Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06165 and BWI76_RS06170 are separated by 46 nucleotides BWI76_RS06170 and BWI76_RS06175 are separated by 116 nucleotides BWI76_RS06175 and BWI76_RS06180 overlap by 4 nucleotides
BWI76_RS06165: BWI76_RS06165 - phosphate starvation-inducible protein PsiF, at 1,295,596 to 1,295,916
_RS06165
BWI76_RS06170: BWI76_RS06170 - pyrroline-5-carboxylate reductase, at 1,295,963 to 1,296,772
_RS06170
BWI76_RS06175: BWI76_RS06175 - DUF188 domain-containing protein, at 1,296,889 to 1,297,347
_RS06175
BWI76_RS06180: BWI76_RS06180 - hypothetical protein, at 1,297,344 to 1,298,042
_RS06180
Position (kb)
1296
1297
1298 Strain fitness (log2 ratio)
-2
-1
0
1 at 1296.958 kb on - strand, within BWI76_RS06175 at 1297.039 kb on + strand, within BWI76_RS06175 at 1297.173 kb on + strand, within BWI76_RS06175 at 1297.405 kb on + strand at 1297.546 kb on - strand, within BWI76_RS06180 at 1297.580 kb on - strand, within BWI76_RS06180 at 1297.853 kb on + strand, within BWI76_RS06180 at 1298.038 kb on - strand at 1298.039 kb on + strand at 1298.052 kb on - strand at 1298.125 kb on - strand at 1298.125 kb on - strand at 1298.125 kb on - strand at 1298.321 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB remove 1,296,958 - BWI76_RS06175 0.15 -0.4 1,297,039 + BWI76_RS06175 0.33 -0.9 1,297,173 + BWI76_RS06175 0.62 -1.8 1,297,405 + +0.3 1,297,546 - BWI76_RS06180 0.29 +0.4 1,297,580 - BWI76_RS06180 0.34 +0.4 1,297,853 + BWI76_RS06180 0.73 -0.5 1,298,038 - +0.1 1,298,039 + -0.6 1,298,052 - -1.0 1,298,125 - -1.3 1,298,125 - -0.2 1,298,125 - -0.7 1,298,321 - -0.5
Or see this region's nucleotide sequence