Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04890

Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04885 and BWI76_RS04890 are separated by 207 nucleotidesBWI76_RS04890 and BWI76_RS04895 are separated by 162 nucleotidesBWI76_RS04895 and BWI76_RS04900 are separated by 50 nucleotides BWI76_RS04885: BWI76_RS04885 - pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase, at 1,022,684 to 1,024,573 _RS04885 BWI76_RS04890: BWI76_RS04890 - dihydrolipoyl dehydrogenase, at 1,024,781 to 1,026,205 _RS04890 BWI76_RS04895: BWI76_RS04895 - hypothetical protein, at 1,026,368 to 1,026,652 _RS04895 BWI76_RS04900: BWI76_RS04900 - DUF2950 domain-containing protein, at 1,026,703 to 1,027,497 _RS04900 Position (kb) 1024 1025 1026 1027Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1026.265 kb on + strandat 1026.273 kb on - strandat 1026.273 kb on - strandat 1026.384 kb on - strandat 1026.400 kb on - strand, within BWI76_RS04895at 1026.416 kb on + strand, within BWI76_RS04895at 1026.424 kb on - strand, within BWI76_RS04895at 1026.944 kb on - strand, within BWI76_RS04900

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Per-strain Table

Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB
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1,026,265 + +0.3
1,026,273 - +2.3
1,026,273 - -1.0
1,026,384 - +1.8
1,026,400 - BWI76_RS04895 0.11 -3.6
1,026,416 + BWI76_RS04895 0.17 +2.0
1,026,424 - BWI76_RS04895 0.20 +0.6
1,026,944 - BWI76_RS04900 0.30 -0.2

Or see this region's nucleotide sequence