Experiment: Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01885 and BWI76_RS01890 are separated by 90 nucleotides BWI76_RS01890 and BWI76_RS01895 are separated by 192 nucleotides BWI76_RS01895 and BWI76_RS01900 are separated by 107 nucleotides
BWI76_RS01885: BWI76_RS01885 - CsbD family protein, at 385,233 to 385,445
_RS01885
BWI76_RS01890: BWI76_RS01890 - transcriptional regulator Zur, at 385,536 to 386,051
_RS01890
BWI76_RS01895: BWI76_RS01895 - cupin, at 386,244 to 386,732
_RS01895
BWI76_RS01900: BWI76_RS01900 - tRNA-dihydrouridine synthase A, at 386,840 to 387,835
_RS01900
Position (kb)
386
387 Strain fitness (log2 ratio)
-2
-1
0
1 at 385.328 kb on + strand, within BWI76_RS01885 at 385.599 kb on - strand, within BWI76_RS01890 at 385.746 kb on + strand, within BWI76_RS01890 at 385.913 kb on + strand, within BWI76_RS01890 at 385.921 kb on - strand, within BWI76_RS01890 at 385.921 kb on - strand, within BWI76_RS01890 at 385.937 kb on + strand, within BWI76_RS01890 at 386.040 kb on + strand at 386.186 kb on - strand at 386.208 kb on + strand at 386.209 kb on - strand at 386.213 kb on + strand at 386.295 kb on - strand, within BWI76_RS01895 at 386.314 kb on + strand, within BWI76_RS01895 at 386.322 kb on - strand, within BWI76_RS01895 at 386.475 kb on - strand, within BWI76_RS01895 at 387.444 kb on - strand, within BWI76_RS01900 at 387.475 kb on - strand, within BWI76_RS01900 at 387.505 kb on + strand, within BWI76_RS01900 at 387.657 kb on + strand, within BWI76_RS01900 at 387.675 kb on + strand, within BWI76_RS01900
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB remove 385,328 + BWI76_RS01885 0.45 -1.2 385,599 - BWI76_RS01890 0.12 +0.6 385,746 + BWI76_RS01890 0.41 -0.7 385,913 + BWI76_RS01890 0.73 +1.2 385,921 - BWI76_RS01890 0.75 -0.7 385,921 - BWI76_RS01890 0.75 -1.8 385,937 + BWI76_RS01890 0.78 -1.2 386,040 + -0.9 386,186 - -0.1 386,208 + -0.6 386,209 - -0.7 386,213 + -0.9 386,295 - BWI76_RS01895 0.10 +0.0 386,314 + BWI76_RS01895 0.14 -0.3 386,322 - BWI76_RS01895 0.16 +1.6 386,475 - BWI76_RS01895 0.47 +0.6 387,444 - BWI76_RS01900 0.61 -0.6 387,475 - BWI76_RS01900 0.64 +0.7 387,505 + BWI76_RS01900 0.67 -0.1 387,657 + BWI76_RS01900 0.82 -0.2 387,675 + BWI76_RS01900 0.84 +0.3
Or see this region's nucleotide sequence