Experiment: Vogels_fungal_media; pH 5.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01545 and BWI76_RS01550 overlap by 8 nucleotides BWI76_RS01550 and BWI76_RS01555 overlap by 1 nucleotides BWI76_RS01555 and BWI76_RS01560 are separated by 253 nucleotides BWI76_RS01560 and BWI76_RS01565 are separated by 95 nucleotides
BWI76_RS01545: BWI76_RS01545 - thiamine biosynthesis protein ThiS, at 314,640 to 315,395
_RS01545
BWI76_RS01550: BWI76_RS01550 - thiamine phosphate synthase, at 315,388 to 316,023
_RS01550
BWI76_RS01555: BWI76_RS01555 - phosphomethylpyrimidine synthase ThiC, at 316,023 to 317,918
_RS01555
BWI76_RS01560: BWI76_RS01560 - Rsd/AlgQ family anti-sigma factor, at 318,172 to 318,669
_RS01560
BWI76_RS01565: BWI76_RS01565 - NADH pyrophosphatase, at 318,765 to 319,538
_RS01565
Position (kb) 316
317
318 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 315.138 kb on - strand, within BWI76_RS01545 at 315.202 kb on - strand, within BWI76_RS01545 at 315.435 kb on + strand at 315.461 kb on + strand, within BWI76_RS01550 at 315.551 kb on - strand, within BWI76_RS01550 at 315.725 kb on - strand, within BWI76_RS01550 at 315.779 kb on + strand, within BWI76_RS01550 at 315.779 kb on + strand, within BWI76_RS01550 at 315.779 kb on + strand, within BWI76_RS01550 at 315.912 kb on + strand, within BWI76_RS01550 at 316.029 kb on - strand at 316.138 kb on - strand at 316.326 kb on + strand, within BWI76_RS01555 at 316.421 kb on + strand, within BWI76_RS01555 at 316.529 kb on + strand, within BWI76_RS01555 at 316.529 kb on + strand, within BWI76_RS01555 at 316.537 kb on - strand, within BWI76_RS01555 at 316.537 kb on - strand, within BWI76_RS01555 at 316.766 kb on - strand, within BWI76_RS01555 at 316.939 kb on + strand, within BWI76_RS01555 at 317.373 kb on + strand, within BWI76_RS01555 at 317.373 kb on + strand, within BWI76_RS01555 at 317.457 kb on - strand, within BWI76_RS01555 at 317.594 kb on - strand, within BWI76_RS01555 at 317.677 kb on + strand, within BWI76_RS01555 at 317.756 kb on + strand at 317.813 kb on + strand at 317.849 kb on + strand at 317.946 kb on - strand at 317.990 kb on - strand at 318.007 kb on + strand at 318.040 kb on - strand at 318.040 kb on - strand at 318.142 kb on + strand at 318.176 kb on - strand at 318.199 kb on + strand at 318.215 kb on - strand at 318.371 kb on + strand, within BWI76_RS01560 at 318.371 kb on + strand, within BWI76_RS01560 at 318.371 kb on + strand, within BWI76_RS01560 at 318.379 kb on - strand, within BWI76_RS01560 at 318.379 kb on - strand, within BWI76_RS01560 at 318.483 kb on - strand, within BWI76_RS01560 at 318.564 kb on + strand, within BWI76_RS01560 at 318.605 kb on - strand, within BWI76_RS01560 at 318.630 kb on + strand at 318.638 kb on - strand at 318.668 kb on - strand at 318.754 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media; pH 5.5 remove 315,138 - BWI76_RS01545 0.66 -0.8 315,202 - BWI76_RS01545 0.74 -1.2 315,435 + -2.8 315,461 + BWI76_RS01550 0.11 -2.9 315,551 - BWI76_RS01550 0.26 -2.8 315,725 - BWI76_RS01550 0.53 -0.9 315,779 + BWI76_RS01550 0.61 -2.3 315,779 + BWI76_RS01550 0.61 -2.4 315,779 + BWI76_RS01550 0.61 -1.5 315,912 + BWI76_RS01550 0.82 -3.4 316,029 - +1.0 316,138 - -1.5 316,326 + BWI76_RS01555 0.16 +0.8 316,421 + BWI76_RS01555 0.21 -1.2 316,529 + BWI76_RS01555 0.27 -1.8 316,529 + BWI76_RS01555 0.27 -1.0 316,537 - BWI76_RS01555 0.27 -1.4 316,537 - BWI76_RS01555 0.27 -0.9 316,766 - BWI76_RS01555 0.39 -2.1 316,939 + BWI76_RS01555 0.48 -3.2 317,373 + BWI76_RS01555 0.71 -1.5 317,373 + BWI76_RS01555 0.71 -2.1 317,457 - BWI76_RS01555 0.76 -1.0 317,594 - BWI76_RS01555 0.83 -1.3 317,677 + BWI76_RS01555 0.87 -3.0 317,756 + -1.8 317,813 + -5.2 317,849 + -3.1 317,946 - -2.6 317,990 - -1.7 318,007 + -1.8 318,040 - -3.5 318,040 - -1.7 318,142 + -1.1 318,176 - +0.0 318,199 + -2.4 318,215 - -1.4 318,371 + BWI76_RS01560 0.40 -1.1 318,371 + BWI76_RS01560 0.40 +0.3 318,371 + BWI76_RS01560 0.40 +0.7 318,379 - BWI76_RS01560 0.42 +0.0 318,379 - BWI76_RS01560 0.42 +1.7 318,483 - BWI76_RS01560 0.62 -0.2 318,564 + BWI76_RS01560 0.79 -0.0 318,605 - BWI76_RS01560 0.87 -1.0 318,630 + +0.1 318,638 - +0.1 318,668 - -1.1 318,754 + +0.8
Or see this region's nucleotide sequence