Experiment: Carbon source Sucrose 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2895 and Pf6N2E2_2896 overlap by 11 nucleotides Pf6N2E2_2896 and Pf6N2E2_2897 are separated by 184 nucleotides Pf6N2E2_2897 and Pf6N2E2_2898 overlap by 4 nucleotides
Pf6N2E2_2895 - Integral membrane sensor signal transduction histidine kinase (EC 2.7.13.3), glucose catabolism cluster, at 3,383,555 to 3,385,024
_2895
Pf6N2E2_2896 - DNA-binding response regulator GltR, controls specific porins for the entry of glucose, at 3,385,014 to 3,385,745
_2896
Pf6N2E2_2897 - Glucokinase (EC 2.7.1.2), at 3,385,930 to 3,386,889
_2897
Pf6N2E2_2898 - Phosphogluconate dehydratase (EC 4.2.1.12), at 3,386,886 to 3,388,712
_2898
Position (kb)
3385
3386
3387 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3385.432 kb on + strand, within Pf6N2E2_2896 at 3385.432 kb on + strand, within Pf6N2E2_2896 at 3385.432 kb on + strand, within Pf6N2E2_2896 at 3385.432 kb on + strand, within Pf6N2E2_2896 at 3385.433 kb on - strand, within Pf6N2E2_2896 at 3385.545 kb on + strand, within Pf6N2E2_2896 at 3385.545 kb on + strand, within Pf6N2E2_2896 at 3385.675 kb on + strand at 3385.675 kb on + strand at 3385.675 kb on + strand at 3385.675 kb on + strand at 3385.675 kb on + strand at 3385.675 kb on + strand at 3385.675 kb on + strand at 3385.676 kb on - strand at 3385.676 kb on - strand at 3385.779 kb on + strand at 3385.780 kb on - strand at 3385.799 kb on + strand at 3385.800 kb on - strand at 3385.800 kb on - strand at 3385.810 kb on + strand at 3385.811 kb on - strand at 3385.913 kb on - strand at 3385.913 kb on - strand at 3385.913 kb on - strand at 3385.922 kb on - strand at 3386.022 kb on - strand at 3386.114 kb on + strand, within Pf6N2E2_2897 at 3386.114 kb on + strand, within Pf6N2E2_2897 at 3386.114 kb on + strand, within Pf6N2E2_2897 at 3386.114 kb on + strand, within Pf6N2E2_2897 at 3386.114 kb on + strand, within Pf6N2E2_2897 at 3386.114 kb on + strand, within Pf6N2E2_2897 at 3386.115 kb on - strand, within Pf6N2E2_2897 at 3386.115 kb on - strand, within Pf6N2E2_2897 at 3386.115 kb on - strand, within Pf6N2E2_2897 at 3386.115 kb on - strand, within Pf6N2E2_2897 at 3386.354 kb on + strand, within Pf6N2E2_2897 at 3386.354 kb on + strand, within Pf6N2E2_2897 at 3386.354 kb on + strand, within Pf6N2E2_2897 at 3386.354 kb on + strand, within Pf6N2E2_2897 at 3386.354 kb on + strand, within Pf6N2E2_2897 at 3386.354 kb on + strand, within Pf6N2E2_2897 at 3386.355 kb on - strand, within Pf6N2E2_2897 at 3386.355 kb on - strand, within Pf6N2E2_2897 at 3386.463 kb on + strand, within Pf6N2E2_2897 at 3386.463 kb on + strand, within Pf6N2E2_2897 at 3386.464 kb on - strand, within Pf6N2E2_2897 at 3386.464 kb on - strand, within Pf6N2E2_2897 at 3386.464 kb on - strand, within Pf6N2E2_2897 at 3386.464 kb on - strand, within Pf6N2E2_2897 at 3386.464 kb on - strand, within Pf6N2E2_2897 at 3386.533 kb on + strand, within Pf6N2E2_2897 at 3386.533 kb on + strand, within Pf6N2E2_2897 at 3386.667 kb on + strand, within Pf6N2E2_2897 at 3386.756 kb on + strand, within Pf6N2E2_2897 at 3386.757 kb on - strand, within Pf6N2E2_2897 at 3386.786 kb on + strand, within Pf6N2E2_2897 at 3386.787 kb on - strand, within Pf6N2E2_2897 at 3386.892 kb on - strand at 3386.892 kb on - strand at 3386.892 kb on - strand at 3386.892 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 5 mM remove 3,385,432 + Pf6N2E2_2896 0.57 -0.4 3,385,432 + Pf6N2E2_2896 0.57 -1.6 3,385,432 + Pf6N2E2_2896 0.57 -0.1 3,385,432 + Pf6N2E2_2896 0.57 +0.6 3,385,433 - Pf6N2E2_2896 0.57 +0.6 3,385,545 + Pf6N2E2_2896 0.73 -0.8 3,385,545 + Pf6N2E2_2896 0.73 -0.0 3,385,675 + +0.0 3,385,675 + -1.2 3,385,675 + +0.8 3,385,675 + -0.0 3,385,675 + -2.0 3,385,675 + -0.3 3,385,675 + +0.6 3,385,676 - +0.5 3,385,676 - -2.0 3,385,779 + -0.0 3,385,780 - +1.3 3,385,799 + -1.0 3,385,800 - +0.4 3,385,800 - -0.4 3,385,810 + -2.5 3,385,811 - -0.0 3,385,913 - -0.7 3,385,913 - -0.0 3,385,913 - +1.0 3,385,922 - -0.2 3,386,022 - -1.2 3,386,114 + Pf6N2E2_2897 0.19 -0.4 3,386,114 + Pf6N2E2_2897 0.19 -2.8 3,386,114 + Pf6N2E2_2897 0.19 -1.5 3,386,114 + Pf6N2E2_2897 0.19 +0.6 3,386,114 + Pf6N2E2_2897 0.19 -1.5 3,386,114 + Pf6N2E2_2897 0.19 -3.3 3,386,115 - Pf6N2E2_2897 0.19 -4.6 3,386,115 - Pf6N2E2_2897 0.19 -4.1 3,386,115 - Pf6N2E2_2897 0.19 -4.4 3,386,115 - Pf6N2E2_2897 0.19 -4.3 3,386,354 + Pf6N2E2_2897 0.44 +0.6 3,386,354 + Pf6N2E2_2897 0.44 -1.0 3,386,354 + Pf6N2E2_2897 0.44 -4.1 3,386,354 + Pf6N2E2_2897 0.44 -2.4 3,386,354 + Pf6N2E2_2897 0.44 -2.3 3,386,354 + Pf6N2E2_2897 0.44 +0.6 3,386,355 - Pf6N2E2_2897 0.44 -2.2 3,386,355 - Pf6N2E2_2897 0.44 -2.3 3,386,463 + Pf6N2E2_2897 0.56 +1.6 3,386,463 + Pf6N2E2_2897 0.56 -4.5 3,386,464 - Pf6N2E2_2897 0.56 -0.0 3,386,464 - Pf6N2E2_2897 0.56 -0.0 3,386,464 - Pf6N2E2_2897 0.56 -2.4 3,386,464 - Pf6N2E2_2897 0.56 -1.2 3,386,464 - Pf6N2E2_2897 0.56 -3.4 3,386,533 + Pf6N2E2_2897 0.63 +1.6 3,386,533 + Pf6N2E2_2897 0.63 +1.6 3,386,667 + Pf6N2E2_2897 0.77 -0.0 3,386,756 + Pf6N2E2_2897 0.86 -1.9 3,386,757 - Pf6N2E2_2897 0.86 -1.6 3,386,786 + Pf6N2E2_2897 0.89 +1.6 3,386,787 - Pf6N2E2_2897 0.89 -4.1 3,386,892 - -2.0 3,386,892 - -3.1 3,386,892 - -4.8 3,386,892 - -3.8
Or see this region's nucleotide sequence