Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01170

Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01165 and BWI76_RS01170 are separated by 3 nucleotidesBWI76_RS01170 and BWI76_RS01175 are separated by 21 nucleotidesBWI76_RS01175 and BWI76_RS01180 overlap by 4 nucleotides BWI76_RS01165: BWI76_RS01165 - protoheme IX biogenesis protein HemY, at 237,626 to 238,822 _RS01165 BWI76_RS01170: BWI76_RS01170 - uroporphyrinogen-III C-methyltransferase, at 238,826 to 239,995 _RS01170 BWI76_RS01175: BWI76_RS01175 - uroporphyrinogen-III synthase, at 240,017 to 240,757 _RS01175 BWI76_RS01180: BWI76_RS01180 - porphobilinogen deaminase, at 240,754 to 241,695 _RS01180 Position (kb) 238 239 240Strain fitness (log2 ratio) -3 -2 -1 0 1at 237.830 kb on + strand, within BWI76_RS01165at 237.855 kb on + strand, within BWI76_RS01165at 237.889 kb on - strand, within BWI76_RS01165at 238.154 kb on + strand, within BWI76_RS01165at 238.162 kb on - strand, within BWI76_RS01165at 238.230 kb on + strand, within BWI76_RS01165at 238.564 kb on + strand, within BWI76_RS01165at 238.944 kb on - strand, within BWI76_RS01170at 239.119 kb on - strand, within BWI76_RS01170at 239.155 kb on + strand, within BWI76_RS01170at 239.528 kb on + strand, within BWI76_RS01170at 239.545 kb on + strand, within BWI76_RS01170at 239.939 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X
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237,830 + BWI76_RS01165 0.17 -1.3
237,855 + BWI76_RS01165 0.19 +0.5
237,889 - BWI76_RS01165 0.22 +0.2
238,154 + BWI76_RS01165 0.44 +0.0
238,162 - BWI76_RS01165 0.45 +0.5
238,230 + BWI76_RS01165 0.50 -0.6
238,564 + BWI76_RS01165 0.78 -0.2
238,944 - BWI76_RS01170 0.10 -2.3
239,119 - BWI76_RS01170 0.25 -2.9
239,155 + BWI76_RS01170 0.28 -1.3
239,528 + BWI76_RS01170 0.60 -3.6
239,545 + BWI76_RS01170 0.61 -0.9
239,939 + -0.0

Or see this region's nucleotide sequence