Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08205 and BWI76_RS08210 are separated by 8 nucleotides BWI76_RS08210 and BWI76_RS08215 are separated by 60 nucleotides
BWI76_RS08205: BWI76_RS08205 - DNA-binding transcriptional dual regulator NagC, at 1,735,483 to 1,736,703
_RS08205
BWI76_RS08210: BWI76_RS08210 - N-acetylglucosamine-6-phosphate deacetylase, at 1,736,712 to 1,737,860
_RS08210
BWI76_RS08215: BWI76_RS08215 - glucosamine-6-phosphate deaminase, at 1,737,921 to 1,738,721
_RS08215
Position (kb)
1736
1737
1738 Strain fitness (log2 ratio)
-2
-1
0
1 at 1735.730 kb on + strand, within BWI76_RS08205 at 1735.915 kb on + strand, within BWI76_RS08205 at 1736.060 kb on - strand, within BWI76_RS08205 at 1736.300 kb on - strand, within BWI76_RS08205 at 1736.394 kb on - strand, within BWI76_RS08205 at 1736.430 kb on - strand, within BWI76_RS08205 at 1737.060 kb on - strand, within BWI76_RS08210 at 1737.418 kb on - strand, within BWI76_RS08210 at 1738.242 kb on + strand, within BWI76_RS08215 at 1738.483 kb on + strand, within BWI76_RS08215 at 1738.505 kb on + strand, within BWI76_RS08215 at 1738.534 kb on + strand, within BWI76_RS08215 at 1738.696 kb on + strand at 1738.773 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB remove 1,735,730 + BWI76_RS08205 0.20 +0.3 1,735,915 + BWI76_RS08205 0.35 -0.3 1,736,060 - BWI76_RS08205 0.47 -2.0 1,736,300 - BWI76_RS08205 0.67 -0.1 1,736,394 - BWI76_RS08205 0.75 -0.3 1,736,430 - BWI76_RS08205 0.78 -1.1 1,737,060 - BWI76_RS08210 0.30 -2.5 1,737,418 - BWI76_RS08210 0.61 -1.4 1,738,242 + BWI76_RS08215 0.40 +0.5 1,738,483 + BWI76_RS08215 0.70 +0.4 1,738,505 + BWI76_RS08215 0.73 -0.1 1,738,534 + BWI76_RS08215 0.77 +0.2 1,738,696 + -0.1 1,738,773 + -0.5
Or see this region's nucleotide sequence