Experiment: Carbon source Sucrose 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1151 and Pf6N2E2_1152 are separated by 94 nucleotides Pf6N2E2_1152 and Pf6N2E2_1153 are separated by 3 nucleotides Pf6N2E2_1153 and Pf6N2E2_1154 are separated by 77 nucleotides Pf6N2E2_1154 and Pf6N2E2_1155 are separated by 294 nucleotides
Pf6N2E2_1151 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 1,348,712 to 1,350,091
_1151
Pf6N2E2_1152 - hypothetical protein, at 1,350,186 to 1,350,920
_1152
Pf6N2E2_1153 - small molecule metabolism; degradation; fatty acid, at 1,350,924 to 1,351,739
_1153
Pf6N2E2_1154 - FIG00978082: hypothetical protein, at 1,351,817 to 1,352,404
_1154
Pf6N2E2_1155 - Cyn operon transcriptional activator, at 1,352,699 to 1,353,706
_1155
Position (kb)
1350
1351
1352 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1349.952 kb on + strand, within Pf6N2E2_1151 at 1349.952 kb on + strand, within Pf6N2E2_1151 at 1349.952 kb on + strand, within Pf6N2E2_1151 at 1350.060 kb on - strand at 1350.060 kb on - strand at 1350.060 kb on - strand at 1350.064 kb on - strand at 1350.064 kb on - strand at 1350.166 kb on + strand at 1350.191 kb on + strand at 1350.192 kb on - strand at 1350.230 kb on - strand at 1350.230 kb on - strand at 1350.406 kb on + strand, within Pf6N2E2_1152 at 1350.407 kb on - strand, within Pf6N2E2_1152 at 1350.667 kb on + strand, within Pf6N2E2_1152 at 1350.709 kb on + strand, within Pf6N2E2_1152 at 1350.709 kb on + strand, within Pf6N2E2_1152 at 1350.710 kb on - strand, within Pf6N2E2_1152 at 1350.871 kb on + strand at 1350.871 kb on + strand at 1350.871 kb on + strand at 1350.871 kb on + strand at 1350.872 kb on - strand at 1350.925 kb on + strand at 1350.925 kb on + strand at 1350.925 kb on + strand at 1350.925 kb on + strand at 1351.181 kb on - strand, within Pf6N2E2_1153 at 1351.270 kb on + strand, within Pf6N2E2_1153 at 1351.270 kb on + strand, within Pf6N2E2_1153 at 1351.271 kb on - strand, within Pf6N2E2_1153 at 1351.355 kb on - strand, within Pf6N2E2_1153 at 1351.447 kb on + strand, within Pf6N2E2_1153 at 1351.507 kb on + strand, within Pf6N2E2_1153 at 1351.514 kb on - strand, within Pf6N2E2_1153 at 1351.718 kb on + strand at 1351.743 kb on + strand at 1351.743 kb on + strand at 1351.744 kb on - strand at 1351.744 kb on - strand at 1351.744 kb on - strand at 1351.744 kb on - strand at 1351.744 kb on - strand at 1351.747 kb on + strand at 1351.748 kb on - strand at 1351.815 kb on - strand at 1351.904 kb on + strand, within Pf6N2E2_1154 at 1351.905 kb on - strand, within Pf6N2E2_1154 at 1351.957 kb on - strand, within Pf6N2E2_1154 at 1351.957 kb on - strand, within Pf6N2E2_1154 at 1351.957 kb on - strand, within Pf6N2E2_1154 at 1352.001 kb on + strand, within Pf6N2E2_1154 at 1352.001 kb on + strand, within Pf6N2E2_1154 at 1352.001 kb on + strand, within Pf6N2E2_1154 at 1352.002 kb on - strand, within Pf6N2E2_1154 at 1352.052 kb on + strand, within Pf6N2E2_1154 at 1352.053 kb on - strand, within Pf6N2E2_1154 at 1352.122 kb on - strand, within Pf6N2E2_1154 at 1352.122 kb on - strand, within Pf6N2E2_1154 at 1352.122 kb on - strand, within Pf6N2E2_1154 at 1352.293 kb on + strand, within Pf6N2E2_1154 at 1352.294 kb on - strand, within Pf6N2E2_1154 at 1352.495 kb on + strand at 1352.495 kb on + strand at 1352.495 kb on + strand at 1352.495 kb on + strand at 1352.496 kb on - strand at 1352.496 kb on - strand at 1352.496 kb on - strand at 1352.496 kb on - strand at 1352.564 kb on - strand at 1352.655 kb on + strand at 1352.655 kb on + strand at 1352.655 kb on + strand at 1352.655 kb on + strand at 1352.656 kb on - strand at 1352.656 kb on - strand at 1352.656 kb on - strand at 1352.672 kb on + strand at 1352.672 kb on + strand at 1352.672 kb on + strand at 1352.672 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 5 mM remove 1,349,952 + Pf6N2E2_1151 0.90 +0.9 1,349,952 + Pf6N2E2_1151 0.90 +0.9 1,349,952 + Pf6N2E2_1151 0.90 +0.9 1,350,060 - -1.1 1,350,060 - -1.7 1,350,060 - -1.7 1,350,064 - -0.1 1,350,064 - -0.1 1,350,166 + +0.9 1,350,191 + +0.9 1,350,192 - +0.3 1,350,230 - -0.7 1,350,230 - -1.5 1,350,406 + Pf6N2E2_1152 0.30 +0.3 1,350,407 - Pf6N2E2_1152 0.30 -0.1 1,350,667 + Pf6N2E2_1152 0.65 +0.1 1,350,709 + Pf6N2E2_1152 0.71 -1.5 1,350,709 + Pf6N2E2_1152 0.71 -0.1 1,350,710 - Pf6N2E2_1152 0.71 -0.1 1,350,871 + +0.9 1,350,871 + +0.2 1,350,871 + +0.9 1,350,871 + -1.6 1,350,872 - -0.1 1,350,925 + -2.0 1,350,925 + +0.9 1,350,925 + +0.4 1,350,925 + -1.3 1,351,181 - Pf6N2E2_1153 0.31 +0.9 1,351,270 + Pf6N2E2_1153 0.42 +0.9 1,351,270 + Pf6N2E2_1153 0.42 -0.7 1,351,271 - Pf6N2E2_1153 0.43 +1.7 1,351,355 - Pf6N2E2_1153 0.53 -2.0 1,351,447 + Pf6N2E2_1153 0.64 -2.1 1,351,507 + Pf6N2E2_1153 0.71 -1.4 1,351,514 - Pf6N2E2_1153 0.72 -1.5 1,351,718 + +1.3 1,351,743 + +0.9 1,351,743 + +0.3 1,351,744 - -0.4 1,351,744 - +0.3 1,351,744 - +0.9 1,351,744 - -1.7 1,351,744 - -1.0 1,351,747 + +0.9 1,351,748 - -1.4 1,351,815 - -0.5 1,351,904 + Pf6N2E2_1154 0.15 -0.7 1,351,905 - Pf6N2E2_1154 0.15 +0.9 1,351,957 - Pf6N2E2_1154 0.24 +0.9 1,351,957 - Pf6N2E2_1154 0.24 -2.0 1,351,957 - Pf6N2E2_1154 0.24 -0.1 1,352,001 + Pf6N2E2_1154 0.31 -0.4 1,352,001 + Pf6N2E2_1154 0.31 -0.1 1,352,001 + Pf6N2E2_1154 0.31 -1.1 1,352,002 - Pf6N2E2_1154 0.31 +0.9 1,352,052 + Pf6N2E2_1154 0.40 +0.9 1,352,053 - Pf6N2E2_1154 0.40 -1.6 1,352,122 - Pf6N2E2_1154 0.52 +0.9 1,352,122 - Pf6N2E2_1154 0.52 -3.0 1,352,122 - Pf6N2E2_1154 0.52 +0.9 1,352,293 + Pf6N2E2_1154 0.81 -0.1 1,352,294 - Pf6N2E2_1154 0.81 +0.9 1,352,495 + +0.9 1,352,495 + +0.1 1,352,495 + -0.1 1,352,495 + -0.7 1,352,496 - -0.4 1,352,496 - +0.0 1,352,496 - +0.2 1,352,496 - +0.9 1,352,564 - +0.9 1,352,655 + -0.5 1,352,655 + -0.7 1,352,655 + -0.4 1,352,655 + -0.9 1,352,656 - +1.4 1,352,656 - +1.7 1,352,656 - -0.1 1,352,672 + +0.7 1,352,672 + +0.9 1,352,672 + +0.9 1,352,672 + +0.9
Or see this region's nucleotide sequence