Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_386

Experiment: Carbon source D,L-Lactate 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_384 and Pf6N2E2_385 are separated by 97 nucleotidesPf6N2E2_385 and Pf6N2E2_386 are separated by 460 nucleotidesPf6N2E2_386 and Pf6N2E2_387 are separated by 3 nucleotides Pf6N2E2_384 - Glycine cleavage system transcriptional activator, at 451,638 to 451,979 _384 Pf6N2E2_385 - hypothetical protein, at 452,077 to 452,247 _385 Pf6N2E2_386 - similar to ribulose-1,5-bisphosphate carboxylase, Type III, at 452,708 to 453,985 _386 Pf6N2E2_387 - Candidate type III effector Hop protein, at 453,989 to 455,380 _387 Position (kb) 452 453 454Strain fitness (log2 ratio) -2 -1 0 1 2 3at 451.852 kb on + strandat 451.853 kb on - strand, within Pf6N2E2_384at 451.853 kb on - strand, within Pf6N2E2_384at 451.913 kb on - strand, within Pf6N2E2_384at 452.005 kb on + strandat 452.005 kb on + strandat 452.006 kb on - strandat 452.019 kb on + strandat 452.019 kb on + strandat 452.020 kb on - strandat 452.020 kb on - strandat 452.021 kb on + strandat 452.021 kb on + strandat 452.022 kb on - strandat 452.022 kb on - strandat 452.264 kb on + strandat 452.405 kb on + strandat 452.635 kb on - strandat 452.635 kb on - strandat 452.637 kb on - strandat 452.678 kb on - strandat 452.678 kb on - strandat 452.679 kb on + strandat 452.758 kb on - strandat 452.819 kb on - strandat 452.819 kb on - strandat 452.919 kb on + strand, within Pf6N2E2_386at 452.924 kb on - strand, within Pf6N2E2_386at 452.931 kb on - strand, within Pf6N2E2_386at 452.931 kb on - strand, within Pf6N2E2_386at 452.931 kb on - strand, within Pf6N2E2_386at 452.931 kb on - strand, within Pf6N2E2_386at 452.960 kb on + strand, within Pf6N2E2_386at 452.960 kb on + strand, within Pf6N2E2_386at 452.960 kb on + strand, within Pf6N2E2_386at 452.960 kb on + strand, within Pf6N2E2_386at 452.961 kb on - strand, within Pf6N2E2_386at 452.961 kb on - strand, within Pf6N2E2_386at 452.964 kb on - strand, within Pf6N2E2_386at 453.048 kb on + strand, within Pf6N2E2_386at 453.049 kb on - strand, within Pf6N2E2_386at 453.058 kb on - strand, within Pf6N2E2_386at 453.058 kb on - strand, within Pf6N2E2_386at 453.075 kb on + strand, within Pf6N2E2_386at 453.200 kb on - strand, within Pf6N2E2_386at 453.266 kb on - strand, within Pf6N2E2_386at 453.308 kb on + strand, within Pf6N2E2_386at 453.308 kb on + strand, within Pf6N2E2_386at 453.308 kb on + strand, within Pf6N2E2_386at 453.308 kb on + strand, within Pf6N2E2_386at 453.309 kb on - strand, within Pf6N2E2_386at 453.309 kb on - strand, within Pf6N2E2_386at 453.309 kb on - strand, within Pf6N2E2_386at 453.309 kb on - strand, within Pf6N2E2_386at 453.309 kb on - strand, within Pf6N2E2_386at 453.309 kb on - strand, within Pf6N2E2_386at 453.330 kb on + strand, within Pf6N2E2_386at 453.349 kb on - strand, within Pf6N2E2_386at 453.349 kb on - strand, within Pf6N2E2_386at 453.390 kb on - strand, within Pf6N2E2_386at 453.390 kb on - strand, within Pf6N2E2_386at 453.401 kb on - strand, within Pf6N2E2_386at 453.511 kb on + strand, within Pf6N2E2_386at 453.531 kb on + strand, within Pf6N2E2_386at 453.531 kb on + strand, within Pf6N2E2_386at 453.531 kb on + strand, within Pf6N2E2_386at 453.532 kb on - strand, within Pf6N2E2_386at 453.532 kb on - strand, within Pf6N2E2_386at 453.535 kb on + strand, within Pf6N2E2_386at 453.536 kb on - strand, within Pf6N2E2_386at 453.536 kb on - strand, within Pf6N2E2_386at 453.536 kb on - strand, within Pf6N2E2_386at 453.606 kb on - strand, within Pf6N2E2_386at 453.691 kb on + strand, within Pf6N2E2_386at 453.691 kb on + strand, within Pf6N2E2_386at 453.692 kb on - strand, within Pf6N2E2_386at 453.842 kb on - strand, within Pf6N2E2_386at 453.855 kb on + strand, within Pf6N2E2_386at 453.856 kb on - strand, within Pf6N2E2_386at 453.929 kb on + strandat 453.929 kb on + strandat 453.930 kb on - strandat 453.981 kb on + strandat 453.981 kb on + strandat 453.982 kb on - strandat 453.984 kb on - strandat 453.984 kb on - strandat 454.029 kb on - strandat 454.029 kb on - strandat 454.129 kb on - strand, within Pf6N2E2_387at 454.129 kb on - strand, within Pf6N2E2_387at 454.219 kb on - strand, within Pf6N2E2_387at 454.639 kb on + strand, within Pf6N2E2_387at 454.640 kb on - strand, within Pf6N2E2_387at 454.651 kb on + strand, within Pf6N2E2_387at 454.651 kb on + strand, within Pf6N2E2_387at 454.651 kb on + strand, within Pf6N2E2_387at 454.652 kb on - strand, within Pf6N2E2_387at 454.652 kb on - strand, within Pf6N2E2_387at 454.652 kb on - strand, within Pf6N2E2_387at 454.687 kb on + strand, within Pf6N2E2_387at 454.740 kb on + strand, within Pf6N2E2_387at 454.770 kb on + strand, within Pf6N2E2_387at 454.771 kb on - strand, within Pf6N2E2_387at 454.933 kb on + strand, within Pf6N2E2_387at 454.934 kb on - strand, within Pf6N2E2_387

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbon source D,L-Lactate 10 mM
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451,852 + +1.3
451,853 - Pf6N2E2_384 0.63 -0.0
451,853 - Pf6N2E2_384 0.63 +0.8
451,913 - Pf6N2E2_384 0.80 +0.8
452,005 + +0.3
452,005 + +0.8
452,006 - +0.7
452,019 + +0.8
452,019 + -0.9
452,020 - +0.2
452,020 - -0.8
452,021 + +0.8
452,021 + +0.8
452,022 - +0.8
452,022 - -0.3
452,264 + +0.8
452,405 + -0.7
452,635 - +0.1
452,635 - +0.8
452,637 - +0.7
452,678 - +0.0
452,678 - +0.8
452,679 + -0.8
452,758 - -0.2
452,819 - +0.1
452,819 - +0.4
452,919 + Pf6N2E2_386 0.17 +1.4
452,924 - Pf6N2E2_386 0.17 -0.5
452,931 - Pf6N2E2_386 0.17 -1.8
452,931 - Pf6N2E2_386 0.17 +0.2
452,931 - Pf6N2E2_386 0.17 -1.5
452,931 - Pf6N2E2_386 0.17 +1.4
452,960 + Pf6N2E2_386 0.20 +0.6
452,960 + Pf6N2E2_386 0.20 -0.2
452,960 + Pf6N2E2_386 0.20 -0.2
452,960 + Pf6N2E2_386 0.20 +0.6
452,961 - Pf6N2E2_386 0.20 -0.2
452,961 - Pf6N2E2_386 0.20 -1.2
452,964 - Pf6N2E2_386 0.20 -0.2
453,048 + Pf6N2E2_386 0.27 -0.2
453,049 - Pf6N2E2_386 0.27 -0.2
453,058 - Pf6N2E2_386 0.27 +0.8
453,058 - Pf6N2E2_386 0.27 +0.8
453,075 + Pf6N2E2_386 0.29 +1.6
453,200 - Pf6N2E2_386 0.38 -1.2
453,266 - Pf6N2E2_386 0.44 +0.8
453,308 + Pf6N2E2_386 0.47 +1.0
453,308 + Pf6N2E2_386 0.47 -0.8
453,308 + Pf6N2E2_386 0.47 +1.4
453,308 + Pf6N2E2_386 0.47 -0.8
453,309 - Pf6N2E2_386 0.47 +0.8
453,309 - Pf6N2E2_386 0.47 -0.8
453,309 - Pf6N2E2_386 0.47 -1.2
453,309 - Pf6N2E2_386 0.47 -0.2
453,309 - Pf6N2E2_386 0.47 -2.0
453,309 - Pf6N2E2_386 0.47 +0.0
453,330 + Pf6N2E2_386 0.49 +0.2
453,349 - Pf6N2E2_386 0.50 +0.8
453,349 - Pf6N2E2_386 0.50 -2.2
453,390 - Pf6N2E2_386 0.53 -0.8
453,390 - Pf6N2E2_386 0.53 -0.2
453,401 - Pf6N2E2_386 0.54 -1.2
453,511 + Pf6N2E2_386 0.63 +0.8
453,531 + Pf6N2E2_386 0.64 -0.2
453,531 + Pf6N2E2_386 0.64 -0.5
453,531 + Pf6N2E2_386 0.64 -0.0
453,532 - Pf6N2E2_386 0.64 +3.1
453,532 - Pf6N2E2_386 0.64 +0.8
453,535 + Pf6N2E2_386 0.65 -1.5
453,536 - Pf6N2E2_386 0.65 -0.2
453,536 - Pf6N2E2_386 0.65 -0.6
453,536 - Pf6N2E2_386 0.65 +0.8
453,606 - Pf6N2E2_386 0.70 -0.8
453,691 + Pf6N2E2_386 0.77 -0.2
453,691 + Pf6N2E2_386 0.77 -0.2
453,692 - Pf6N2E2_386 0.77 +0.5
453,842 - Pf6N2E2_386 0.89 +0.1
453,855 + Pf6N2E2_386 0.90 -0.8
453,856 - Pf6N2E2_386 0.90 -0.9
453,929 + -0.6
453,929 + -0.0
453,930 - -1.2
453,981 + +0.8
453,981 + +0.8
453,982 - +1.2
453,984 - -1.4
453,984 - -2.4
454,029 - -0.2
454,029 - +0.8
454,129 - Pf6N2E2_387 0.10 -0.5
454,129 - Pf6N2E2_387 0.10 -0.5
454,219 - Pf6N2E2_387 0.17 -2.2
454,639 + Pf6N2E2_387 0.47 -0.2
454,640 - Pf6N2E2_387 0.47 +0.9
454,651 + Pf6N2E2_387 0.48 -0.6
454,651 + Pf6N2E2_387 0.48 -1.1
454,651 + Pf6N2E2_387 0.48 -1.2
454,652 - Pf6N2E2_387 0.48 -0.0
454,652 - Pf6N2E2_387 0.48 +0.5
454,652 - Pf6N2E2_387 0.48 -0.2
454,687 + Pf6N2E2_387 0.50 -0.7
454,740 + Pf6N2E2_387 0.54 +1.4
454,770 + Pf6N2E2_387 0.56 +0.0
454,771 - Pf6N2E2_387 0.56 -0.6
454,933 + Pf6N2E2_387 0.68 +0.6
454,934 - Pf6N2E2_387 0.68 +0.8

Or see this region's nucleotide sequence