Experiment: SJC25_Acapsular_0.1MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3586 and BT3587 are separated by 6 nucleotides BT3587 and BT3588 overlap by 20 nucleotides
BT3586: BT3586 - putative dehydrogenase (NCBI ptt file), at 4,640,662 to 4,641,666
BT3586
BT3587: BT3587 - glucosamine-6-phosphate isomerase (NCBI ptt file), at 4,641,673 to 4,642,458
BT3587
BT3588: BT3588 - N-acetylglucosamine-6-phosphate deacetylase (NCBI ptt file), at 4,642,439 to 4,643,626
BT3588
Position (kb)
4641
4642
4643 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4640.862 kb on - strand, within BT3586 at 4640.870 kb on + strand, within BT3586 at 4640.871 kb on - strand, within BT3586 at 4640.884 kb on + strand, within BT3586 at 4640.902 kb on - strand, within BT3586 at 4640.903 kb on + strand, within BT3586 at 4640.903 kb on + strand, within BT3586 at 4640.903 kb on + strand, within BT3586 at 4640.952 kb on + strand, within BT3586 at 4640.952 kb on + strand, within BT3586 at 4640.980 kb on + strand, within BT3586 at 4641.009 kb on + strand, within BT3586 at 4641.009 kb on + strand, within BT3586 at 4641.045 kb on + strand, within BT3586 at 4641.045 kb on + strand, within BT3586 at 4641.074 kb on + strand, within BT3586 at 4641.104 kb on - strand, within BT3586 at 4641.119 kb on + strand, within BT3586 at 4641.208 kb on - strand, within BT3586 at 4641.271 kb on - strand, within BT3586 at 4641.284 kb on + strand, within BT3586 at 4641.285 kb on - strand, within BT3586 at 4641.308 kb on - strand, within BT3586 at 4641.335 kb on - strand, within BT3586 at 4641.337 kb on - strand, within BT3586 at 4641.367 kb on + strand, within BT3586 at 4641.368 kb on - strand, within BT3586 at 4641.399 kb on + strand, within BT3586 at 4641.430 kb on + strand, within BT3586 at 4641.431 kb on - strand, within BT3586 at 4641.433 kb on - strand, within BT3586 at 4641.435 kb on - strand, within BT3586 at 4641.435 kb on - strand, within BT3586 at 4641.480 kb on - strand, within BT3586 at 4641.493 kb on + strand, within BT3586 at 4641.590 kb on - strand at 4641.595 kb on - strand at 4641.652 kb on + strand at 4641.670 kb on + strand at 4641.671 kb on - strand at 4641.671 kb on - strand at 4641.674 kb on + strand at 4641.675 kb on - strand at 4641.710 kb on + strand at 4641.710 kb on + strand at 4641.711 kb on - strand at 4641.785 kb on + strand, within BT3587 at 4641.788 kb on - strand, within BT3587 at 4641.842 kb on - strand, within BT3587 at 4641.859 kb on - strand, within BT3587 at 4641.954 kb on + strand, within BT3587 at 4641.955 kb on - strand, within BT3587 at 4641.982 kb on + strand, within BT3587 at 4641.983 kb on - strand, within BT3587 at 4642.005 kb on + strand, within BT3587 at 4642.120 kb on + strand, within BT3587 at 4642.206 kb on - strand, within BT3587 at 4642.260 kb on + strand, within BT3587 at 4642.271 kb on - strand, within BT3587 at 4642.271 kb on - strand, within BT3587 at 4642.390 kb on - strand at 4642.414 kb on + strand at 4642.449 kb on + strand at 4642.482 kb on - strand at 4642.522 kb on + strand at 4642.605 kb on - strand, within BT3588 at 4642.622 kb on - strand, within BT3588 at 4642.637 kb on + strand, within BT3588 at 4642.697 kb on + strand, within BT3588 at 4642.711 kb on + strand, within BT3588 at 4642.715 kb on - strand, within BT3588 at 4642.822 kb on + strand, within BT3588 at 4642.827 kb on - strand, within BT3588 at 4642.877 kb on + strand, within BT3588 at 4642.978 kb on + strand, within BT3588 at 4642.978 kb on + strand, within BT3588 at 4642.979 kb on - strand, within BT3588 at 4642.984 kb on + strand, within BT3588 at 4642.985 kb on - strand, within BT3588 at 4642.986 kb on + strand, within BT3588 at 4643.062 kb on + strand, within BT3588 at 4643.110 kb on + strand, within BT3588 at 4643.111 kb on - strand, within BT3588 at 4643.120 kb on + strand, within BT3588 at 4643.120 kb on + strand, within BT3588 at 4643.144 kb on + strand, within BT3588 at 4643.144 kb on + strand, within BT3588 at 4643.152 kb on + strand, within BT3588 at 4643.152 kb on + strand, within BT3588 at 4643.155 kb on - strand, within BT3588 at 4643.221 kb on - strand, within BT3588 at 4643.245 kb on + strand, within BT3588 at 4643.267 kb on - strand, within BT3588 at 4643.307 kb on - strand, within BT3588 at 4643.339 kb on + strand, within BT3588 at 4643.340 kb on - strand, within BT3588 at 4643.340 kb on - strand, within BT3588 at 4643.411 kb on - strand, within BT3588 at 4643.441 kb on + strand, within BT3588 at 4643.441 kb on + strand, within BT3588 at 4643.441 kb on + strand, within BT3588 at 4643.441 kb on + strand, within BT3588 at 4643.442 kb on - strand, within BT3588 at 4643.442 kb on - strand, within BT3588 at 4643.442 kb on - strand, within BT3588 at 4643.442 kb on - strand, within BT3588
Per-strain Table
Position Strand Gene LocusTag Fraction SJC25_Acapsular_0.1MOI remove 4,640,862 - BT3586 0.20 -1.6 4,640,870 + BT3586 0.21 +1.2 4,640,871 - BT3586 0.21 +0.1 4,640,884 + BT3586 0.22 +1.0 4,640,902 - BT3586 0.24 -1.0 4,640,903 + BT3586 0.24 +0.3 4,640,903 + BT3586 0.24 -2.2 4,640,903 + BT3586 0.24 +0.7 4,640,952 + BT3586 0.29 -1.6 4,640,952 + BT3586 0.29 -0.9 4,640,980 + BT3586 0.32 -0.4 4,641,009 + BT3586 0.35 +1.4 4,641,009 + BT3586 0.35 +0.9 4,641,045 + BT3586 0.38 +0.7 4,641,045 + BT3586 0.38 -0.1 4,641,074 + BT3586 0.41 +0.9 4,641,104 - BT3586 0.44 -0.1 4,641,119 + BT3586 0.45 -2.1 4,641,208 - BT3586 0.54 -0.3 4,641,271 - BT3586 0.61 -1.1 4,641,284 + BT3586 0.62 -0.9 4,641,285 - BT3586 0.62 -1.4 4,641,308 - BT3586 0.64 -0.1 4,641,335 - BT3586 0.67 -0.2 4,641,337 - BT3586 0.67 -0.3 4,641,367 + BT3586 0.70 -2.1 4,641,368 - BT3586 0.70 -2.1 4,641,399 + BT3586 0.73 -0.3 4,641,430 + BT3586 0.76 -1.1 4,641,431 - BT3586 0.77 -0.4 4,641,433 - BT3586 0.77 -2.4 4,641,435 - BT3586 0.77 -1.2 4,641,435 - BT3586 0.77 -0.1 4,641,480 - BT3586 0.81 -0.9 4,641,493 + BT3586 0.83 +1.3 4,641,590 - +0.6 4,641,595 - -0.3 4,641,652 + -0.8 4,641,670 + -1.9 4,641,671 - -0.6 4,641,671 - -1.2 4,641,674 + +0.9 4,641,675 - -1.0 4,641,710 + -0.7 4,641,710 + -2.4 4,641,711 - +0.7 4,641,785 + BT3587 0.14 +1.2 4,641,788 - BT3587 0.15 +1.4 4,641,842 - BT3587 0.22 -0.3 4,641,859 - BT3587 0.24 +0.2 4,641,954 + BT3587 0.36 -0.6 4,641,955 - BT3587 0.36 +1.4 4,641,982 + BT3587 0.39 +1.9 4,641,983 - BT3587 0.39 +1.6 4,642,005 + BT3587 0.42 -1.1 4,642,120 + BT3587 0.57 -1.2 4,642,206 - BT3587 0.68 +1.2 4,642,260 + BT3587 0.75 -2.8 4,642,271 - BT3587 0.76 -0.4 4,642,271 - BT3587 0.76 +0.5 4,642,390 - -2.8 4,642,414 + +0.2 4,642,449 + -0.7 4,642,482 - -1.4 4,642,522 + -2.1 4,642,605 - BT3588 0.14 -1.9 4,642,622 - BT3588 0.15 -1.0 4,642,637 + BT3588 0.17 +0.5 4,642,697 + BT3588 0.22 -0.7 4,642,711 + BT3588 0.23 -1.2 4,642,715 - BT3588 0.23 -2.2 4,642,822 + BT3588 0.32 -1.4 4,642,827 - BT3588 0.33 +0.6 4,642,877 + BT3588 0.37 -1.8 4,642,978 + BT3588 0.45 -0.4 4,642,978 + BT3588 0.45 +0.4 4,642,979 - BT3588 0.45 -0.5 4,642,984 + BT3588 0.46 -0.2 4,642,985 - BT3588 0.46 -0.2 4,642,986 + BT3588 0.46 +0.7 4,643,062 + BT3588 0.52 -2.5 4,643,110 + BT3588 0.56 -1.1 4,643,111 - BT3588 0.57 -0.4 4,643,120 + BT3588 0.57 -2.9 4,643,120 + BT3588 0.57 +0.1 4,643,144 + BT3588 0.59 +0.2 4,643,144 + BT3588 0.59 +0.9 4,643,152 + BT3588 0.60 -1.3 4,643,152 + BT3588 0.60 -2.3 4,643,155 - BT3588 0.60 -1.1 4,643,221 - BT3588 0.66 -0.7 4,643,245 + BT3588 0.68 +0.8 4,643,267 - BT3588 0.70 -0.8 4,643,307 - BT3588 0.73 -0.6 4,643,339 + BT3588 0.76 +0.9 4,643,340 - BT3588 0.76 +0.4 4,643,340 - BT3588 0.76 -0.5 4,643,411 - BT3588 0.82 -0.6 4,643,441 + BT3588 0.84 +1.2 4,643,441 + BT3588 0.84 +1.3 4,643,441 + BT3588 0.84 -0.6 4,643,441 + BT3588 0.84 -2.1 4,643,442 - BT3588 0.84 +1.5 4,643,442 - BT3588 0.84 -1.9 4,643,442 - BT3588 0.84 +1.6 4,643,442 - BT3588 0.84 +1.9
Or see this region's nucleotide sequence