Experiment: Vogels_fungal_media; pH 5.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01195 and BWI76_RS01200 are separated by 25 nucleotides BWI76_RS01200 and BWI76_RS01205 overlap by 4 nucleotides BWI76_RS01205 and BWI76_RS01210 overlap by 4 nucleotides BWI76_RS01210 and BWI76_RS01215 overlap by 1 nucleotides
BWI76_RS01195: BWI76_RS01195 - membrane protein, at 245,131 to 245,334
_RS01195
BWI76_RS01200: BWI76_RS01200 - diaminopimelate epimerase, at 245,360 to 246,184
_RS01200
BWI76_RS01205: BWI76_RS01205 - DUF484 family protein, at 246,181 to 246,888
_RS01205
BWI76_RS01210: BWI76_RS01210 - tyrosine recombinase XerC, at 246,885 to 247,787
_RS01210
BWI76_RS01215: BWI76_RS01215 - flavin mononucleotide phosphatase, at 247,787 to 248,503
_RS01215
Position (kb) 246
247 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 245.186 kb on + strand, within BWI76_RS01195 at 245.194 kb on - strand, within BWI76_RS01195 at 245.337 kb on + strand at 245.809 kb on - strand, within BWI76_RS01200 at 245.809 kb on - strand, within BWI76_RS01200 at 245.838 kb on + strand, within BWI76_RS01200 at 245.838 kb on + strand, within BWI76_RS01200 at 246.061 kb on - strand, within BWI76_RS01200 at 246.133 kb on + strand at 246.133 kb on + strand at 246.133 kb on + strand at 246.233 kb on + strand at 246.238 kb on + strand at 246.744 kb on + strand, within BWI76_RS01205 at 246.900 kb on - strand at 247.091 kb on + strand, within BWI76_RS01210 at 247.448 kb on + strand, within BWI76_RS01210 at 247.460 kb on + strand, within BWI76_RS01210 at 247.835 kb on - strand at 247.857 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media; pH 5.5 remove 245,186 + BWI76_RS01195 0.27 +0.3 245,194 - BWI76_RS01195 0.31 -2.6 245,337 + +0.5 245,809 - BWI76_RS01200 0.54 -0.8 245,809 - BWI76_RS01200 0.54 -2.2 245,838 + BWI76_RS01200 0.58 -0.5 245,838 + BWI76_RS01200 0.58 +1.8 246,061 - BWI76_RS01200 0.85 -1.0 246,133 + -1.2 246,133 + -0.5 246,133 + -2.2 246,233 + +0.8 246,238 + -0.6 246,744 + BWI76_RS01205 0.80 -1.4 246,900 - -3.1 247,091 + BWI76_RS01210 0.23 -4.1 247,448 + BWI76_RS01210 0.62 -5.1 247,460 + BWI76_RS01210 0.64 -2.9 247,835 - +0.2 247,857 + +0.6
Or see this region's nucleotide sequence