Experiment: Vogels_fungal_media; pH 5.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01185 and BWI76_RS01190 are separated by 80 nucleotides BWI76_RS01190 and BWI76_RS01195 are separated by 138 nucleotides BWI76_RS01195 and BWI76_RS01200 are separated by 25 nucleotides
BWI76_RS01185: BWI76_RS01185 - adenylate cyclase, at 242,042 to 244,591
_RS01185
BWI76_RS01190: BWI76_RS01190 - iron donor protein CyaY, at 244,672 to 244,992
_RS01190
BWI76_RS01195: BWI76_RS01195 - membrane protein, at 245,131 to 245,334
_RS01195
BWI76_RS01200: BWI76_RS01200 - diaminopimelate epimerase, at 245,360 to 246,184
_RS01200
Position (kb) 244
245 Strain fitness (log2 ratio) -2
-1
0
1
2 at 243.956 kb on - strand, within BWI76_RS01185 at 243.956 kb on - strand, within BWI76_RS01185 at 244.498 kb on + strand at 244.555 kb on - strand at 244.608 kb on + strand at 244.876 kb on - strand, within BWI76_RS01190 at 244.920 kb on - strand, within BWI76_RS01190 at 245.186 kb on + strand, within BWI76_RS01195 at 245.194 kb on - strand, within BWI76_RS01195 at 245.337 kb on + strand at 245.809 kb on - strand, within BWI76_RS01200 at 245.809 kb on - strand, within BWI76_RS01200 at 245.838 kb on + strand, within BWI76_RS01200 at 245.838 kb on + strand, within BWI76_RS01200
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media; pH 5.5 remove 243,956 - BWI76_RS01185 0.75 -1.2 243,956 - BWI76_RS01185 0.75 +0.8 244,498 + -1.2 244,555 - +0.8 244,608 + -0.7 244,876 - BWI76_RS01190 0.64 -0.7 244,920 - BWI76_RS01190 0.77 -0.1 245,186 + BWI76_RS01195 0.27 -2.4 245,194 - BWI76_RS01195 0.31 -2.6 245,337 + -1.2 245,809 - BWI76_RS01200 0.54 -0.8 245,809 - BWI76_RS01200 0.54 -2.2 245,838 + BWI76_RS01200 0.58 -0.5 245,838 + BWI76_RS01200 0.58 +1.8
Or see this region's nucleotide sequence