Experiment: Vogels_fungal_media; pH 5.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04990 and BWI76_RS04995 are separated by 11 nucleotides BWI76_RS04995 and BWI76_RS05000 are separated by 118 nucleotides BWI76_RS05000 and BWI76_RS05005 overlap by 4 nucleotides
BWI76_RS04990: BWI76_RS04990 - pantoate--beta-alanine ligase, at 1,046,983 to 1,047,837
_RS04990
BWI76_RS04995: BWI76_RS04995 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 1,047,849 to 1,048,640
_RS04995
BWI76_RS05000: BWI76_RS05000 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 1,048,759 to 1,049,238
_RS05000
BWI76_RS05005: BWI76_RS05005 - polynucleotide adenylyltransferase, at 1,049,235 to 1,050,632
_RS05005
Position (kb) 1048
1049
1050 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1048.361 kb on - strand, within BWI76_RS04995 at 1048.377 kb on - strand, within BWI76_RS04995 at 1048.451 kb on + strand, within BWI76_RS04995 at 1048.477 kb on - strand, within BWI76_RS04995 at 1048.482 kb on + strand, within BWI76_RS04995 at 1049.808 kb on - strand, within BWI76_RS05005
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media; pH 5.5 remove 1,048,361 - BWI76_RS04995 0.65 -1.9 1,048,377 - BWI76_RS04995 0.67 -3.1 1,048,451 + BWI76_RS04995 0.76 -2.0 1,048,477 - BWI76_RS04995 0.79 -1.9 1,048,482 + BWI76_RS04995 0.80 -2.6 1,049,808 - BWI76_RS05005 0.41 -0.6
Or see this region's nucleotide sequence