Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11570 and BWI76_RS11575 are separated by 130 nucleotides BWI76_RS11575 and BWI76_RS11580 are separated by 10 nucleotides BWI76_RS11580 and BWI76_RS11585 are separated by 94 nucleotides
BWI76_RS11570: BWI76_RS11570 - MFS transporter, at 2,434,019 to 2,435,374
_RS11570
BWI76_RS11575: BWI76_RS11575 - nicotinamidase/pyrazinamidase, at 2,435,505 to 2,436,146
_RS11575
BWI76_RS11580: BWI76_RS11580 - L-asparaginase 1, at 2,436,157 to 2,437,173
_RS11580
BWI76_RS11585: BWI76_RS11585 - signal peptide peptidase A, at 2,437,268 to 2,439,121
_RS11585
Position (kb)
2436
2437
2438 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2435.714 kb on + strand, within BWI76_RS11575 at 2435.877 kb on + strand, within BWI76_RS11575 at 2435.917 kb on - strand at 2435.947 kb on + strand, within BWI76_RS11575 at 2435.955 kb on - strand, within BWI76_RS11575 at 2436.064 kb on + strand, within BWI76_RS11575 at 2436.071 kb on - strand, within BWI76_RS11575 at 2436.375 kb on + strand, within BWI76_RS11580 at 2436.931 kb on - strand, within BWI76_RS11580 at 2437.185 kb on + strand at 2437.206 kb on + strand at 2437.206 kb on + strand at 2437.206 kb on + strand at 2437.209 kb on - strand at 2437.214 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB remove 2,435,714 + BWI76_RS11575 0.33 +0.5 2,435,877 + BWI76_RS11575 0.58 -0.0 2,435,917 - +2.2 2,435,947 + BWI76_RS11575 0.69 -0.3 2,435,955 - BWI76_RS11575 0.70 +0.5 2,436,064 + BWI76_RS11575 0.87 +0.5 2,436,071 - BWI76_RS11575 0.88 +0.8 2,436,375 + BWI76_RS11580 0.21 -3.6 2,436,931 - BWI76_RS11580 0.76 -1.3 2,437,185 + +0.6 2,437,206 + -0.3 2,437,206 + +3.4 2,437,206 + +0.5 2,437,209 - -0.4 2,437,214 - -0.1
Or see this region's nucleotide sequence