Experiment: Carbon source Sucrose 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2301 and Pf6N2E2_2302 are separated by 361 nucleotides Pf6N2E2_2302 and Pf6N2E2_2303 are separated by 86 nucleotides
Pf6N2E2_2301 - Aconitate hydratase (EC 4.2.1.3), at 2,724,105 to 2,726,846
_2301
Pf6N2E2_2302 - CAAX amino terminal protease family protein, at 2,727,208 to 2,727,996
_2302
Pf6N2E2_2303 - Aerotaxis receptor Aer, at 2,728,083 to 2,729,648
_2303
Position (kb)
2727
2728 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2726.277 kb on + strand, within Pf6N2E2_2301 at 2726.382 kb on + strand, within Pf6N2E2_2301 at 2726.382 kb on + strand, within Pf6N2E2_2301 at 2726.382 kb on + strand, within Pf6N2E2_2301 at 2726.382 kb on + strand, within Pf6N2E2_2301 at 2726.382 kb on + strand, within Pf6N2E2_2301 at 2726.382 kb on + strand, within Pf6N2E2_2301 at 2726.382 kb on + strand, within Pf6N2E2_2301 at 2726.383 kb on - strand, within Pf6N2E2_2301 at 2726.383 kb on - strand, within Pf6N2E2_2301 at 2726.383 kb on - strand, within Pf6N2E2_2301 at 2726.383 kb on - strand, within Pf6N2E2_2301 at 2726.383 kb on - strand, within Pf6N2E2_2301 at 2726.383 kb on - strand, within Pf6N2E2_2301 at 2726.383 kb on - strand, within Pf6N2E2_2301 at 2726.383 kb on - strand, within Pf6N2E2_2301 at 2726.412 kb on + strand, within Pf6N2E2_2301 at 2726.412 kb on + strand, within Pf6N2E2_2301 at 2726.412 kb on + strand, within Pf6N2E2_2301 at 2726.430 kb on + strand, within Pf6N2E2_2301 at 2726.430 kb on + strand, within Pf6N2E2_2301 at 2726.430 kb on + strand, within Pf6N2E2_2301 at 2726.430 kb on + strand, within Pf6N2E2_2301 at 2726.430 kb on + strand, within Pf6N2E2_2301 at 2726.431 kb on - strand, within Pf6N2E2_2301 at 2726.476 kb on - strand, within Pf6N2E2_2301 at 2726.562 kb on - strand, within Pf6N2E2_2301 at 2726.771 kb on + strand at 2726.790 kb on + strand at 2726.791 kb on - strand at 2726.791 kb on - strand at 2726.791 kb on - strand at 2726.791 kb on - strand at 2726.817 kb on + strand at 2726.817 kb on + strand at 2726.817 kb on + strand at 2726.817 kb on + strand at 2726.817 kb on + strand at 2726.817 kb on + strand at 2726.817 kb on + strand at 2726.818 kb on - strand at 2726.818 kb on - strand at 2726.818 kb on - strand at 2726.818 kb on - strand at 2726.818 kb on - strand at 2726.818 kb on - strand at 2726.818 kb on - strand at 2726.818 kb on - strand at 2726.818 kb on - strand at 2726.844 kb on + strand at 2726.844 kb on + strand at 2726.844 kb on + strand at 2726.861 kb on + strand at 2726.861 kb on + strand at 2726.925 kb on - strand at 2726.925 kb on - strand at 2726.997 kb on - strand at 2726.997 kb on - strand at 2726.997 kb on - strand at 2727.009 kb on + strand at 2727.018 kb on - strand at 2727.047 kb on + strand at 2727.047 kb on + strand at 2727.047 kb on + strand at 2727.048 kb on - strand at 2727.048 kb on - strand at 2727.048 kb on - strand at 2727.050 kb on - strand at 2727.100 kb on + strand at 2727.101 kb on - strand at 2727.101 kb on - strand at 2727.101 kb on - strand at 2727.101 kb on - strand at 2727.120 kb on + strand at 2727.121 kb on - strand at 2727.121 kb on - strand at 2727.157 kb on - strand at 2727.187 kb on + strand at 2727.188 kb on - strand at 2727.188 kb on - strand at 2727.207 kb on + strand at 2727.207 kb on + strand at 2727.207 kb on + strand at 2727.207 kb on + strand at 2727.207 kb on + strand at 2727.207 kb on + strand at 2727.208 kb on - strand at 2727.208 kb on - strand at 2727.208 kb on - strand at 2727.208 kb on - strand at 2727.208 kb on - strand at 2727.208 kb on - strand at 2727.208 kb on - strand at 2727.208 kb on - strand at 2727.208 kb on - strand at 2727.208 kb on - strand at 2727.219 kb on - strand at 2727.230 kb on - strand at 2727.380 kb on + strand, within Pf6N2E2_2302 at 2727.380 kb on + strand, within Pf6N2E2_2302 at 2727.380 kb on + strand, within Pf6N2E2_2302 at 2727.380 kb on + strand, within Pf6N2E2_2302 at 2727.380 kb on + strand, within Pf6N2E2_2302 at 2727.380 kb on + strand, within Pf6N2E2_2302 at 2727.381 kb on - strand, within Pf6N2E2_2302 at 2727.381 kb on - strand, within Pf6N2E2_2302 at 2727.381 kb on - strand, within Pf6N2E2_2302 at 2727.389 kb on + strand, within Pf6N2E2_2302 at 2727.490 kb on + strand, within Pf6N2E2_2302 at 2727.490 kb on + strand, within Pf6N2E2_2302 at 2727.490 kb on + strand, within Pf6N2E2_2302 at 2727.491 kb on - strand, within Pf6N2E2_2302 at 2727.491 kb on - strand, within Pf6N2E2_2302 at 2727.580 kb on + strand, within Pf6N2E2_2302 at 2727.708 kb on - strand, within Pf6N2E2_2302 at 2727.745 kb on + strand, within Pf6N2E2_2302 at 2727.745 kb on + strand, within Pf6N2E2_2302 at 2727.746 kb on - strand, within Pf6N2E2_2302 at 2727.836 kb on + strand, within Pf6N2E2_2302 at 2727.971 kb on + strand at 2727.972 kb on - strand at 2727.972 kb on - strand at 2727.975 kb on + strand at 2727.975 kb on + strand at 2727.975 kb on + strand at 2727.975 kb on + strand at 2727.976 kb on - strand at 2728.023 kb on + strand at 2728.052 kb on + strand at 2728.089 kb on - strand at 2728.176 kb on + strand at 2728.176 kb on + strand at 2728.375 kb on - strand, within Pf6N2E2_2303 at 2728.375 kb on - strand, within Pf6N2E2_2303 at 2728.375 kb on - strand, within Pf6N2E2_2303 at 2728.375 kb on - strand, within Pf6N2E2_2303 at 2728.375 kb on - strand, within Pf6N2E2_2303 at 2728.470 kb on + strand, within Pf6N2E2_2303 at 2728.470 kb on + strand, within Pf6N2E2_2303 at 2728.470 kb on + strand, within Pf6N2E2_2303 at 2728.471 kb on - strand, within Pf6N2E2_2303 at 2728.734 kb on + strand, within Pf6N2E2_2303 at 2728.734 kb on + strand, within Pf6N2E2_2303 at 2728.735 kb on - strand, within Pf6N2E2_2303 at 2728.735 kb on - strand, within Pf6N2E2_2303 at 2728.735 kb on - strand, within Pf6N2E2_2303 at 2728.735 kb on - strand, within Pf6N2E2_2303 at 2728.866 kb on - strand, within Pf6N2E2_2303 at 2728.878 kb on - strand, within Pf6N2E2_2303 at 2728.878 kb on - strand, within Pf6N2E2_2303
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 5 mM remove 2,726,277 + Pf6N2E2_2301 0.79 +0.0 2,726,382 + Pf6N2E2_2301 0.83 +1.2 2,726,382 + Pf6N2E2_2301 0.83 +1.9 2,726,382 + Pf6N2E2_2301 0.83 -0.5 2,726,382 + Pf6N2E2_2301 0.83 +0.7 2,726,382 + Pf6N2E2_2301 0.83 -0.2 2,726,382 + Pf6N2E2_2301 0.83 -0.8 2,726,382 + Pf6N2E2_2301 0.83 -0.0 2,726,383 - Pf6N2E2_2301 0.83 -1.1 2,726,383 - Pf6N2E2_2301 0.83 +0.2 2,726,383 - Pf6N2E2_2301 0.83 +0.2 2,726,383 - Pf6N2E2_2301 0.83 +0.9 2,726,383 - Pf6N2E2_2301 0.83 -2.1 2,726,383 - Pf6N2E2_2301 0.83 +0.1 2,726,383 - Pf6N2E2_2301 0.83 -0.3 2,726,383 - Pf6N2E2_2301 0.83 -2.5 2,726,412 + Pf6N2E2_2301 0.84 -0.7 2,726,412 + Pf6N2E2_2301 0.84 +1.2 2,726,412 + Pf6N2E2_2301 0.84 +0.2 2,726,430 + Pf6N2E2_2301 0.85 -1.6 2,726,430 + Pf6N2E2_2301 0.85 +1.2 2,726,430 + Pf6N2E2_2301 0.85 +0.1 2,726,430 + Pf6N2E2_2301 0.85 -0.5 2,726,430 + Pf6N2E2_2301 0.85 -1.0 2,726,431 - Pf6N2E2_2301 0.85 -1.0 2,726,476 - Pf6N2E2_2301 0.86 -0.1 2,726,562 - Pf6N2E2_2301 0.90 +0.4 2,726,771 + +0.3 2,726,790 + -0.7 2,726,791 - -0.2 2,726,791 - -0.3 2,726,791 - -0.9 2,726,791 - +1.3 2,726,817 + +0.8 2,726,817 + -0.7 2,726,817 + +0.7 2,726,817 + -0.2 2,726,817 + -0.7 2,726,817 + -0.5 2,726,817 + +0.6 2,726,818 - +1.3 2,726,818 - +0.7 2,726,818 - -0.8 2,726,818 - +1.6 2,726,818 - +0.5 2,726,818 - +0.3 2,726,818 - +0.4 2,726,818 - -0.3 2,726,818 - -0.2 2,726,844 + +0.5 2,726,844 + +1.3 2,726,844 + +0.3 2,726,861 + -1.6 2,726,861 + -0.7 2,726,925 - -0.5 2,726,925 - -0.7 2,726,997 - +0.7 2,726,997 - +1.3 2,726,997 - -0.7 2,727,009 + -0.3 2,727,018 - +1.1 2,727,047 + +1.3 2,727,047 + +0.3 2,727,047 + -1.6 2,727,048 - -0.3 2,727,048 - -0.5 2,727,048 - +0.5 2,727,050 - +0.9 2,727,100 + +0.7 2,727,101 - +0.5 2,727,101 - -0.5 2,727,101 - -1.2 2,727,101 - -1.9 2,727,120 + +0.3 2,727,121 - -0.9 2,727,121 - -1.1 2,727,157 - +0.3 2,727,187 + -1.1 2,727,188 - -0.1 2,727,188 - -0.7 2,727,207 + -0.3 2,727,207 + +0.1 2,727,207 + +0.3 2,727,207 + +0.3 2,727,207 + -0.3 2,727,207 + -0.1 2,727,208 - +1.3 2,727,208 - +0.8 2,727,208 - -0.7 2,727,208 - -1.5 2,727,208 - +0.7 2,727,208 - +0.7 2,727,208 - +0.3 2,727,208 - +1.5 2,727,208 - +0.3 2,727,208 - +0.8 2,727,219 - -0.0 2,727,230 - +0.3 2,727,380 + Pf6N2E2_2302 0.22 +0.1 2,727,380 + Pf6N2E2_2302 0.22 -0.7 2,727,380 + Pf6N2E2_2302 0.22 -0.1 2,727,380 + Pf6N2E2_2302 0.22 -1.1 2,727,380 + Pf6N2E2_2302 0.22 -0.3 2,727,380 + Pf6N2E2_2302 0.22 -0.1 2,727,381 - Pf6N2E2_2302 0.22 -0.0 2,727,381 - Pf6N2E2_2302 0.22 +0.6 2,727,381 - Pf6N2E2_2302 0.22 -0.7 2,727,389 + Pf6N2E2_2302 0.23 -0.3 2,727,490 + Pf6N2E2_2302 0.36 +2.8 2,727,490 + Pf6N2E2_2302 0.36 +0.7 2,727,490 + Pf6N2E2_2302 0.36 +0.0 2,727,491 - Pf6N2E2_2302 0.36 +1.3 2,727,491 - Pf6N2E2_2302 0.36 +0.5 2,727,580 + Pf6N2E2_2302 0.47 +1.3 2,727,708 - Pf6N2E2_2302 0.63 +1.3 2,727,745 + Pf6N2E2_2302 0.68 -0.1 2,727,745 + Pf6N2E2_2302 0.68 +0.7 2,727,746 - Pf6N2E2_2302 0.68 +0.3 2,727,836 + Pf6N2E2_2302 0.80 +0.5 2,727,971 + +1.3 2,727,972 - -1.6 2,727,972 - +1.3 2,727,975 + +0.6 2,727,975 + -1.7 2,727,975 + +2.5 2,727,975 + +0.4 2,727,976 - +1.1 2,728,023 + -1.1 2,728,052 + +1.6 2,728,089 - +2.0 2,728,176 + +0.3 2,728,176 + +0.4 2,728,375 - Pf6N2E2_2303 0.19 +1.2 2,728,375 - Pf6N2E2_2303 0.19 +0.0 2,728,375 - Pf6N2E2_2303 0.19 -0.8 2,728,375 - Pf6N2E2_2303 0.19 -1.1 2,728,375 - Pf6N2E2_2303 0.19 -0.6 2,728,470 + Pf6N2E2_2303 0.25 +1.2 2,728,470 + Pf6N2E2_2303 0.25 +0.9 2,728,470 + Pf6N2E2_2303 0.25 +0.6 2,728,471 - Pf6N2E2_2303 0.25 +1.2 2,728,734 + Pf6N2E2_2303 0.42 +1.2 2,728,734 + Pf6N2E2_2303 0.42 +0.8 2,728,735 - Pf6N2E2_2303 0.42 +0.4 2,728,735 - Pf6N2E2_2303 0.42 -0.3 2,728,735 - Pf6N2E2_2303 0.42 +1.2 2,728,735 - Pf6N2E2_2303 0.42 -1.0 2,728,866 - Pf6N2E2_2303 0.50 -0.1 2,728,878 - Pf6N2E2_2303 0.51 +0.8 2,728,878 - Pf6N2E2_2303 0.51 +0.5
Or see this region's nucleotide sequence