Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04570 and BWI76_RS04575 are separated by 187 nucleotides BWI76_RS04575 and BWI76_RS04580 are separated by 214 nucleotides BWI76_RS04580 and BWI76_RS04585 are separated by 73 nucleotides
BWI76_RS04570: BWI76_RS04570 - RNA polymerase-associated protein RapA, at 946,783 to 949,689
_RS04570
BWI76_RS04575: BWI76_RS04575 - DNA polymerase II, at 949,877 to 952,234
_RS04575
BWI76_RS04580: BWI76_RS04580 - L-ribulose-5-phosphate 4-epimerase, at 952,449 to 953,144
_RS04580
BWI76_RS04585: BWI76_RS04585 - L-arabinose isomerase, at 953,218 to 954,720
_RS04585
Position (kb) 949
950
951
952
953 Strain fitness (log2 ratio) -2
-1
0
1 at 948.965 kb on - strand, within BWI76_RS04570 at 948.970 kb on + strand, within BWI76_RS04570 at 949.017 kb on + strand, within BWI76_RS04570 at 949.025 kb on - strand, within BWI76_RS04570 at 949.520 kb on + strand at 949.766 kb on - strand at 949.800 kb on - strand at 949.939 kb on - strand at 949.988 kb on - strand at 950.031 kb on + strand at 950.082 kb on + strand at 950.143 kb on + strand, within BWI76_RS04575 at 950.162 kb on + strand, within BWI76_RS04575 at 950.170 kb on - strand, within BWI76_RS04575 at 950.210 kb on - strand, within BWI76_RS04575 at 950.339 kb on - strand, within BWI76_RS04575 at 950.463 kb on + strand, within BWI76_RS04575 at 950.463 kb on - strand, within BWI76_RS04575 at 950.793 kb on - strand, within BWI76_RS04575 at 950.813 kb on - strand, within BWI76_RS04575 at 950.872 kb on - strand, within BWI76_RS04575 at 950.889 kb on + strand, within BWI76_RS04575 at 950.956 kb on + strand, within BWI76_RS04575 at 950.986 kb on - strand, within BWI76_RS04575 at 951.011 kb on - strand, within BWI76_RS04575 at 951.030 kb on + strand, within BWI76_RS04575 at 951.041 kb on + strand, within BWI76_RS04575 at 951.043 kb on - strand, within BWI76_RS04575 at 951.051 kb on + strand, within BWI76_RS04575 at 951.416 kb on + strand, within BWI76_RS04575 at 951.430 kb on - strand, within BWI76_RS04575 at 951.556 kb on + strand, within BWI76_RS04575 at 951.723 kb on - strand, within BWI76_RS04575 at 951.734 kb on - strand, within BWI76_RS04575 at 952.102 kb on - strand at 952.274 kb on - strand at 952.441 kb on - strand at 952.643 kb on + strand, within BWI76_RS04580 at 952.865 kb on - strand, within BWI76_RS04580 at 952.865 kb on - strand, within BWI76_RS04580 at 952.915 kb on - strand, within BWI76_RS04580 at 952.935 kb on - strand, within BWI76_RS04580 at 952.947 kb on - strand, within BWI76_RS04580 at 953.039 kb on + strand, within BWI76_RS04580 at 953.089 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB remove 948,965 - BWI76_RS04570 0.75 +0.2 948,970 + BWI76_RS04570 0.75 -0.9 949,017 + BWI76_RS04570 0.77 -0.1 949,025 - BWI76_RS04570 0.77 -0.0 949,520 + -0.9 949,766 - -0.5 949,800 - -0.8 949,939 - -0.4 949,988 - +0.1 950,031 + +0.3 950,082 + -0.5 950,143 + BWI76_RS04575 0.11 -0.5 950,162 + BWI76_RS04575 0.12 -0.5 950,170 - BWI76_RS04575 0.12 -0.3 950,210 - BWI76_RS04575 0.14 +0.1 950,339 - BWI76_RS04575 0.20 -1.9 950,463 + BWI76_RS04575 0.25 +0.0 950,463 - BWI76_RS04575 0.25 +0.2 950,793 - BWI76_RS04575 0.39 -1.3 950,813 - BWI76_RS04575 0.40 -0.4 950,872 - BWI76_RS04575 0.42 +0.4 950,889 + BWI76_RS04575 0.43 +0.0 950,956 + BWI76_RS04575 0.46 -1.0 950,986 - BWI76_RS04575 0.47 +0.7 951,011 - BWI76_RS04575 0.48 +1.6 951,030 + BWI76_RS04575 0.49 +0.2 951,041 + BWI76_RS04575 0.49 +0.7 951,043 - BWI76_RS04575 0.49 -1.4 951,051 + BWI76_RS04575 0.50 -0.2 951,416 + BWI76_RS04575 0.65 -1.3 951,430 - BWI76_RS04575 0.66 -0.0 951,556 + BWI76_RS04575 0.71 +0.3 951,723 - BWI76_RS04575 0.78 -0.5 951,734 - BWI76_RS04575 0.79 -0.7 952,102 - -0.2 952,274 - +0.1 952,441 - -1.0 952,643 + BWI76_RS04580 0.28 +0.1 952,865 - BWI76_RS04580 0.60 -0.8 952,865 - BWI76_RS04580 0.60 -1.2 952,915 - BWI76_RS04580 0.67 -0.5 952,935 - BWI76_RS04580 0.70 -0.9 952,947 - BWI76_RS04580 0.72 -0.0 953,039 + BWI76_RS04580 0.85 -0.4 953,089 + -0.6
Or see this region's nucleotide sequence