Experiment: Vogels_fungal_media; pH 5.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01275 and BWI76_RS01280 are separated by 232 nucleotides BWI76_RS01280 and BWI76_RS01285 are separated by 165 nucleotides BWI76_RS01285 and BWI76_RS01290 are separated by 3 nucleotides
BWI76_RS01275: BWI76_RS01275 - LysR family transcriptional regulator, at 260,795 to 261,748
_RS01275
BWI76_RS01280: BWI76_RS01280 - 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase, at 261,981 to 264,242
_RS01280
BWI76_RS01285: BWI76_RS01285 - amino acid ABC transporter substrate-binding protein, at 264,408 to 265,097
_RS01285
BWI76_RS01290: BWI76_RS01290 - putative dienelactone hydrolase, at 265,101 to 265,913
_RS01290
Position (kb) 261
262
263
264
265 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 260.996 kb on + strand, within BWI76_RS01275 at 261.005 kb on - strand, within BWI76_RS01275 at 261.109 kb on - strand, within BWI76_RS01275 at 261.114 kb on - strand, within BWI76_RS01275 at 261.435 kb on - strand, within BWI76_RS01275 at 261.625 kb on + strand, within BWI76_RS01275 at 261.633 kb on - strand, within BWI76_RS01275 at 261.663 kb on + strand at 261.699 kb on + strand at 261.904 kb on - strand at 262.023 kb on - strand at 262.089 kb on - strand at 262.183 kb on + strand at 262.201 kb on + strand at 262.226 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.504 kb on - strand, within BWI76_RS01280 at 262.517 kb on - strand, within BWI76_RS01280 at 262.692 kb on - strand, within BWI76_RS01280 at 262.692 kb on - strand, within BWI76_RS01280 at 262.901 kb on + strand, within BWI76_RS01280 at 262.909 kb on - strand, within BWI76_RS01280 at 263.014 kb on + strand, within BWI76_RS01280 at 263.072 kb on - strand, within BWI76_RS01280 at 263.247 kb on - strand, within BWI76_RS01280 at 263.290 kb on + strand, within BWI76_RS01280 at 263.298 kb on - strand, within BWI76_RS01280 at 263.345 kb on + strand, within BWI76_RS01280 at 263.406 kb on + strand, within BWI76_RS01280 at 263.459 kb on - strand, within BWI76_RS01280 at 263.513 kb on - strand, within BWI76_RS01280 at 263.518 kb on - strand, within BWI76_RS01280 at 263.525 kb on - strand, within BWI76_RS01280 at 263.535 kb on - strand, within BWI76_RS01280 at 263.901 kb on + strand, within BWI76_RS01280 at 263.906 kb on + strand, within BWI76_RS01280 at 264.050 kb on + strand at 264.087 kb on - strand at 264.104 kb on + strand at 264.142 kb on + strand at 264.217 kb on - strand at 264.217 kb on - strand at 264.217 kb on - strand at 264.319 kb on - strand at 264.367 kb on - strand at 264.448 kb on + strand at 264.505 kb on + strand, within BWI76_RS01285 at 264.568 kb on - strand, within BWI76_RS01285 at 264.662 kb on - strand, within BWI76_RS01285 at 264.761 kb on - strand, within BWI76_RS01285 at 264.958 kb on + strand, within BWI76_RS01285 at 264.992 kb on + strand, within BWI76_RS01285 at 265.007 kb on - strand, within BWI76_RS01285 at 265.060 kb on - strand at 265.099 kb on - strand at 265.132 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media; pH 5.5 remove 260,996 + BWI76_RS01275 0.21 -2.4 261,005 - BWI76_RS01275 0.22 -3.9 261,109 - BWI76_RS01275 0.33 -6.5 261,114 - BWI76_RS01275 0.33 -3.1 261,435 - BWI76_RS01275 0.67 -3.2 261,625 + BWI76_RS01275 0.87 -4.0 261,633 - BWI76_RS01275 0.88 -4.1 261,663 + -4.8 261,699 + -3.0 261,904 - -3.9 262,023 - -2.9 262,089 - -2.7 262,183 + -3.3 262,201 + -4.9 262,226 - BWI76_RS01280 0.11 -4.1 262,504 - BWI76_RS01280 0.23 -1.0 262,504 - BWI76_RS01280 0.23 -2.7 262,504 - BWI76_RS01280 0.23 -0.4 262,504 - BWI76_RS01280 0.23 -3.4 262,504 - BWI76_RS01280 0.23 -3.0 262,504 - BWI76_RS01280 0.23 -2.3 262,517 - BWI76_RS01280 0.24 -2.8 262,692 - BWI76_RS01280 0.31 -3.0 262,692 - BWI76_RS01280 0.31 -3.2 262,901 + BWI76_RS01280 0.41 -3.0 262,909 - BWI76_RS01280 0.41 -5.3 263,014 + BWI76_RS01280 0.46 -4.2 263,072 - BWI76_RS01280 0.48 -4.1 263,247 - BWI76_RS01280 0.56 -3.6 263,290 + BWI76_RS01280 0.58 -4.2 263,298 - BWI76_RS01280 0.58 -1.2 263,345 + BWI76_RS01280 0.60 -3.2 263,406 + BWI76_RS01280 0.63 -3.3 263,459 - BWI76_RS01280 0.65 -1.8 263,513 - BWI76_RS01280 0.68 -3.7 263,518 - BWI76_RS01280 0.68 -3.5 263,525 - BWI76_RS01280 0.68 +1.8 263,535 - BWI76_RS01280 0.69 -2.8 263,901 + BWI76_RS01280 0.85 -3.1 263,906 + BWI76_RS01280 0.85 -2.3 264,050 + -3.6 264,087 - -3.9 264,104 + -4.0 264,142 + -5.3 264,217 - -3.4 264,217 - -3.1 264,217 - -1.0 264,319 - -0.5 264,367 - -1.0 264,448 + -1.6 264,505 + BWI76_RS01285 0.14 +0.9 264,568 - BWI76_RS01285 0.23 -0.3 264,662 - BWI76_RS01285 0.37 +0.6 264,761 - BWI76_RS01285 0.51 +1.2 264,958 + BWI76_RS01285 0.80 +0.5 264,992 + BWI76_RS01285 0.85 +0.1 265,007 - BWI76_RS01285 0.87 +0.1 265,060 - +1.7 265,099 - -4.2 265,132 - +0.0
Or see this region's nucleotide sequence