Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20615 and BWI76_RS20620 overlap by 4 nucleotides BWI76_RS20620 and BWI76_RS20625 overlap by 8 nucleotides BWI76_RS20625 and BWI76_RS20630 are separated by 74 nucleotides
BWI76_RS20615: BWI76_RS20615 - sodium symporter, at 4,313,756 to 4,314,754
_RS20615
BWI76_RS20620: BWI76_RS20620 - negative regulator, at 4,314,751 to 4,314,969
_RS20620
BWI76_RS20625: BWI76_RS20625 - DNA ligase (NAD(+)) LigA, at 4,314,962 to 4,316,986
_RS20625
BWI76_RS20630: BWI76_RS20630 - cell division protein ZipA, at 4,317,061 to 4,318,056
_RS20630
Position (kb)
4314
4315
4316
4317 Strain fitness (log2 ratio)
-2
-1
0
1 at 4314.152 kb on + strand, within BWI76_RS20615 at 4314.227 kb on + strand, within BWI76_RS20615 at 4314.234 kb on + strand, within BWI76_RS20615 at 4314.242 kb on - strand, within BWI76_RS20615 at 4314.604 kb on + strand, within BWI76_RS20615 at 4314.673 kb on - strand at 4314.769 kb on - strand at 4314.769 kb on - strand at 4314.784 kb on + strand, within BWI76_RS20620 at 4317.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB remove 4,314,152 + BWI76_RS20615 0.40 -0.1 4,314,227 + BWI76_RS20615 0.47 +0.1 4,314,234 + BWI76_RS20615 0.48 -0.0 4,314,242 - BWI76_RS20615 0.49 -0.4 4,314,604 + BWI76_RS20615 0.85 +0.3 4,314,673 - -0.2 4,314,769 - -1.8 4,314,769 - -0.8 4,314,784 + BWI76_RS20620 0.15 +0.3 4,317,073 - +0.6
Or see this region's nucleotide sequence