Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12545 and BWI76_RS12550 are separated by 14 nucleotides BWI76_RS12550 and BWI76_RS12555 overlap by 4 nucleotides BWI76_RS12555 and BWI76_RS12560 overlap by 4 nucleotides
BWI76_RS12545: BWI76_RS12545 - hypothetical protein, at 2,621,007 to 2,621,966
_RS12545
BWI76_RS12550: BWI76_RS12550 - hypothetical protein, at 2,621,981 to 2,622,514
_RS12550
BWI76_RS12555: BWI76_RS12555 - hypothetical protein, at 2,622,511 to 2,623,035
_RS12555
BWI76_RS12560: BWI76_RS12560 - hypothetical protein, at 2,623,032 to 2,623,517
_RS12560
BWI76_RS12565: BWI76_RS12565 - SAM-dependent methyltransferase, at 2,623,514 to 2,624,734
_RS12565
Position (kb)
2621
2622
2623 Strain fitness (log2 ratio)
-2
-1
0
1 at 2621.035 kb on - strand at 2621.048 kb on + strand at 2621.120 kb on + strand, within BWI76_RS12545 at 2621.475 kb on - strand, within BWI76_RS12545 at 2621.475 kb on - strand, within BWI76_RS12545 at 2622.111 kb on + strand, within BWI76_RS12550 at 2622.264 kb on - strand, within BWI76_RS12550 at 2622.699 kb on - strand, within BWI76_RS12555 at 2622.699 kb on - strand, within BWI76_RS12555 at 2622.806 kb on + strand, within BWI76_RS12555 at 2622.814 kb on - strand, within BWI76_RS12555 at 2622.838 kb on - strand, within BWI76_RS12555 at 2623.224 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB remove 2,621,035 - -0.3 2,621,048 + -0.8 2,621,120 + BWI76_RS12545 0.12 -1.8 2,621,475 - BWI76_RS12545 0.49 -1.0 2,621,475 - BWI76_RS12545 0.49 -0.2 2,622,111 + BWI76_RS12550 0.24 +0.3 2,622,264 - BWI76_RS12550 0.53 +1.0 2,622,699 - BWI76_RS12555 0.36 +0.8 2,622,699 - BWI76_RS12555 0.36 +0.5 2,622,806 + BWI76_RS12555 0.56 +0.2 2,622,814 - BWI76_RS12555 0.58 -0.1 2,622,838 - BWI76_RS12555 0.62 +0.3 2,623,224 + +0.1
Or see this region's nucleotide sequence