Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.8X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02445 and BWI76_RS02450 are separated by 10 nucleotides BWI76_RS02450 and BWI76_RS02455 overlap by 8 nucleotides BWI76_RS02455 and BWI76_RS02460 are separated by 275 nucleotides
BWI76_RS02445: BWI76_RS02445 - fumarate reductase subunit C, at 500,534 to 500,929
_RS02445
BWI76_RS02450: BWI76_RS02450 - succinate dehydrogenase/fumarate reductase iron-sulfur subunit, at 500,940 to 501,674
_RS02450
BWI76_RS02455: BWI76_RS02455 - fumarate reductase flavoprotein subunit, at 501,667 to 503,457
_RS02455
BWI76_RS02460: BWI76_RS02460 - elongation factor P lysine(34) lysyltransferase, at 503,733 to 504,710
_RS02460
Position (kb) 501
502
503
504 Strain fitness (log2 ratio) -1
0
1 at 500.762 kb on + strand, within BWI76_RS02445 at 500.848 kb on + strand, within BWI76_RS02445 at 500.850 kb on + strand, within BWI76_RS02445 at 500.911 kb on - strand at 500.948 kb on + strand at 500.956 kb on - strand at 500.994 kb on - strand at 501.287 kb on + strand, within BWI76_RS02450 at 501.420 kb on - strand, within BWI76_RS02450 at 501.475 kb on - strand, within BWI76_RS02450 at 501.505 kb on + strand, within BWI76_RS02450 at 501.552 kb on - strand, within BWI76_RS02450 at 501.601 kb on + strand at 501.601 kb on + strand at 501.688 kb on + strand at 501.874 kb on - strand, within BWI76_RS02455 at 501.941 kb on + strand, within BWI76_RS02455 at 501.941 kb on + strand, within BWI76_RS02455 at 501.949 kb on - strand, within BWI76_RS02455 at 501.949 kb on - strand, within BWI76_RS02455 at 501.964 kb on - strand, within BWI76_RS02455 at 501.974 kb on - strand, within BWI76_RS02455 at 502.086 kb on + strand, within BWI76_RS02455 at 502.122 kb on - strand, within BWI76_RS02455 at 502.305 kb on - strand, within BWI76_RS02455 at 502.381 kb on + strand, within BWI76_RS02455 at 502.439 kb on + strand, within BWI76_RS02455 at 502.483 kb on - strand, within BWI76_RS02455 at 502.493 kb on - strand, within BWI76_RS02455 at 502.529 kb on - strand, within BWI76_RS02455 at 502.552 kb on + strand, within BWI76_RS02455 at 502.553 kb on + strand, within BWI76_RS02455 at 502.577 kb on + strand, within BWI76_RS02455 at 502.607 kb on - strand, within BWI76_RS02455 at 503.038 kb on - strand, within BWI76_RS02455 at 503.087 kb on + strand, within BWI76_RS02455 at 503.144 kb on + strand, within BWI76_RS02455 at 503.153 kb on + strand, within BWI76_RS02455 at 503.313 kb on - strand at 503.319 kb on + strand at 503.409 kb on - strand at 503.414 kb on - strand at 503.513 kb on + strand at 503.658 kb on - strand at 503.658 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.8X; growth supplemented with 0.4X LB remove 500,762 + BWI76_RS02445 0.58 +0.2 500,848 + BWI76_RS02445 0.79 +0.1 500,850 + BWI76_RS02445 0.80 -0.6 500,911 - -0.3 500,948 + +0.4 500,956 - -0.7 500,994 - -0.4 501,287 + BWI76_RS02450 0.47 -0.9 501,420 - BWI76_RS02450 0.65 -0.4 501,475 - BWI76_RS02450 0.73 -0.3 501,505 + BWI76_RS02450 0.77 -0.6 501,552 - BWI76_RS02450 0.83 -0.6 501,601 + +0.3 501,601 + -0.9 501,688 + +0.2 501,874 - BWI76_RS02455 0.12 -0.2 501,941 + BWI76_RS02455 0.15 -1.5 501,941 + BWI76_RS02455 0.15 +0.1 501,949 - BWI76_RS02455 0.16 -0.4 501,949 - BWI76_RS02455 0.16 +0.1 501,964 - BWI76_RS02455 0.17 -0.1 501,974 - BWI76_RS02455 0.17 -0.4 502,086 + BWI76_RS02455 0.23 +0.0 502,122 - BWI76_RS02455 0.25 +0.8 502,305 - BWI76_RS02455 0.36 -0.5 502,381 + BWI76_RS02455 0.40 -0.3 502,439 + BWI76_RS02455 0.43 -1.5 502,483 - BWI76_RS02455 0.46 -1.3 502,493 - BWI76_RS02455 0.46 +0.1 502,529 - BWI76_RS02455 0.48 -0.4 502,552 + BWI76_RS02455 0.49 -0.2 502,553 + BWI76_RS02455 0.49 -0.5 502,577 + BWI76_RS02455 0.51 -0.8 502,607 - BWI76_RS02455 0.52 +0.0 503,038 - BWI76_RS02455 0.77 +0.4 503,087 + BWI76_RS02455 0.79 +0.5 503,144 + BWI76_RS02455 0.82 -0.3 503,153 + BWI76_RS02455 0.83 -0.4 503,313 - -0.6 503,319 + +1.2 503,409 - -0.4 503,414 - -0.4 503,513 + -0.2 503,658 - -0.4 503,658 - -0.8
Or see this region's nucleotide sequence