Experiment: Carbon source Sucrose 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1649 and Pf6N2E2_1650 overlap by 4 nucleotides Pf6N2E2_1650 and Pf6N2E2_1651 are separated by 84 nucleotides
Pf6N2E2_1649 - Maltose/maltodextrin transport ATP-binding protein MalK (EC 3.6.3.19), at 1,954,691 to 1,955,845
_1649
Pf6N2E2_1650 - Sucrose alpha-glucosidase (EC 3.2.1.48) (from data) , at 1,955,842 to 1,957,344
_1650
Pf6N2E2_1651 - Sucrose specific transcriptional regulator CscR, LacI family, at 1,957,429 to 1,958,412
_1651
Position (kb)
1955
1956
1957
1958 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1954.941 kb on - strand, within Pf6N2E2_1649 at 1954.949 kb on + strand, within Pf6N2E2_1649 at 1955.255 kb on + strand, within Pf6N2E2_1649 at 1955.255 kb on + strand, within Pf6N2E2_1649 at 1955.255 kb on + strand, within Pf6N2E2_1649 at 1955.256 kb on - strand, within Pf6N2E2_1649 at 1955.355 kb on - strand, within Pf6N2E2_1649 at 1955.355 kb on - strand, within Pf6N2E2_1649 at 1955.613 kb on - strand, within Pf6N2E2_1649 at 1955.841 kb on + strand at 1955.841 kb on + strand at 1955.841 kb on + strand at 1955.842 kb on - strand at 1955.954 kb on - strand at 1956.031 kb on + strand, within Pf6N2E2_1650 at 1956.032 kb on - strand, within Pf6N2E2_1650 at 1956.032 kb on - strand, within Pf6N2E2_1650 at 1956.032 kb on - strand, within Pf6N2E2_1650 at 1956.070 kb on + strand, within Pf6N2E2_1650 at 1956.070 kb on + strand, within Pf6N2E2_1650 at 1956.071 kb on - strand, within Pf6N2E2_1650 at 1956.209 kb on - strand, within Pf6N2E2_1650 at 1956.508 kb on + strand, within Pf6N2E2_1650 at 1956.508 kb on + strand, within Pf6N2E2_1650 at 1956.509 kb on - strand, within Pf6N2E2_1650 at 1956.563 kb on - strand, within Pf6N2E2_1650 at 1956.563 kb on - strand, within Pf6N2E2_1650 at 1956.592 kb on + strand, within Pf6N2E2_1650 at 1956.610 kb on + strand, within Pf6N2E2_1650 at 1956.611 kb on - strand, within Pf6N2E2_1650 at 1957.060 kb on + strand, within Pf6N2E2_1650 at 1957.061 kb on - strand, within Pf6N2E2_1650 at 1957.063 kb on + strand, within Pf6N2E2_1650 at 1957.176 kb on - strand, within Pf6N2E2_1650 at 1957.243 kb on + strand at 1957.275 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 5 mM remove 1,954,941 - Pf6N2E2_1649 0.22 -2.9 1,954,949 + Pf6N2E2_1649 0.22 -2.0 1,955,255 + Pf6N2E2_1649 0.49 -0.0 1,955,255 + Pf6N2E2_1649 0.49 -1.6 1,955,255 + Pf6N2E2_1649 0.49 -0.6 1,955,256 - Pf6N2E2_1649 0.49 -0.0 1,955,355 - Pf6N2E2_1649 0.57 -2.6 1,955,355 - Pf6N2E2_1649 0.57 -4.5 1,955,613 - Pf6N2E2_1649 0.80 -4.1 1,955,841 + +1.0 1,955,841 + -0.0 1,955,841 + -2.5 1,955,842 - -0.6 1,955,954 - -1.3 1,956,031 + Pf6N2E2_1650 0.13 -1.0 1,956,032 - Pf6N2E2_1650 0.13 -1.8 1,956,032 - Pf6N2E2_1650 0.13 -0.6 1,956,032 - Pf6N2E2_1650 0.13 -1.3 1,956,070 + Pf6N2E2_1650 0.15 -2.9 1,956,070 + Pf6N2E2_1650 0.15 -1.0 1,956,071 - Pf6N2E2_1650 0.15 -3.4 1,956,209 - Pf6N2E2_1650 0.24 -1.3 1,956,508 + Pf6N2E2_1650 0.44 -1.0 1,956,508 + Pf6N2E2_1650 0.44 -1.0 1,956,509 - Pf6N2E2_1650 0.44 -0.0 1,956,563 - Pf6N2E2_1650 0.48 -1.3 1,956,563 - Pf6N2E2_1650 0.48 +1.0 1,956,592 + Pf6N2E2_1650 0.50 -1.3 1,956,610 + Pf6N2E2_1650 0.51 -3.8 1,956,611 - Pf6N2E2_1650 0.51 -0.0 1,957,060 + Pf6N2E2_1650 0.81 -1.8 1,957,061 - Pf6N2E2_1650 0.81 -1.0 1,957,063 + Pf6N2E2_1650 0.81 -5.1 1,957,176 - Pf6N2E2_1650 0.89 -0.2 1,957,243 + -3.5 1,957,275 - -2.0
Or see this region's nucleotide sequence