Experiment: Vogels_fungal_media; pH 5.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06385 and BWI76_RS06390 are separated by 0 nucleotides BWI76_RS06390 and BWI76_RS06395 are separated by 198 nucleotides BWI76_RS06395 and BWI76_RS06400 are separated by 58 nucleotides BWI76_RS06400 and BWI76_RS06405 overlap by 38 nucleotides
BWI76_RS06385: BWI76_RS06385 - (2E,6E)-farnesyl diphosphate synthase, at 1,342,490 to 1,343,389
_RS06385
BWI76_RS06390: BWI76_RS06390 - exodeoxyribonuclease VII small subunit, at 1,343,390 to 1,343,632
_RS06390
BWI76_RS06395: BWI76_RS06395 - tRNA 4-thiouridine(8) synthase ThiI, at 1,343,831 to 1,345,279
_RS06395
BWI76_RS06400: BWI76_RS06400 - oxidative-stress-resistance chaperone, at 1,345,338 to 1,345,928
_RS06400
BWI76_RS06405: BWI76_RS06405 - 2-dehydropantoate 2-reductase, at 1,345,891 to 1,346,802
_RS06405
Position (kb)
1343
1344
1345
1346 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1343.737 kb on + strand at 1343.754 kb on + strand at 1344.077 kb on + strand, within BWI76_RS06395 at 1344.140 kb on + strand, within BWI76_RS06395 at 1344.507 kb on + strand, within BWI76_RS06395 at 1345.113 kb on - strand, within BWI76_RS06395 at 1345.420 kb on - strand, within BWI76_RS06400 at 1345.780 kb on - strand at 1345.835 kb on + strand, within BWI76_RS06400
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media; pH 5.5 remove 1,343,737 + -0.2 1,343,754 + +0.9 1,344,077 + BWI76_RS06395 0.17 -1.8 1,344,140 + BWI76_RS06395 0.21 -1.0 1,344,507 + BWI76_RS06395 0.47 -2.9 1,345,113 - BWI76_RS06395 0.88 -1.2 1,345,420 - BWI76_RS06400 0.14 +0.6 1,345,780 - +1.5 1,345,835 + BWI76_RS06400 0.84 -0.5
Or see this region's nucleotide sequence