Pf6N2E2_321at 389.550 kb on - strand, within Pf6N2E2_321at 389.550 kb on - strand, within Pf6N2E2_321at 389.550 kb on - strand, within Pf6N2E2_321at 389.550 kb on - strand, within Pf6N2E2_321at 389.553 kb on + strand, within Pf6N2E2_321at 389.553 kb on + strand, within Pf6N2E2_321at 389.553 kb on + strand, within Pf6N2E2_321at 389.553 kb on + strand, within Pf6N2E2_321at 389.553 kb on + strand, within Pf6N2E2_321at 389.554 kb on - strand, within Pf6N2E2_321at 389.554 kb on - strand, within Pf6N2E2_321at 389.554 kb on - strand, within Pf6N2E2_321at 389.673 kb on + strand, within Pf6N2E2_321at 389.673 kb on + strand, within Pf6N2E2_321at 389.694 kb on + strand, within Pf6N2E2_321at 389.695 kb on - strand, within Pf6N2E2_321at 389.832 kb on + strand, within Pf6N2E2_321at 389.832 kb on + strand, within Pf6N2E2_321at 390.036 kb on + strand, within Pf6N2E2_321at 390.036 kb on + strand, within Pf6N2E2_321at 390.037 kb on - strand, within Pf6N2E2_321at 390.037 kb on - strand, within Pf6N2E2_321at 390.105 kb on + strandat 390.105 kb on + strandat 390.106 kb on - strandat 390.106 kb on - strandat 390.208 kb on - strandat 390.214 kb on + strandat 390.214 kb on + strandat 390.215 kb on - strandat 390.215 kb on - strandat 390.215 kb on - strandat 390.234 kb on + strandat 390.234 kb on + strandat 390.235 kb on - strandat 390.235 kb on - strandat 390.235 kb on - strandat 390.235 kb on - strandat 390.235 kb on - strandat 390.423 kb on + strandat 390.423 kb on + strandat 390.423 kb on + strandat 390.440 kb on - strandat 390.626 kb on - strand, within Pf6N2E2_322at 391.086 kb on + strand, within Pf6N2E2_322at 391.086 kb on + strand, within Pf6N2E2_322at 391.207 kb on + strand, within Pf6N2E2_322at 391.207 kb on + strand, within Pf6N2E2_322at 391.207 kb on + strand, within Pf6N2E2_322at 391.208 kb on - strand, within Pf6N2E2_322at 391.482 kb on + strandat 391.609 kb on + strandat 391.610 kb on - strandat 391.610 kb on - strandat 391.776 kb on + strand, within Pf6N2E2_323at 391.993 kb on - strand, within Pf6N2E2_323at 392.006 kb on + strand, within Pf6N2E2_323at 392.010 kb on + strand, within Pf6N2E2_323at 392.010 kb on + strand, within Pf6N2E2_323at 392.010 kb on + strand, within Pf6N2E2_323at 392.010 kb on + strand, within Pf6N2E2_323at 392.011 kb on - strand, within Pf6N2E2_323at 392.138 kb on + strand, within Pf6N2E2_323at 392.246 kb on + strand, within Pf6N2E2_323at 392.257 kb on + strand, within Pf6N2E2_323at 392.257 kb on + strand, within Pf6N2E2_323at 392.257 kb on + strand, within Pf6N2E2_323at 392.258 kb on - strand, within Pf6N2E2_323at 392.258 kb on - strand, within Pf6N2E2_323at 392.258 kb on - strand, within Pf6N2E2_323at 392.258 kb on - strand, within Pf6N2E2_323at 392.404 kb on - strand, within Pf6N2E2_323

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbon source Sucrose 5 mM
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389,475 + Pf6N2E2_321 0.25 +0.4
389,475 + Pf6N2E2_321 0.25 -1.6
389,475 + Pf6N2E2_321 0.25 -0.0
389,475 + Pf6N2E2_321 0.25 +0.0
389,476 - Pf6N2E2_321 0.25 -0.2
389,476 - Pf6N2E2_321 0.25 +0.0
389,476 - Pf6N2E2_321 0.25 -0.2
389,479 + Pf6N2E2_321 0.25 +1.0
389,480 - Pf6N2E2_321 0.26 +0.1
389,549 + Pf6N2E2_321 0.33 -0.5
389,549 + Pf6N2E2_321 0.33 +0.4
389,550 - Pf6N2E2_321 0.33 +1.0
389,550 - Pf6N2E2_321 0.33 +0.9
389,550 - Pf6N2E2_321 0.33 -0.6
389,550 - Pf6N2E2_321 0.33 +0.4
389,550 - Pf6N2E2_321 0.33 -0.8
389,550 - Pf6N2E2_321 0.33 +0.1
389,553 + Pf6N2E2_321 0.33 +2.0
389,553 + Pf6N2E2_321 0.33 +0.1
389,553 + Pf6N2E2_321 0.33 +0.0
389,553 + Pf6N2E2_321 0.33 -0.4
389,553 + Pf6N2E2_321 0.33 +1.0
389,554 - Pf6N2E2_321 0.33 +0.0
389,554 - Pf6N2E2_321 0.33 +0.6
389,554 - Pf6N2E2_321 0.33 -0.2
389,673 + Pf6N2E2_321 0.46 -0.6
389,673 + Pf6N2E2_321 0.46 +1.0
389,694 + Pf6N2E2_321 0.48 +0.6
389,695 - Pf6N2E2_321 0.48 -0.2
389,832 + Pf6N2E2_321 0.63 -1.4
389,832 + Pf6N2E2_321 0.63 -1.1
390,036 + Pf6N2E2_321 0.84 -1.0
390,036 + Pf6N2E2_321 0.84 +1.0
390,037 - Pf6N2E2_321 0.84 -2.3
390,037 - Pf6N2E2_321 0.84 +0.6
390,105 + +0.7
390,105 + +0.8
390,106 - +0.5
390,106 - -0.8
390,208 - +0.4
390,214 + -1.3
390,214 + +0.6
390,215 - -0.6
390,215 - -0.4
390,215 - -2.0
390,234 + +0.0
390,234 + -0.6
390,235 - +1.0
390,235 - +0.0
390,235 - +1.0
390,235 - +0.0
390,235 - -0.4
390,423 + -1.4
390,423 + -0.3
390,423 + +0.1
390,440 - -2.2
390,626 - Pf6N2E2_322 0.20 -0.6
391,086 + Pf6N2E2_322 0.66 +2.0
391,086 + Pf6N2E2_322 0.66 +1.2
391,207 + Pf6N2E2_322 0.79 -0.1
391,207 + Pf6N2E2_322 0.79 +1.0
391,207 + Pf6N2E2_322 0.79 -0.3
391,208 - Pf6N2E2_322 0.79 +0.5
391,482 + +1.0
391,609 + -1.0
391,610 - -0.6
391,610 - -0.7
391,776 + Pf6N2E2_323 0.16 +0.0
391,993 - Pf6N2E2_323 0.35 -1.0
392,006 + Pf6N2E2_323 0.36 -0.2
392,010 + Pf6N2E2_323 0.36 -0.3
392,010 + Pf6N2E2_323 0.36 +0.0
392,010 + Pf6N2E2_323 0.36 +1.5
392,010 + Pf6N2E2_323 0.36 -2.2
392,011 - Pf6N2E2_323 0.36 -0.7
392,138 + Pf6N2E2_323 0.47 -0.1
392,246 + Pf6N2E2_323 0.56 +1.4
392,257 + Pf6N2E2_323 0.57 +0.7
392,257 + Pf6N2E2_323 0.57 -1.0
392,257 + Pf6N2E2_323 0.57 +1.4
392,258 - Pf6N2E2_323 0.57 +0.5
392,258 - Pf6N2E2_323 0.57 -0.6
392,258 - Pf6N2E2_323 0.57 +1.0
392,258 - Pf6N2E2_323 0.57 -0.4
392,404 - Pf6N2E2_323 0.69 +0.0

Or see this region's nucleotide sequence