Experiment: Carbon source Sucrose 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_202 and Pf6N2E2_203 overlap by 11 nucleotides Pf6N2E2_203 and Pf6N2E2_204 are separated by 2 nucleotides Pf6N2E2_204 and Pf6N2E2_205 overlap by 4 nucleotides
Pf6N2E2_202 - 4Fe-4S ferredoxin, iron-sulfur binding, at 253,064 to 254,650
_202
Pf6N2E2_203 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 254,640 to 255,920
_203
Pf6N2E2_204 - hypothetical protein, at 255,923 to 256,330
_204
Pf6N2E2_205 - hypothetical protein, at 256,327 to 257,073
_205
Position (kb) 254
255
256 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 253.941 kb on + strand, within Pf6N2E2_202 at 253.941 kb on + strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 254.157 kb on + strand, within Pf6N2E2_202 at 254.157 kb on + strand, within Pf6N2E2_202 at 254.157 kb on + strand, within Pf6N2E2_202 at 254.157 kb on + strand, within Pf6N2E2_202 at 254.158 kb on - strand, within Pf6N2E2_202 at 254.158 kb on - strand, within Pf6N2E2_202 at 254.158 kb on - strand, within Pf6N2E2_202 at 254.218 kb on - strand, within Pf6N2E2_202 at 254.218 kb on - strand, within Pf6N2E2_202 at 254.239 kb on - strand, within Pf6N2E2_202 at 254.826 kb on + strand, within Pf6N2E2_203 at 254.960 kb on + strand, within Pf6N2E2_203 at 255.029 kb on - strand, within Pf6N2E2_203 at 255.232 kb on - strand, within Pf6N2E2_203 at 255.384 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.417 kb on - strand, within Pf6N2E2_203 at 255.514 kb on + strand, within Pf6N2E2_203 at 255.514 kb on + strand, within Pf6N2E2_203 at 255.514 kb on + strand, within Pf6N2E2_203 at 255.515 kb on - strand, within Pf6N2E2_203 at 255.515 kb on - strand, within Pf6N2E2_203 at 255.515 kb on - strand, within Pf6N2E2_203 at 255.803 kb on + strand at 255.803 kb on + strand at 255.803 kb on + strand at 255.803 kb on + strand at 255.803 kb on + strand at 255.803 kb on + strand at 255.803 kb on + strand at 255.805 kb on + strand at 255.805 kb on + strand at 255.805 kb on + strand at 255.805 kb on + strand at 255.806 kb on - strand at 255.806 kb on - strand at 255.806 kb on - strand at 255.890 kb on - strand at 255.912 kb on + strand at 256.062 kb on + strand, within Pf6N2E2_204 at 256.062 kb on + strand, within Pf6N2E2_204 at 256.062 kb on + strand, within Pf6N2E2_204 at 256.321 kb on + strand at 256.478 kb on + strand, within Pf6N2E2_205 at 256.478 kb on + strand, within Pf6N2E2_205 at 256.479 kb on - strand, within Pf6N2E2_205 at 256.562 kb on + strand, within Pf6N2E2_205 at 256.562 kb on + strand, within Pf6N2E2_205 at 256.563 kb on - strand, within Pf6N2E2_205 at 256.563 kb on - strand, within Pf6N2E2_205 at 256.563 kb on - strand, within Pf6N2E2_205 at 256.659 kb on + strand, within Pf6N2E2_205 at 256.661 kb on + strand, within Pf6N2E2_205 at 256.661 kb on + strand, within Pf6N2E2_205 at 256.882 kb on + strand, within Pf6N2E2_205 at 256.883 kb on - strand, within Pf6N2E2_205 at 256.892 kb on + strand, within Pf6N2E2_205
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 5 mM remove 253,941 + Pf6N2E2_202 0.55 +1.0 253,941 + Pf6N2E2_202 0.55 +0.5 253,942 - Pf6N2E2_202 0.55 +0.6 253,942 - Pf6N2E2_202 0.55 +0.0 253,942 - Pf6N2E2_202 0.55 -1.0 253,942 - Pf6N2E2_202 0.55 +1.0 253,942 - Pf6N2E2_202 0.55 +0.6 253,942 - Pf6N2E2_202 0.55 -0.0 253,942 - Pf6N2E2_202 0.55 -1.3 253,942 - Pf6N2E2_202 0.55 +1.0 253,942 - Pf6N2E2_202 0.55 +0.0 253,942 - Pf6N2E2_202 0.55 -0.5 254,157 + Pf6N2E2_202 0.69 -0.5 254,157 + Pf6N2E2_202 0.69 -0.3 254,157 + Pf6N2E2_202 0.69 -0.3 254,157 + Pf6N2E2_202 0.69 -1.0 254,158 - Pf6N2E2_202 0.69 +0.0 254,158 - Pf6N2E2_202 0.69 +1.7 254,158 - Pf6N2E2_202 0.69 +0.2 254,218 - Pf6N2E2_202 0.73 +0.5 254,218 - Pf6N2E2_202 0.73 +1.3 254,239 - Pf6N2E2_202 0.74 +0.2 254,826 + Pf6N2E2_203 0.15 -1.3 254,960 + Pf6N2E2_203 0.25 -0.5 255,029 - Pf6N2E2_203 0.30 -0.5 255,232 - Pf6N2E2_203 0.46 -0.7 255,384 + Pf6N2E2_203 0.58 -0.1 255,416 + Pf6N2E2_203 0.61 -0.7 255,416 + Pf6N2E2_203 0.61 -0.2 255,416 + Pf6N2E2_203 0.61 +0.3 255,416 + Pf6N2E2_203 0.61 -1.2 255,416 + Pf6N2E2_203 0.61 +0.2 255,416 + Pf6N2E2_203 0.61 +0.6 255,417 - Pf6N2E2_203 0.61 +1.0 255,514 + Pf6N2E2_203 0.68 -0.5 255,514 + Pf6N2E2_203 0.68 -1.0 255,514 + Pf6N2E2_203 0.68 -1.1 255,515 - Pf6N2E2_203 0.68 -0.4 255,515 - Pf6N2E2_203 0.68 +1.0 255,515 - Pf6N2E2_203 0.68 -1.1 255,803 + -1.0 255,803 + +0.0 255,803 + -0.3 255,803 + +0.1 255,803 + -0.2 255,803 + -0.7 255,803 + -0.0 255,805 + +0.5 255,805 + -0.6 255,805 + -0.7 255,805 + +0.0 255,806 - +0.5 255,806 - +1.0 255,806 - +0.6 255,890 - +0.7 255,912 + -0.3 256,062 + Pf6N2E2_204 0.34 +2.0 256,062 + Pf6N2E2_204 0.34 +0.0 256,062 + Pf6N2E2_204 0.34 +0.6 256,321 + +3.4 256,478 + Pf6N2E2_205 0.20 -1.8 256,478 + Pf6N2E2_205 0.20 +0.0 256,479 - Pf6N2E2_205 0.20 -0.2 256,562 + Pf6N2E2_205 0.31 +0.6 256,562 + Pf6N2E2_205 0.31 -1.4 256,563 - Pf6N2E2_205 0.32 -0.8 256,563 - Pf6N2E2_205 0.32 -0.4 256,563 - Pf6N2E2_205 0.32 +0.4 256,659 + Pf6N2E2_205 0.44 +0.3 256,661 + Pf6N2E2_205 0.45 -1.7 256,661 + Pf6N2E2_205 0.45 -0.4 256,882 + Pf6N2E2_205 0.74 -1.3 256,883 - Pf6N2E2_205 0.74 +1.0 256,892 + Pf6N2E2_205 0.76 -1.2
Or see this region's nucleotide sequence