Experiment: Carbon source Sucrose 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5390 and Pf6N2E2_5391 are separated by 336 nucleotides Pf6N2E2_5391 and Pf6N2E2_5392 are separated by 168 nucleotides Pf6N2E2_5392 and Pf6N2E2_5393 overlap by 4 nucleotides Pf6N2E2_5393 and Pf6N2E2_5394 are separated by 3 nucleotides
Pf6N2E2_5390 - Uricase (urate oxidase) (EC 1.7.3.3), at 6,196,445 to 6,197,575
_5390
Pf6N2E2_5391 - Predicted ATPase related to phosphate starvation-inducible protein PhoH, at 6,197,912 to 6,199,306
_5391
Pf6N2E2_5392 - Molybdenum cofactor biosynthesis protein MoaC, at 6,199,475 to 6,199,948
_5392
Pf6N2E2_5393 - Molybdenum cofactor biosynthesis protein MoaD, at 6,199,945 to 6,200,187
_5393
Pf6N2E2_5394 - Molybdenum cofactor biosynthesis protein MoaE, at 6,200,191 to 6,200,643
_5394
Position (kb)
6197
6198
6199
6200 Strain fitness (log2 ratio)
-2
-1
0
1 at 6197.064 kb on - strand, within Pf6N2E2_5390 at 6197.162 kb on + strand, within Pf6N2E2_5390 at 6197.162 kb on + strand, within Pf6N2E2_5390 at 6197.162 kb on + strand, within Pf6N2E2_5390 at 6197.162 kb on + strand, within Pf6N2E2_5390 at 6197.163 kb on - strand, within Pf6N2E2_5390 at 6197.163 kb on - strand, within Pf6N2E2_5390 at 6197.163 kb on - strand, within Pf6N2E2_5390 at 6197.213 kb on + strand, within Pf6N2E2_5390 at 6197.213 kb on + strand, within Pf6N2E2_5390 at 6197.213 kb on + strand, within Pf6N2E2_5390 at 6197.282 kb on + strand, within Pf6N2E2_5390 at 6197.624 kb on + strand at 6197.625 kb on - strand at 6197.681 kb on - strand at 6197.699 kb on + strand at 6197.749 kb on + strand at 6197.749 kb on + strand at 6197.750 kb on - strand at 6197.750 kb on - strand at 6197.899 kb on + strand at 6197.899 kb on + strand at 6197.899 kb on + strand at 6197.899 kb on + strand at 6197.899 kb on + strand at 6197.899 kb on + strand at 6197.899 kb on + strand at 6197.899 kb on + strand at 6197.899 kb on + strand at 6197.899 kb on + strand at 6197.899 kb on + strand at 6197.899 kb on + strand at 6197.899 kb on + strand at 6197.899 kb on + strand at 6197.899 kb on + strand at 6197.900 kb on - strand at 6197.900 kb on - strand at 6197.900 kb on - strand at 6197.900 kb on - strand at 6197.900 kb on - strand at 6197.912 kb on - strand at 6197.929 kb on + strand at 6197.930 kb on - strand at 6197.957 kb on + strand at 6197.957 kb on + strand at 6197.958 kb on - strand at 6197.958 kb on - strand at 6197.958 kb on - strand at 6197.958 kb on - strand at 6197.958 kb on - strand at 6197.961 kb on + strand at 6197.961 kb on + strand at 6197.961 kb on + strand at 6197.961 kb on + strand at 6197.961 kb on + strand at 6197.961 kb on + strand at 6197.962 kb on - strand at 6197.962 kb on - strand at 6197.962 kb on - strand at 6197.962 kb on - strand at 6197.976 kb on + strand at 6197.976 kb on + strand at 6197.976 kb on + strand at 6197.976 kb on + strand at 6197.977 kb on - strand at 6197.977 kb on - strand at 6197.977 kb on - strand at 6198.181 kb on + strand, within Pf6N2E2_5391 at 6198.181 kb on + strand, within Pf6N2E2_5391 at 6198.181 kb on + strand, within Pf6N2E2_5391 at 6198.181 kb on + strand, within Pf6N2E2_5391 at 6198.182 kb on - strand, within Pf6N2E2_5391 at 6198.389 kb on + strand, within Pf6N2E2_5391 at 6198.389 kb on + strand, within Pf6N2E2_5391 at 6198.390 kb on - strand, within Pf6N2E2_5391 at 6198.689 kb on + strand, within Pf6N2E2_5391 at 6198.689 kb on + strand, within Pf6N2E2_5391 at 6198.690 kb on - strand, within Pf6N2E2_5391 at 6198.690 kb on - strand, within Pf6N2E2_5391 at 6198.690 kb on - strand, within Pf6N2E2_5391 at 6198.690 kb on - strand, within Pf6N2E2_5391 at 6198.690 kb on - strand, within Pf6N2E2_5391 at 6198.690 kb on - strand, within Pf6N2E2_5391 at 6198.983 kb on + strand, within Pf6N2E2_5391 at 6198.983 kb on + strand, within Pf6N2E2_5391 at 6198.983 kb on + strand, within Pf6N2E2_5391 at 6198.983 kb on + strand, within Pf6N2E2_5391 at 6198.983 kb on + strand, within Pf6N2E2_5391 at 6198.983 kb on + strand, within Pf6N2E2_5391 at 6198.984 kb on - strand, within Pf6N2E2_5391 at 6198.984 kb on - strand, within Pf6N2E2_5391 at 6198.984 kb on - strand, within Pf6N2E2_5391 at 6198.984 kb on - strand, within Pf6N2E2_5391 at 6198.984 kb on - strand, within Pf6N2E2_5391 at 6198.984 kb on - strand, within Pf6N2E2_5391 at 6199.025 kb on + strand, within Pf6N2E2_5391 at 6199.025 kb on + strand, within Pf6N2E2_5391 at 6199.025 kb on + strand, within Pf6N2E2_5391 at 6199.026 kb on - strand, within Pf6N2E2_5391 at 6199.026 kb on - strand, within Pf6N2E2_5391 at 6199.026 kb on - strand, within Pf6N2E2_5391 at 6199.026 kb on - strand, within Pf6N2E2_5391 at 6199.026 kb on - strand, within Pf6N2E2_5391 at 6199.026 kb on - strand, within Pf6N2E2_5391 at 6199.168 kb on + strand at 6199.175 kb on + strand at 6199.175 kb on + strand at 6199.175 kb on + strand at 6199.175 kb on + strand at 6199.175 kb on + strand at 6199.175 kb on + strand at 6199.175 kb on + strand at 6199.176 kb on - strand at 6199.176 kb on - strand at 6199.176 kb on - strand at 6199.176 kb on - strand at 6199.176 kb on - strand at 6199.205 kb on + strand at 6199.205 kb on + strand at 6199.205 kb on + strand at 6199.205 kb on + strand at 6199.206 kb on - strand at 6199.206 kb on - strand at 6199.332 kb on - strand at 6199.347 kb on - strand at 6199.347 kb on - strand at 6199.347 kb on - strand at 6199.347 kb on - strand at 6199.347 kb on - strand at 6199.353 kb on + strand at 6199.353 kb on + strand at 6199.354 kb on - strand at 6199.354 kb on - strand at 6199.354 kb on - strand at 6199.435 kb on + strand at 6199.435 kb on + strand at 6199.443 kb on + strand at 6199.443 kb on + strand at 6199.444 kb on - strand at 6199.469 kb on - strand at 6199.755 kb on + strand, within Pf6N2E2_5392 at 6199.844 kb on + strand, within Pf6N2E2_5392 at 6199.844 kb on + strand, within Pf6N2E2_5392 at 6199.844 kb on + strand, within Pf6N2E2_5392 at 6199.845 kb on - strand, within Pf6N2E2_5392 at 6199.845 kb on - strand, within Pf6N2E2_5392
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 5 mM remove 6,197,064 - Pf6N2E2_5390 0.55 -2.0 6,197,162 + Pf6N2E2_5390 0.63 -0.4 6,197,162 + Pf6N2E2_5390 0.63 -0.1 6,197,162 + Pf6N2E2_5390 0.63 -1.6 6,197,162 + Pf6N2E2_5390 0.63 +0.5 6,197,163 - Pf6N2E2_5390 0.63 +0.6 6,197,163 - Pf6N2E2_5390 0.63 +0.3 6,197,163 - Pf6N2E2_5390 0.63 +0.8 6,197,213 + Pf6N2E2_5390 0.68 +0.7 6,197,213 + Pf6N2E2_5390 0.68 -0.2 6,197,213 + Pf6N2E2_5390 0.68 -1.4 6,197,282 + Pf6N2E2_5390 0.74 -0.4 6,197,624 + +0.1 6,197,625 - -1.2 6,197,681 - +1.6 6,197,699 + +0.0 6,197,749 + -1.7 6,197,749 + -0.4 6,197,750 - +1.3 6,197,750 - -0.5 6,197,899 + +1.6 6,197,899 + -1.6 6,197,899 + +1.6 6,197,899 + +0.6 6,197,899 + -1.0 6,197,899 + -0.6 6,197,899 + +1.6 6,197,899 + -0.5 6,197,899 + -1.2 6,197,899 + +0.0 6,197,899 + +0.6 6,197,899 + -1.4 6,197,899 + -1.4 6,197,899 + +0.0 6,197,899 + +0.0 6,197,900 - -1.4 6,197,900 - +1.6 6,197,900 - -1.5 6,197,900 - +0.0 6,197,900 - -2.1 6,197,912 - -1.8 6,197,929 + -0.5 6,197,930 - -2.5 6,197,957 + +0.6 6,197,957 + +0.0 6,197,958 - +0.6 6,197,958 - -0.7 6,197,958 - +0.8 6,197,958 - -0.6 6,197,958 - +1.6 6,197,961 + -0.4 6,197,961 + +0.6 6,197,961 + -0.4 6,197,961 + -0.7 6,197,961 + -1.3 6,197,961 + -1.9 6,197,962 - +0.0 6,197,962 - +1.6 6,197,962 - -1.7 6,197,962 - -1.2 6,197,976 + +0.0 6,197,976 + +0.0 6,197,976 + -0.4 6,197,976 + -1.5 6,197,977 - -0.4 6,197,977 - +0.6 6,197,977 - +0.8 6,198,181 + Pf6N2E2_5391 0.19 -0.7 6,198,181 + Pf6N2E2_5391 0.19 +1.6 6,198,181 + Pf6N2E2_5391 0.19 -0.4 6,198,181 + Pf6N2E2_5391 0.19 +1.6 6,198,182 - Pf6N2E2_5391 0.19 -0.7 6,198,389 + Pf6N2E2_5391 0.34 +0.6 6,198,389 + Pf6N2E2_5391 0.34 +1.6 6,198,390 - Pf6N2E2_5391 0.34 -1.7 6,198,689 + Pf6N2E2_5391 0.56 -0.5 6,198,689 + Pf6N2E2_5391 0.56 +1.6 6,198,690 - Pf6N2E2_5391 0.56 +0.6 6,198,690 - Pf6N2E2_5391 0.56 -0.5 6,198,690 - Pf6N2E2_5391 0.56 -2.0 6,198,690 - Pf6N2E2_5391 0.56 -1.6 6,198,690 - Pf6N2E2_5391 0.56 -1.4 6,198,690 - Pf6N2E2_5391 0.56 +0.0 6,198,983 + Pf6N2E2_5391 0.77 -0.4 6,198,983 + Pf6N2E2_5391 0.77 -0.1 6,198,983 + Pf6N2E2_5391 0.77 -1.5 6,198,983 + Pf6N2E2_5391 0.77 +0.3 6,198,983 + Pf6N2E2_5391 0.77 -1.0 6,198,983 + Pf6N2E2_5391 0.77 +0.0 6,198,984 - Pf6N2E2_5391 0.77 -2.1 6,198,984 - Pf6N2E2_5391 0.77 -1.5 6,198,984 - Pf6N2E2_5391 0.77 +0.6 6,198,984 - Pf6N2E2_5391 0.77 -1.2 6,198,984 - Pf6N2E2_5391 0.77 -0.8 6,198,984 - Pf6N2E2_5391 0.77 +0.0 6,199,025 + Pf6N2E2_5391 0.80 -1.1 6,199,025 + Pf6N2E2_5391 0.80 -1.8 6,199,025 + Pf6N2E2_5391 0.80 -1.3 6,199,026 - Pf6N2E2_5391 0.80 -1.8 6,199,026 - Pf6N2E2_5391 0.80 -1.5 6,199,026 - Pf6N2E2_5391 0.80 +0.2 6,199,026 - Pf6N2E2_5391 0.80 -0.7 6,199,026 - Pf6N2E2_5391 0.80 -2.5 6,199,026 - Pf6N2E2_5391 0.80 -2.3 6,199,168 + +0.2 6,199,175 + -2.2 6,199,175 + -0.3 6,199,175 + -0.5 6,199,175 + -1.1 6,199,175 + +0.6 6,199,175 + +0.2 6,199,175 + -1.4 6,199,176 - +0.0 6,199,176 - -1.4 6,199,176 - -1.0 6,199,176 - -0.6 6,199,176 - +0.5 6,199,205 + +1.6 6,199,205 + +1.6 6,199,205 + -1.1 6,199,205 + -1.4 6,199,206 - -0.7 6,199,206 - -0.1 6,199,332 - -1.1 6,199,347 - -1.4 6,199,347 - -0.0 6,199,347 - -0.1 6,199,347 - -1.0 6,199,347 - +0.3 6,199,353 + -0.2 6,199,353 + -1.7 6,199,354 - -0.2 6,199,354 - +0.9 6,199,354 - -0.5 6,199,435 + +0.4 6,199,435 + +0.0 6,199,443 + -1.7 6,199,443 + -0.9 6,199,444 - +1.0 6,199,469 - -0.4 6,199,755 + Pf6N2E2_5392 0.59 -2.5 6,199,844 + Pf6N2E2_5392 0.78 +0.0 6,199,844 + Pf6N2E2_5392 0.78 -1.0 6,199,844 + Pf6N2E2_5392 0.78 +0.2 6,199,845 - Pf6N2E2_5392 0.78 +0.9 6,199,845 - Pf6N2E2_5392 0.78 +1.3
Or see this region's nucleotide sequence