Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.8X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14535 and BWI76_RS14540 overlap by 4 nucleotides BWI76_RS14540 and BWI76_RS14545 overlap by 4 nucleotides BWI76_RS14545 and BWI76_RS14550 are separated by 3 nucleotides
BWI76_RS14535: BWI76_RS14535 - polyketide synthase, at 3,042,203 to 3,051,679
_RS14535
BWI76_RS14540: BWI76_RS14540 - yersiniabactin biosynthetic protein YbtU, at 3,051,676 to 3,052,773
_RS14540
BWI76_RS14545: BWI76_RS14545 - thioesterase, at 3,052,770 to 3,053,573
_RS14545
BWI76_RS14550: BWI76_RS14550 - 2,3-dihydroxybenzoate-AMP ligase, at 3,053,577 to 3,055,154
_RS14550
Position (kb)
3051
3052
3053 Strain fitness (log2 ratio)
-1
0
1 at 3050.710 kb on + strand, within BWI76_RS14535 at 3050.788 kb on + strand at 3050.838 kb on + strand at 3050.875 kb on - strand at 3051.608 kb on + strand at 3051.616 kb on - strand at 3051.749 kb on + strand at 3051.848 kb on + strand, within BWI76_RS14540 at 3052.055 kb on + strand, within BWI76_RS14540 at 3052.411 kb on + strand, within BWI76_RS14540 at 3052.830 kb on + strand at 3052.914 kb on + strand, within BWI76_RS14545 at 3052.948 kb on + strand, within BWI76_RS14545 at 3053.344 kb on + strand, within BWI76_RS14545 at 3053.384 kb on - strand, within BWI76_RS14545 at 3053.562 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.8X; growth supplemented with 0.4X LB remove 3,050,710 + BWI76_RS14535 0.90 -0.4 3,050,788 + -0.2 3,050,838 + -1.0 3,050,875 - +0.5 3,051,608 + -0.3 3,051,616 - +0.8 3,051,749 + -0.4 3,051,848 + BWI76_RS14540 0.16 +0.7 3,052,055 + BWI76_RS14540 0.35 +0.2 3,052,411 + BWI76_RS14540 0.67 +1.5 3,052,830 + +0.0 3,052,914 + BWI76_RS14545 0.18 -0.5 3,052,948 + BWI76_RS14545 0.22 -0.2 3,053,344 + BWI76_RS14545 0.71 -0.4 3,053,384 - BWI76_RS14545 0.76 +0.0 3,053,562 + -0.1
Or see this region's nucleotide sequence