Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS11880

Experiment: Ferulic Acid 2 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS11870 and PFLU_RS11875 are separated by 47 nucleotidesPFLU_RS11875 and PFLU_RS11880 overlap by 4 nucleotidesPFLU_RS11880 and PFLU_RS11885 overlap by 8 nucleotidesPFLU_RS11885 and PFLU_RS11890 overlap by 4 nucleotides PFLU_RS11870: PFLU_RS11870 - type II secretion system protein GspG, at 2,627,878 to 2,628,327 _RS11870 PFLU_RS11875: PFLU_RS11875 - general secretion pathway protein GspK, at 2,628,375 to 2,629,205 _RS11875 PFLU_RS11880: PFLU_RS11880 - general secretion pathway protein GspL, at 2,629,202 to 2,630,263 _RS11880 PFLU_RS11885: PFLU_RS11885 - type II secretion system protein M, at 2,630,256 to 2,630,768 _RS11885 PFLU_RS11890: PFLU_RS11890 - type II secretion system secretin GspD, at 2,630,765 to 2,633,077 _RS11890 Position (kb) 2629 2630 2631Strain fitness (log2 ratio) -2 -1 0 1 2at 2628.214 kb on + strand, within PFLU_RS11870at 2628.214 kb on + strand, within PFLU_RS11870at 2628.215 kb on - strand, within PFLU_RS11870at 2628.220 kb on + strand, within PFLU_RS11870at 2628.325 kb on + strandat 2628.325 kb on + strandat 2628.325 kb on + strandat 2628.325 kb on + strandat 2628.325 kb on + strandat 2628.325 kb on + strandat 2628.326 kb on - strandat 2628.326 kb on - strandat 2628.326 kb on - strandat 2628.326 kb on - strandat 2628.775 kb on - strand, within PFLU_RS11875at 2628.839 kb on - strand, within PFLU_RS11875at 2628.839 kb on - strand, within PFLU_RS11875at 2628.870 kb on + strand, within PFLU_RS11875at 2628.870 kb on + strand, within PFLU_RS11875at 2628.870 kb on + strand, within PFLU_RS11875at 2628.870 kb on + strand, within PFLU_RS11875at 2628.870 kb on + strand, within PFLU_RS11875at 2628.871 kb on - strand, within PFLU_RS11875at 2628.871 kb on - strand, within PFLU_RS11875at 2628.871 kb on - strand, within PFLU_RS11875at 2628.871 kb on - strand, within PFLU_RS11875at 2628.893 kb on + strand, within PFLU_RS11875at 2628.894 kb on - strand, within PFLU_RS11875at 2628.894 kb on - strand, within PFLU_RS11875at 2629.080 kb on - strand, within PFLU_RS11875at 2629.080 kb on - strand, within PFLU_RS11875at 2629.305 kb on - strandat 2629.305 kb on - strandat 2629.327 kb on + strand, within PFLU_RS11880at 2629.327 kb on + strand, within PFLU_RS11880at 2629.328 kb on - strand, within PFLU_RS11880at 2629.452 kb on + strand, within PFLU_RS11880at 2629.596 kb on - strand, within PFLU_RS11880at 2629.596 kb on - strand, within PFLU_RS11880at 2629.635 kb on - strand, within PFLU_RS11880at 2629.853 kb on + strand, within PFLU_RS11880at 2629.853 kb on + strand, within PFLU_RS11880at 2630.048 kb on - strand, within PFLU_RS11880at 2630.261 kb on + strandat 2630.377 kb on + strand, within PFLU_RS11885at 2630.548 kb on - strand, within PFLU_RS11885at 2630.952 kb on + strandat 2631.123 kb on + strand, within PFLU_RS11890at 2631.176 kb on + strand, within PFLU_RS11890

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Per-strain Table

Position Strand Gene LocusTag Fraction Ferulic Acid 2 mM (C)
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2,628,214 + PFLU_RS11870 0.75 -0.8
2,628,214 + PFLU_RS11870 0.75 +0.5
2,628,215 - PFLU_RS11870 0.75 +1.6
2,628,220 + PFLU_RS11870 0.76 +0.4
2,628,325 + -1.5
2,628,325 + +1.2
2,628,325 + +0.6
2,628,325 + -1.4
2,628,325 + -1.7
2,628,325 + -1.6
2,628,326 - +0.9
2,628,326 - -1.1
2,628,326 - -0.2
2,628,326 - +0.6
2,628,775 - PFLU_RS11875 0.48 +0.6
2,628,839 - PFLU_RS11875 0.56 -1.1
2,628,839 - PFLU_RS11875 0.56 +1.7
2,628,870 + PFLU_RS11875 0.60 -2.5
2,628,870 + PFLU_RS11875 0.60 +0.5
2,628,870 + PFLU_RS11875 0.60 +1.5
2,628,870 + PFLU_RS11875 0.60 -2.7
2,628,870 + PFLU_RS11875 0.60 +1.5
2,628,871 - PFLU_RS11875 0.60 -1.6
2,628,871 - PFLU_RS11875 0.60 +0.5
2,628,871 - PFLU_RS11875 0.60 +0.5
2,628,871 - PFLU_RS11875 0.60 +1.2
2,628,893 + PFLU_RS11875 0.62 -0.1
2,628,894 - PFLU_RS11875 0.62 +0.2
2,628,894 - PFLU_RS11875 0.62 -0.4
2,629,080 - PFLU_RS11875 0.85 -0.0
2,629,080 - PFLU_RS11875 0.85 -0.2
2,629,305 - +0.4
2,629,305 - +0.9
2,629,327 + PFLU_RS11880 0.12 +2.7
2,629,327 + PFLU_RS11880 0.12 +0.1
2,629,328 - PFLU_RS11880 0.12 +0.9
2,629,452 + PFLU_RS11880 0.24 -0.3
2,629,596 - PFLU_RS11880 0.37 +0.9
2,629,596 - PFLU_RS11880 0.37 -0.1
2,629,635 - PFLU_RS11880 0.41 -0.4
2,629,853 + PFLU_RS11880 0.61 +1.4
2,629,853 + PFLU_RS11880 0.61 +0.7
2,630,048 - PFLU_RS11880 0.80 -1.0
2,630,261 + -1.0
2,630,377 + PFLU_RS11885 0.24 -2.0
2,630,548 - PFLU_RS11885 0.57 -0.4
2,630,952 + +1.8
2,631,123 + PFLU_RS11890 0.15 -2.2
2,631,176 + PFLU_RS11890 0.18 -2.3

Or see this region's nucleotide sequence