Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18850 and BWI76_RS18855 are separated by 30 nucleotides BWI76_RS18855 and BWI76_RS18860 are separated by 14 nucleotides
BWI76_RS18850: BWI76_RS18850 - iron-sulfur cluster assembly scaffold protein NifU, at 3,910,031 to 3,910,855
_RS18850
BWI76_RS18855: BWI76_RS18855 - cysteine desulfurase NifS, at 3,910,886 to 3,912,088
_RS18855
BWI76_RS18860: BWI76_RS18860 - homocitrate synthase, at 3,912,103 to 3,913,245
_RS18860
Position (kb)
3910
3911
3912
3913 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3910.023 kb on - strand at 3910.273 kb on + strand, within BWI76_RS18850 at 3910.355 kb on - strand, within BWI76_RS18850 at 3910.481 kb on + strand, within BWI76_RS18850 at 3910.481 kb on + strand, within BWI76_RS18850 at 3910.481 kb on + strand, within BWI76_RS18850 at 3910.489 kb on - strand, within BWI76_RS18850 at 3910.709 kb on + strand, within BWI76_RS18850 at 3910.712 kb on - strand, within BWI76_RS18850 at 3910.782 kb on + strand at 3911.309 kb on + strand, within BWI76_RS18855 at 3911.517 kb on + strand, within BWI76_RS18855 at 3911.567 kb on + strand, within BWI76_RS18855 at 3911.838 kb on + strand, within BWI76_RS18855 at 3911.867 kb on - strand, within BWI76_RS18855 at 3912.030 kb on + strand at 3912.096 kb on - strand at 3912.636 kb on + strand, within BWI76_RS18860 at 3912.750 kb on + strand, within BWI76_RS18860 at 3912.954 kb on - strand, within BWI76_RS18860 at 3913.047 kb on + strand, within BWI76_RS18860
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB remove 3,910,023 - -0.2 3,910,273 + BWI76_RS18850 0.29 -0.3 3,910,355 - BWI76_RS18850 0.39 -0.4 3,910,481 + BWI76_RS18850 0.55 +0.6 3,910,481 + BWI76_RS18850 0.55 +0.1 3,910,481 + BWI76_RS18850 0.55 -3.4 3,910,489 - BWI76_RS18850 0.56 -0.2 3,910,709 + BWI76_RS18850 0.82 +0.2 3,910,712 - BWI76_RS18850 0.83 -0.6 3,910,782 + -1.5 3,911,309 + BWI76_RS18855 0.35 +0.5 3,911,517 + BWI76_RS18855 0.52 -0.8 3,911,567 + BWI76_RS18855 0.57 -1.0 3,911,838 + BWI76_RS18855 0.79 +0.1 3,911,867 - BWI76_RS18855 0.82 -1.0 3,912,030 + +1.4 3,912,096 - +0.4 3,912,636 + BWI76_RS18860 0.47 +0.8 3,912,750 + BWI76_RS18860 0.57 -0.5 3,912,954 - BWI76_RS18860 0.74 +0.3 3,913,047 + BWI76_RS18860 0.83 -0.7
Or see this region's nucleotide sequence