Experiment: Carbon source D,L-Lactate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2249 and Pf6N2E2_2250 are separated by 217 nucleotides Pf6N2E2_2250 and Pf6N2E2_2251 are separated by 10 nucleotides Pf6N2E2_2251 and Pf6N2E2_2252 are separated by 7 nucleotides
Pf6N2E2_2249 - Proton/glutamate symport protein @ Sodium/glutamate symport protein, at 2,663,256 to 2,664,518
_2249
Pf6N2E2_2250 - Transcriptional regulator, at 2,664,736 to 2,665,683
_2250
Pf6N2E2_2251 - NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3), at 2,665,694 to 2,666,578
_2251
Pf6N2E2_2252 - NreA-like protein, at 2,666,586 to 2,666,873
_2252
Position (kb)
2664
2665
2666 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2663.813 kb on - strand, within Pf6N2E2_2249 at 2663.857 kb on + strand, within Pf6N2E2_2249 at 2663.858 kb on - strand, within Pf6N2E2_2249 at 2663.858 kb on - strand, within Pf6N2E2_2249 at 2663.858 kb on - strand, within Pf6N2E2_2249 at 2663.882 kb on - strand, within Pf6N2E2_2249 at 2663.912 kb on + strand, within Pf6N2E2_2249 at 2663.938 kb on - strand, within Pf6N2E2_2249 at 2663.965 kb on + strand, within Pf6N2E2_2249 at 2663.965 kb on + strand, within Pf6N2E2_2249 at 2663.966 kb on - strand, within Pf6N2E2_2249 at 2664.335 kb on + strand, within Pf6N2E2_2249 at 2664.336 kb on - strand, within Pf6N2E2_2249 at 2664.345 kb on - strand, within Pf6N2E2_2249 at 2664.413 kb on + strand at 2664.413 kb on + strand at 2664.413 kb on + strand at 2664.414 kb on - strand at 2664.433 kb on + strand at 2664.498 kb on + strand at 2664.499 kb on - strand at 2664.539 kb on + strand at 2664.539 kb on + strand at 2664.540 kb on - strand at 2664.540 kb on - strand at 2664.540 kb on - strand at 2664.567 kb on + strand at 2664.568 kb on - strand at 2664.585 kb on + strand at 2664.585 kb on + strand at 2664.585 kb on + strand at 2664.604 kb on + strand at 2664.604 kb on + strand at 2664.604 kb on + strand at 2664.605 kb on - strand at 2664.605 kb on - strand at 2664.662 kb on + strand at 2664.662 kb on + strand at 2664.683 kb on - strand at 2664.892 kb on + strand, within Pf6N2E2_2250 at 2664.893 kb on - strand, within Pf6N2E2_2250 at 2664.893 kb on - strand, within Pf6N2E2_2250 at 2664.959 kb on + strand, within Pf6N2E2_2250 at 2665.119 kb on + strand, within Pf6N2E2_2250 at 2665.174 kb on + strand, within Pf6N2E2_2250 at 2665.175 kb on - strand, within Pf6N2E2_2250 at 2665.334 kb on - strand, within Pf6N2E2_2250 at 2665.415 kb on - strand, within Pf6N2E2_2250 at 2665.436 kb on + strand, within Pf6N2E2_2250 at 2665.437 kb on - strand, within Pf6N2E2_2250 at 2665.437 kb on - strand, within Pf6N2E2_2250 at 2665.437 kb on - strand, within Pf6N2E2_2250 at 2665.437 kb on - strand, within Pf6N2E2_2250 at 2665.571 kb on - strand, within Pf6N2E2_2250 at 2665.605 kb on + strand at 2665.606 kb on - strand at 2665.606 kb on - strand at 2665.695 kb on + strand at 2665.731 kb on + strand at 2665.731 kb on + strand at 2665.732 kb on - strand at 2665.754 kb on + strand at 2665.754 kb on + strand at 2665.754 kb on + strand at 2665.754 kb on + strand at 2665.755 kb on - strand at 2665.755 kb on - strand at 2665.755 kb on - strand at 2665.755 kb on - strand at 2665.755 kb on - strand at 2665.767 kb on + strand at 2665.767 kb on + strand at 2665.767 kb on + strand at 2665.767 kb on + strand at 2665.767 kb on + strand at 2665.767 kb on + strand at 2665.767 kb on + strand at 2665.767 kb on + strand at 2665.767 kb on + strand at 2665.767 kb on + strand at 2665.767 kb on + strand at 2665.767 kb on + strand at 2665.767 kb on + strand at 2665.768 kb on - strand at 2665.768 kb on - strand at 2665.768 kb on - strand at 2665.768 kb on - strand at 2665.768 kb on - strand at 2665.768 kb on - strand at 2665.768 kb on - strand at 2665.946 kb on - strand, within Pf6N2E2_2251 at 2666.249 kb on - strand, within Pf6N2E2_2251 at 2666.307 kb on + strand, within Pf6N2E2_2251 at 2666.308 kb on - strand, within Pf6N2E2_2251 at 2666.346 kb on + strand, within Pf6N2E2_2251 at 2666.347 kb on - strand, within Pf6N2E2_2251 at 2666.347 kb on - strand, within Pf6N2E2_2251 at 2666.347 kb on - strand, within Pf6N2E2_2251 at 2666.409 kb on - strand, within Pf6N2E2_2251 at 2666.409 kb on - strand, within Pf6N2E2_2251 at 2666.526 kb on + strand at 2666.527 kb on - strand at 2666.527 kb on - strand at 2666.527 kb on - strand at 2666.588 kb on - strand at 2666.588 kb on - strand at 2666.593 kb on + strand at 2666.593 kb on + strand at 2666.593 kb on + strand at 2666.593 kb on + strand at 2666.594 kb on - strand at 2666.594 kb on - strand at 2666.594 kb on - strand at 2666.594 kb on - strand at 2666.676 kb on + strand, within Pf6N2E2_2252 at 2666.677 kb on - strand, within Pf6N2E2_2252 at 2666.677 kb on - strand, within Pf6N2E2_2252 at 2666.677 kb on - strand, within Pf6N2E2_2252 at 2666.677 kb on - strand, within Pf6N2E2_2252 at 2666.677 kb on - strand, within Pf6N2E2_2252 at 2666.677 kb on - strand, within Pf6N2E2_2252 at 2666.677 kb on - strand, within Pf6N2E2_2252
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source D,L-Lactate 10 mM remove 2,663,813 - Pf6N2E2_2249 0.44 -0.3 2,663,857 + Pf6N2E2_2249 0.48 +0.6 2,663,858 - Pf6N2E2_2249 0.48 +0.6 2,663,858 - Pf6N2E2_2249 0.48 -0.7 2,663,858 - Pf6N2E2_2249 0.48 +0.1 2,663,882 - Pf6N2E2_2249 0.50 +0.9 2,663,912 + Pf6N2E2_2249 0.52 +0.0 2,663,938 - Pf6N2E2_2249 0.54 +0.4 2,663,965 + Pf6N2E2_2249 0.56 -0.4 2,663,965 + Pf6N2E2_2249 0.56 +0.3 2,663,966 - Pf6N2E2_2249 0.56 -0.1 2,664,335 + Pf6N2E2_2249 0.85 +0.3 2,664,336 - Pf6N2E2_2249 0.86 -0.1 2,664,345 - Pf6N2E2_2249 0.86 +0.6 2,664,413 + -0.1 2,664,413 + +0.9 2,664,413 + +0.9 2,664,414 - -1.4 2,664,433 + +0.9 2,664,498 + +1.9 2,664,499 - +1.5 2,664,539 + -1.4 2,664,539 + -0.3 2,664,540 - +0.3 2,664,540 - +0.1 2,664,540 - +0.7 2,664,567 + +0.9 2,664,568 - -1.1 2,664,585 + -0.5 2,664,585 + +0.9 2,664,585 + +0.9 2,664,604 + -0.2 2,664,604 + -0.5 2,664,604 + +0.2 2,664,605 - -0.1 2,664,605 - -0.1 2,664,662 + +0.9 2,664,662 + +0.5 2,664,683 - -0.1 2,664,892 + Pf6N2E2_2250 0.16 -0.1 2,664,893 - Pf6N2E2_2250 0.17 +0.9 2,664,893 - Pf6N2E2_2250 0.17 -1.1 2,664,959 + Pf6N2E2_2250 0.24 -0.7 2,665,119 + Pf6N2E2_2250 0.40 +0.2 2,665,174 + Pf6N2E2_2250 0.46 +0.9 2,665,175 - Pf6N2E2_2250 0.46 -1.3 2,665,334 - Pf6N2E2_2250 0.63 -0.5 2,665,415 - Pf6N2E2_2250 0.72 -1.9 2,665,436 + Pf6N2E2_2250 0.74 -0.1 2,665,437 - Pf6N2E2_2250 0.74 -0.6 2,665,437 - Pf6N2E2_2250 0.74 -0.3 2,665,437 - Pf6N2E2_2250 0.74 -0.5 2,665,437 - Pf6N2E2_2250 0.74 +0.3 2,665,571 - Pf6N2E2_2250 0.88 -0.4 2,665,605 + +0.3 2,665,606 - -0.4 2,665,606 - +0.4 2,665,695 + -0.4 2,665,731 + -1.1 2,665,731 + -0.5 2,665,732 - +0.6 2,665,754 + -0.1 2,665,754 + +0.4 2,665,754 + -0.6 2,665,754 + -0.1 2,665,755 - +0.9 2,665,755 - -0.7 2,665,755 - +0.9 2,665,755 - -0.7 2,665,755 - -0.1 2,665,767 + +0.1 2,665,767 + +0.9 2,665,767 + -1.1 2,665,767 + +0.5 2,665,767 + +0.1 2,665,767 + -1.9 2,665,767 + +0.9 2,665,767 + +0.2 2,665,767 + +1.5 2,665,767 + +1.0 2,665,767 + -1.1 2,665,767 + +0.4 2,665,767 + -1.1 2,665,768 - -0.1 2,665,768 - -0.1 2,665,768 - -0.7 2,665,768 - -0.1 2,665,768 - -0.3 2,665,768 - -0.7 2,665,768 - +1.2 2,665,946 - Pf6N2E2_2251 0.28 +2.2 2,666,249 - Pf6N2E2_2251 0.63 -0.3 2,666,307 + Pf6N2E2_2251 0.69 -0.1 2,666,308 - Pf6N2E2_2251 0.69 -0.1 2,666,346 + Pf6N2E2_2251 0.74 -0.7 2,666,347 - Pf6N2E2_2251 0.74 -0.2 2,666,347 - Pf6N2E2_2251 0.74 +0.4 2,666,347 - Pf6N2E2_2251 0.74 -0.8 2,666,409 - Pf6N2E2_2251 0.81 +0.4 2,666,409 - Pf6N2E2_2251 0.81 +0.9 2,666,526 + +0.6 2,666,527 - +1.1 2,666,527 - +1.3 2,666,527 - +0.5 2,666,588 - +0.9 2,666,588 - -1.5 2,666,593 + +1.7 2,666,593 + +0.7 2,666,593 + -1.2 2,666,593 + +0.3 2,666,594 - -0.9 2,666,594 - +0.3 2,666,594 - +0.5 2,666,594 - -0.7 2,666,676 + Pf6N2E2_2252 0.31 +0.9 2,666,677 - Pf6N2E2_2252 0.32 -1.1 2,666,677 - Pf6N2E2_2252 0.32 -0.4 2,666,677 - Pf6N2E2_2252 0.32 -1.9 2,666,677 - Pf6N2E2_2252 0.32 -0.2 2,666,677 - Pf6N2E2_2252 0.32 -0.9 2,666,677 - Pf6N2E2_2252 0.32 -0.7 2,666,677 - Pf6N2E2_2252 0.32 -0.7
Or see this region's nucleotide sequence