Experiment: Vogels_fungal_media; pH 5.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08545 and BWI76_RS08550 are separated by 162 nucleotides BWI76_RS08550 and BWI76_RS08555 are separated by 26 nucleotides BWI76_RS08555 and BWI76_RS08560 are separated by 3 nucleotides BWI76_RS08560 and BWI76_RS08565 are separated by 144 nucleotides BWI76_RS08565 and BWI76_RS08570 are separated by 35 nucleotides BWI76_RS08570 and BWI76_RS08580 are separated by 456 nucleotides
BWI76_RS08545: BWI76_RS08545 - cell division protein CpoB, at 1,808,345 to 1,809,130
_RS08545
BWI76_RS08550: BWI76_RS08550 - tRNA-Lys, at 1,809,293 to 1,809,368
_RS08550
BWI76_RS08555: BWI76_RS08555 - tRNA-Val, at 1,809,395 to 1,809,470
_RS08555
BWI76_RS08560: BWI76_RS08560 - tRNA-Lys, at 1,809,474 to 1,809,549
_RS08560
BWI76_RS08565: BWI76_RS08565 - tRNA-Lys, at 1,809,694 to 1,809,769
_RS08565
BWI76_RS08570: BWI76_RS08570 - tRNA-Lys, at 1,809,805 to 1,809,880
_RS08570
BWI76_RS08580: BWI76_RS08580 - quinolinate synthetase, at 1,810,337 to 1,811,380
_RS08580
Position (kb)
1809
1810 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1808.797 kb on + strand, within BWI76_RS08545 at 1808.892 kb on - strand, within BWI76_RS08545 at 1809.582 kb on + strand at 1810.013 kb on + strand at 1810.288 kb on - strand at 1810.504 kb on - strand, within BWI76_RS08580
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media; pH 5.5 remove 1,808,797 + BWI76_RS08545 0.58 +0.3 1,808,892 - BWI76_RS08545 0.70 -0.7 1,809,582 + -1.0 1,810,013 + -0.2 1,810,288 - -0.8 1,810,504 - BWI76_RS08580 0.16 -5.0
Or see this region's nucleotide sequence