Experiment: Carbon source Sucrose 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4963 and Pf6N2E2_4964 overlap by 4 nucleotides
Pf6N2E2_4963 - Homoserine dehydrogenase (EC 1.1.1.3), at 5,724,211 to 5,725,269
_4963
Pf6N2E2_4964 - Aldehyde dehydrogenase (EC 1.2.1.3), at 5,725,266 to 5,726,678
_4964
Position (kb)
5724
5725
5726 Strain fitness (log2 ratio)
-1
0
1
2 at 5723.499 kb on + strand at 5723.499 kb on + strand at 5723.818 kb on + strand at 5723.818 kb on + strand at 5723.989 kb on + strand at 5723.989 kb on + strand at 5723.989 kb on + strand at 5723.989 kb on + strand at 5723.989 kb on + strand at 5723.989 kb on + strand at 5723.989 kb on + strand at 5723.990 kb on - strand at 5723.990 kb on - strand at 5723.990 kb on - strand at 5723.990 kb on - strand at 5724.009 kb on + strand at 5724.009 kb on + strand at 5724.009 kb on + strand at 5724.010 kb on - strand at 5724.010 kb on - strand at 5724.010 kb on - strand at 5724.010 kb on - strand at 5724.010 kb on - strand at 5724.084 kb on - strand at 5724.123 kb on - strand at 5724.138 kb on - strand at 5724.138 kb on - strand at 5724.167 kb on + strand at 5724.167 kb on + strand at 5724.167 kb on + strand at 5724.167 kb on + strand at 5724.167 kb on + strand at 5724.168 kb on - strand at 5724.168 kb on - strand at 5724.204 kb on - strand at 5724.220 kb on + strand at 5724.220 kb on + strand at 5724.220 kb on + strand at 5724.221 kb on - strand at 5724.589 kb on - strand, within Pf6N2E2_4963 at 5724.662 kb on - strand, within Pf6N2E2_4963 at 5725.408 kb on + strand, within Pf6N2E2_4964 at 5725.409 kb on - strand, within Pf6N2E2_4964 at 5725.409 kb on - strand, within Pf6N2E2_4964 at 5725.705 kb on - strand, within Pf6N2E2_4964 at 5725.705 kb on - strand, within Pf6N2E2_4964 at 5726.133 kb on + strand, within Pf6N2E2_4964 at 5726.133 kb on + strand, within Pf6N2E2_4964 at 5726.133 kb on + strand, within Pf6N2E2_4964 at 5726.134 kb on - strand, within Pf6N2E2_4964 at 5726.134 kb on - strand, within Pf6N2E2_4964
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 5 mM remove 5,723,499 + +0.8 5,723,499 + +1.3 5,723,818 + -0.5 5,723,818 + -1.1 5,723,989 + -0.5 5,723,989 + +1.8 5,723,989 + +0.4 5,723,989 + +1.8 5,723,989 + +0.5 5,723,989 + +0.4 5,723,989 + +0.8 5,723,990 - +1.4 5,723,990 - -0.7 5,723,990 - +0.9 5,723,990 - +0.4 5,724,009 + -0.8 5,724,009 + +1.8 5,724,009 + -0.5 5,724,010 - -0.8 5,724,010 - -1.4 5,724,010 - +0.4 5,724,010 - -0.2 5,724,010 - -0.3 5,724,084 - -0.2 5,724,123 - +0.9 5,724,138 - +0.5 5,724,138 - +1.2 5,724,167 + -0.6 5,724,167 + -0.3 5,724,167 + -1.0 5,724,167 + -0.5 5,724,167 + +1.2 5,724,168 - -0.4 5,724,168 - +1.8 5,724,204 - +1.8 5,724,220 + -0.4 5,724,220 + -0.5 5,724,220 + -0.5 5,724,221 - -0.3 5,724,589 - Pf6N2E2_4963 0.36 -1.2 5,724,662 - Pf6N2E2_4963 0.43 -0.2 5,725,408 + Pf6N2E2_4964 0.10 -0.5 5,725,409 - Pf6N2E2_4964 0.10 -0.2 5,725,409 - Pf6N2E2_4964 0.10 -0.5 5,725,705 - Pf6N2E2_4964 0.31 -0.5 5,725,705 - Pf6N2E2_4964 0.31 +0.8 5,726,133 + Pf6N2E2_4964 0.61 +0.4 5,726,133 + Pf6N2E2_4964 0.61 -0.1 5,726,133 + Pf6N2E2_4964 0.61 -1.0 5,726,134 - Pf6N2E2_4964 0.61 +0.8 5,726,134 - Pf6N2E2_4964 0.61 +0.2
Or see this region's nucleotide sequence