Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15210 and BWI76_RS15215 overlap by 47 nucleotides BWI76_RS15215 and BWI76_RS15220 are separated by 145 nucleotides BWI76_RS15220 and BWI76_RS15225 are separated by 12 nucleotides BWI76_RS15225 and BWI76_RS15230 are separated by 1 nucleotides
BWI76_RS15210: BWI76_RS15210 - LysR family transcriptional regulator, at 3,188,634 to 3,189,512
_RS15210
BWI76_RS15215: BWI76_RS15215 - N-acetyltransferase, at 3,189,466 to 3,189,948
_RS15215
BWI76_RS15220: BWI76_RS15220 - L-lactate dehydrogenase, at 3,190,094 to 3,191,038
_RS15220
BWI76_RS15225: BWI76_RS15225 - DeoR family transcriptional regulator, at 3,191,051 to 3,191,812
_RS15225
BWI76_RS15230: BWI76_RS15230 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 3,191,814 to 3,192,800
_RS15230
Position (kb)
3190
3191
3192 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3189.178 kb on + strand, within BWI76_RS15210 at 3189.322 kb on + strand, within BWI76_RS15210 at 3189.351 kb on - strand, within BWI76_RS15210 at 3189.595 kb on + strand, within BWI76_RS15215 at 3190.233 kb on + strand, within BWI76_RS15220 at 3190.611 kb on + strand, within BWI76_RS15220 at 3190.619 kb on - strand, within BWI76_RS15220 at 3191.144 kb on + strand, within BWI76_RS15225 at 3191.152 kb on - strand, within BWI76_RS15225 at 3191.276 kb on - strand, within BWI76_RS15225 at 3191.685 kb on + strand, within BWI76_RS15225 at 3191.693 kb on - strand, within BWI76_RS15225 at 3191.848 kb on - strand at 3191.851 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X remove 3,189,178 + BWI76_RS15210 0.62 +0.0 3,189,322 + BWI76_RS15210 0.78 +0.7 3,189,351 - BWI76_RS15210 0.82 +1.0 3,189,595 + BWI76_RS15215 0.27 +1.2 3,190,233 + BWI76_RS15220 0.15 -0.2 3,190,611 + BWI76_RS15220 0.55 -1.5 3,190,619 - BWI76_RS15220 0.56 -0.2 3,191,144 + BWI76_RS15225 0.12 -0.4 3,191,152 - BWI76_RS15225 0.13 -5.2 3,191,276 - BWI76_RS15225 0.30 -1.1 3,191,685 + BWI76_RS15225 0.83 -2.6 3,191,693 - BWI76_RS15225 0.84 -5.3 3,191,848 - -0.2 3,191,851 - +0.3
Or see this region's nucleotide sequence