Experiment: Vogels_fungal_media 0.1X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11950 and BWI76_RS11955 are separated by 135 nucleotides BWI76_RS11955 and BWI76_RS11960 are separated by 6 nucleotides BWI76_RS11960 and BWI76_RS11965 are separated by 191 nucleotides BWI76_RS11965 and BWI76_RS11970 overlap by 1 nucleotides
BWI76_RS11950: BWI76_RS11950 - phosphatidylglycerophosphatase B, at 2,516,820 to 2,517,587
_RS11950
BWI76_RS11955: BWI76_RS11955 - hypothetical protein, at 2,517,723 to 2,518,031
_RS11955
BWI76_RS11960: BWI76_RS11960 - lipopolysaccharide assembly protein LapB, at 2,518,038 to 2,519,207
_RS11960
BWI76_RS11965: BWI76_RS11965 - orotidine-5'-phosphate decarboxylase, at 2,519,399 to 2,520,136
_RS11965
BWI76_RS11970: BWI76_RS11970 - stress response translation initiation inhibitor YciH, at 2,520,136 to 2,520,462
_RS11970
Position (kb)
2518
2519
2520 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2517.084 kb on + strand, within BWI76_RS11950 at 2517.098 kb on - strand, within BWI76_RS11950 at 2517.339 kb on + strand, within BWI76_RS11950 at 2517.340 kb on + strand, within BWI76_RS11950 at 2517.345 kb on + strand, within BWI76_RS11950 at 2517.523 kb on - strand at 2517.610 kb on - strand at 2517.649 kb on - strand at 2519.432 kb on + strand at 2519.456 kb on + strand at 2519.791 kb on - strand, within BWI76_RS11965 at 2519.933 kb on + strand, within BWI76_RS11965 at 2519.960 kb on - strand, within BWI76_RS11965 at 2520.053 kb on + strand, within BWI76_RS11965
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.1X remove 2,517,084 + BWI76_RS11950 0.34 +0.3 2,517,098 - BWI76_RS11950 0.36 -0.2 2,517,339 + BWI76_RS11950 0.68 +0.1 2,517,340 + BWI76_RS11950 0.68 -1.8 2,517,345 + BWI76_RS11950 0.68 +0.4 2,517,523 - +0.6 2,517,610 - +0.5 2,517,649 - -0.2 2,519,432 + -2.8 2,519,456 + -3.6 2,519,791 - BWI76_RS11965 0.53 -4.7 2,519,933 + BWI76_RS11965 0.72 -4.6 2,519,960 - BWI76_RS11965 0.76 -5.1 2,520,053 + BWI76_RS11965 0.89 -3.5
Or see this region's nucleotide sequence