Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_618

Experiment: Carbon source Sucrose 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_616 and Pf6N2E2_617 are separated by 143 nucleotidesPf6N2E2_617 and Pf6N2E2_618 overlap by 4 nucleotidesPf6N2E2_618 and Pf6N2E2_619 overlap by 4 nucleotides Pf6N2E2_616 - hypothetical protein, at 699,541 to 699,666 _616 Pf6N2E2_617 - Probable glutathione S-transferase, at 699,810 to 700,412 _617 Pf6N2E2_618 - Exonuclease SbcC, at 700,409 to 704,050 _618 Pf6N2E2_619 - Exonuclease SbcD, at 704,047 to 705,291 _619 Position (kb) 700 701 702 703 704 705Strain fitness (log2 ratio) -2 -1 0 1 2 3at 699.527 kb on + strandat 699.527 kb on + strandat 699.528 kb on - strandat 699.578 kb on - strand, within Pf6N2E2_616at 699.812 kb on - strandat 700.076 kb on - strand, within Pf6N2E2_617at 700.076 kb on - strand, within Pf6N2E2_617at 700.081 kb on + strand, within Pf6N2E2_617at 700.081 kb on + strand, within Pf6N2E2_617at 700.082 kb on - strand, within Pf6N2E2_617at 700.394 kb on - strandat 700.394 kb on - strandat 700.394 kb on - strandat 700.559 kb on + strandat 700.697 kb on + strandat 700.697 kb on + strandat 700.697 kb on + strandat 700.698 kb on - strandat 700.771 kb on + strandat 700.771 kb on + strandat 700.771 kb on + strandat 700.772 kb on - strandat 700.772 kb on - strandat 700.782 kb on - strand, within Pf6N2E2_618at 700.833 kb on + strand, within Pf6N2E2_618at 700.908 kb on + strand, within Pf6N2E2_618at 700.917 kb on + strand, within Pf6N2E2_618at 700.917 kb on + strand, within Pf6N2E2_618at 700.917 kb on + strand, within Pf6N2E2_618at 700.917 kb on + strand, within Pf6N2E2_618at 700.917 kb on + strand, within Pf6N2E2_618at 700.918 kb on - strand, within Pf6N2E2_618at 700.918 kb on - strand, within Pf6N2E2_618at 700.918 kb on - strand, within Pf6N2E2_618at 700.991 kb on + strand, within Pf6N2E2_618at 700.991 kb on + strand, within Pf6N2E2_618at 701.345 kb on + strand, within Pf6N2E2_618at 701.346 kb on - strand, within Pf6N2E2_618at 701.346 kb on - strand, within Pf6N2E2_618at 702.090 kb on + strand, within Pf6N2E2_618at 702.090 kb on + strand, within Pf6N2E2_618at 702.207 kb on + strand, within Pf6N2E2_618at 702.579 kb on + strand, within Pf6N2E2_618at 702.580 kb on - strand, within Pf6N2E2_618at 703.244 kb on + strand, within Pf6N2E2_618at 703.461 kb on + strand, within Pf6N2E2_618at 703.462 kb on - strand, within Pf6N2E2_618at 703.611 kb on + strand, within Pf6N2E2_618at 704.130 kb on - strandat 704.234 kb on + strand, within Pf6N2E2_619at 704.624 kb on + strand, within Pf6N2E2_619at 704.624 kb on + strand, within Pf6N2E2_619at 704.625 kb on - strand, within Pf6N2E2_619at 704.770 kb on - strand, within Pf6N2E2_619

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbon source Sucrose 5 mM
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699,527 + +2.0
699,527 + +0.1
699,528 - -0.0
699,578 - Pf6N2E2_616 0.29 +0.0
699,812 - -0.5
700,076 - Pf6N2E2_617 0.44 -2.5
700,076 - Pf6N2E2_617 0.44 +1.8
700,081 + Pf6N2E2_617 0.45 -0.2
700,081 + Pf6N2E2_617 0.45 -0.5
700,082 - Pf6N2E2_617 0.45 +2.6
700,394 - -0.3
700,394 - -1.5
700,394 - +1.2
700,559 + +0.0
700,697 + +1.0
700,697 + -0.3
700,697 + -1.0
700,698 - -0.5
700,771 + -0.2
700,771 + -1.0
700,771 + -0.5
700,772 - -2.2
700,772 - -0.3
700,782 - Pf6N2E2_618 0.10 +0.0
700,833 + Pf6N2E2_618 0.12 +0.3
700,908 + Pf6N2E2_618 0.14 +1.0
700,917 + Pf6N2E2_618 0.14 -1.4
700,917 + Pf6N2E2_618 0.14 +0.0
700,917 + Pf6N2E2_618 0.14 +2.6
700,917 + Pf6N2E2_618 0.14 -1.3
700,917 + Pf6N2E2_618 0.14 +2.0
700,918 - Pf6N2E2_618 0.14 +3.0
700,918 - Pf6N2E2_618 0.14 +1.2
700,918 - Pf6N2E2_618 0.14 +0.6
700,991 + Pf6N2E2_618 0.16 -0.2
700,991 + Pf6N2E2_618 0.16 +0.2
701,345 + Pf6N2E2_618 0.26 -1.3
701,346 - Pf6N2E2_618 0.26 +1.0
701,346 - Pf6N2E2_618 0.26 +1.0
702,090 + Pf6N2E2_618 0.46 -1.8
702,090 + Pf6N2E2_618 0.46 +0.1
702,207 + Pf6N2E2_618 0.49 +1.0
702,579 + Pf6N2E2_618 0.60 -1.8
702,580 - Pf6N2E2_618 0.60 +0.5
703,244 + Pf6N2E2_618 0.78 +1.0
703,461 + Pf6N2E2_618 0.84 +0.7
703,462 - Pf6N2E2_618 0.84 +0.9
703,611 + Pf6N2E2_618 0.88 +0.6
704,130 - +1.0
704,234 + Pf6N2E2_619 0.15 -0.2
704,624 + Pf6N2E2_619 0.46 +0.3
704,624 + Pf6N2E2_619 0.46 +1.0
704,625 - Pf6N2E2_619 0.46 -0.8
704,770 - Pf6N2E2_619 0.58 -0.5

Or see this region's nucleotide sequence