Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.8X; growth supplemented with 0.4X LB 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt BWI76_RS22320 and BWI76_RS22325 are separated by 17 nucleotides BWI76_RS22325 and BWI76_RS22330 are separated by 2 nucleotides BWI76_RS22330 and BWI76_RS22335 overlap by 4 nucleotides BWI76_RS22335 and BWI76_RS22340 are separated by 82 nucleotides  
        
        BWI76_RS22320: BWI76_RS22320 - hydrogenase 3 large subunit, at 4,652,151 to 4,653,860 
         
        _RS22320 
         
        
        BWI76_RS22325: BWI76_RS22325 - hydrogenase 3 membrane subunit, at 4,653,878 to 4,654,801 
         
        _RS22325 
         
        
        BWI76_RS22330: BWI76_RS22330 - formate hydrogenlyase subunit 3, at 4,654,804 to 4,656,630 
         
        _RS22330 
         
        
        BWI76_RS22335: BWI76_RS22335 - 4Fe-4S ferredoxin, at 4,656,627 to 4,657,235 
         
        _RS22335 
         
        
        BWI76_RS22340: BWI76_RS22340 - formate hydrogenlyase regulatory protein HycA, at 4,657,318 to 4,657,767 
         
        _RS22340 
         Position (kb)  
4654 
 
4655 
 
4656 
 
4657 Strain fitness (log2 ratio)  
-2 
 
-1 
 
0 
 
1 at 4653.956 kb on - strand at 4654.110 kb on + strand, within BWI76_RS22325 at 4654.128 kb on - strand, within BWI76_RS22325 at 4654.312 kb on - strand, within BWI76_RS22325 at 4654.328 kb on + strand, within BWI76_RS22325 at 4654.367 kb on - strand, within BWI76_RS22325 at 4654.576 kb on + strand, within BWI76_RS22325 at 4654.862 kb on + strand at 4655.163 kb on - strand, within BWI76_RS22330 at 4655.294 kb on - strand, within BWI76_RS22330 at 4655.321 kb on - strand, within BWI76_RS22330 at 4655.682 kb on - strand, within BWI76_RS22330 at 4655.909 kb on + strand, within BWI76_RS22330 at 4656.349 kb on - strand, within BWI76_RS22330 at 4656.400 kb on + strand, within BWI76_RS22330 at 4656.438 kb on + strand, within BWI76_RS22330 at 4656.474 kb on - strand at 4656.770 kb on - strand, within BWI76_RS22335 at 4656.836 kb on + strand, within BWI76_RS22335 at 4656.849 kb on - strand, within BWI76_RS22335 at 4656.883 kb on + strand, within BWI76_RS22335 at 4656.921 kb on - strand, within BWI76_RS22335 at 4657.292 kb on - strand at 4657.609 kb on - strand, within BWI76_RS22340  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.8X; growth supplemented with 0.4X LB             remove   4,653,956   -        -0.0   4,654,110   +    BWI76_RS22325   0.25  -0.4   4,654,128   -    BWI76_RS22325   0.27  -0.3   4,654,312   -    BWI76_RS22325   0.47  -1.5   4,654,328   +    BWI76_RS22325   0.49  -1.1   4,654,367   -    BWI76_RS22325   0.53  -0.6   4,654,576   +    BWI76_RS22325   0.76  -1.0   4,654,862   +        +0.3   4,655,163   -    BWI76_RS22330   0.20  -0.7   4,655,294   -    BWI76_RS22330   0.27  -0.3   4,655,321   -    BWI76_RS22330   0.28  -0.1   4,655,682   -    BWI76_RS22330   0.48  -1.3   4,655,909   +    BWI76_RS22330   0.60  -0.5   4,656,349   -    BWI76_RS22330   0.85  +0.6   4,656,400   +    BWI76_RS22330   0.87  -1.5   4,656,438   +    BWI76_RS22330   0.89  -0.3   4,656,474   -        +0.1   4,656,770   -    BWI76_RS22335   0.23  -1.9   4,656,836   +    BWI76_RS22335   0.34  -0.7   4,656,849   -    BWI76_RS22335   0.36  -0.6   4,656,883   +    BWI76_RS22335   0.42  +1.2   4,656,921   -    BWI76_RS22335   0.48  -0.4   4,657,292   -        -1.1   4,657,609   -    BWI76_RS22340   0.65  -0.3 
 
Or see this region's nucleotide sequence